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What are the components of Antibiotic-Antimycotic (100X)? Product FAQ

Answer

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Answer Id:: E17465

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What is the solvent used in Antibiotic-Antimycotic (100X)? Product FAQ

Answer

The components are in a 0.85% saline solution.

Answer Id:: E17466

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Our lab has purchased Antibiotic-Antimycotic (100X). How should I use it? Should I directly add 5 mL of Antibiotic-Antimycotic (100X) to 500 mL cell culture medium or should I dilute the Antibiotic-Antimycotic (100X) 100 times and then add 5 ml of the diluted Antibiotic-Antimycotic to 500 ml of cell culture medium? Product FAQ

Answer

The accurate way would be to dilute 5 mL of Antibiotic-Antimycotic (100X) into 495 mL of medium. Please note that the volume of medium in a 500 mL medium bottle is not exactly 500 mL. It could range from 500.5 - 510.5 mL.

Answer Id:: E17386

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ANTIBIOTIC ANTIMYCOTIC SDS

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Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells? Product FAQ

Answer

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Answer Id:: E17475

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What is the recommended storage condition for Antibiotic-Antimycotic (100X)? Product FAQ

Answer

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of manufacture.

Answer Id:: E17420

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ANTIBIOTIC ANTIMYCOTIC SDS

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My cultures are contaminated, but they are very important and irreplaceable, what is your recommendation? Product FAQ

Answer

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. There is protocol on our web site, which you can find by searching "Use of Antibiotics and Antimycotics" using our website search engine. Click on the resulting links to find the protocol.

Answer Id:: E5548

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My initial primary culture is contaminated, what can I do? Product FAQ

Answer

Contamination of primary tissue could possibly be carried over to culture preparation. Change the media and treat with antibiotics to eliminate the contaminants. To prevent this in the future, wash tissue pieces several times in a balanced salt solution containing a higher concentration of antibiotics and antimycotics before starting culture.

Answer Id:: E3986

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Will bacterial, yeast or fungal contaminants cause pH shifts in my media? Product FAQ

Answer

Absolutely. Discard culture and medium. Recovery is an option, however this is a timely and costly approach using antibiotics and antimycotics.

Answer Id:: E3962

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Can antibiotics be used with StemFlex Medium to prevent contamination during gene editing/flow sorting experiments? Product FAQ

Answer

Yes. We use Antibiotic-Antimycotic (Cat. No. 15240062) for additional protection from contamination.

Answer Id:: E14763

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Can I use pen/strep or other antibiotics for episomal reprogramming of cells? Product FAQ

Answer

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Answer Id:: E6417

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What factors can contribute to rapid cell death/culture failure? Product FAQ

Answer

There are a number of events that can contribute to this:

1. Incorrect CO2 levels-monitor the level of CO2 manually with a Fyrite kit, available from Bacharach (http://www.bacharach-inc.com/fyrite_analyzers.htm). Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to insure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors.
2. Temperature fluctuations in the incubator-Monitor the temperature of incubator with a good thermometer inside the incubator.
3. Fungizone or other preventive antibiotics/antimycotics are present at toxic concentrations-use at recommended levels.
4. Humidity is incorrect-check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media (i.e., appropriate CO2 levels are largely irrelevant for most cultures if the humidity level is not high enough).
5. Incorrect osmotic pressure in medium-check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality.
6. Contamination by microorganisms-bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination.
7. Inappropriate medium is being used-double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.

Answer Id:: E11905

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Bombyxin is a growth factor for wing imaginal disks in Lepidoptera. Citations & References

  • Authors: Nijhout H Frederik; Grunert Laura W;
  • Journal: Proc Natl Acad Sci U S A (2002) 99:15446-15496
  • PubMed ID: 12429853
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Apoptosis induces efflux of the mitochondrial matrix enzyme deoxyguanosine kinase. Citations & References

  • Authors: Jüllig M, Eriksson S,
  • Journal: J Biol Chem (2001) 276:24000-24004
  • PubMed ID: 11294860
Catalog #
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