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Streptavidin-based amplification techniques are widely used in flow cytometry, fluorescent imaging, western blotting, and microplate-based detection for increased signal output and greater sensitivity.

Brian D. Wolf, Ph.D.; Douglas A. Hayworth, Ph.D., In 2009, we updated and streamlined the structure of our immunogenic carrier protein products and conjugation kits. In doing so, we performed a number of experiments designed to validate and demonstrate the quality, properties, differences and uses...

We offer keyhole limpet hemocyanin (KLH) and other carrier proteins to conjugate peptides and haptens through sulfhydryl, amine or carboxyl groups to elicit the production of specific antibodies against almost any molecule. We also offer adjuvants for effective immunization.

Take Advantage of Our Protein Conjugation Experience Table 1 shows a summary of Molecular Probes® protein labeling kits—including the APEX® and Zenon® antibody labeling kits and our range of Alexa Fluor® protein labeling kits—with emphasis on the different starting material requirements and...

Protein A, Protein G, Protein A/G and Protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulin molecules. These proteins are available in purified, salt-free, lyophilized form, as well as, coated in microplates and covalently immobilized to various...

(TOP), Heterobifunctional crosslinkers, Explore protein crosslinking products Crosslinking Technical Handbook, Crosslinkers can be used to study the structure and composition of proteins in purified or complex samples.

Explore streptavidin/biotin binding conjugates Avidin-Biotin Technical Handbook, Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes.

Label transfer methodology Traditional label transfer reagents Nonradioactive label transfer reagents Recommended reading, Sulfo-SBED Biotin Label Transfer Reagent Iodination Reagents for Proteins Biotin Binding Protein Conjugates, In a typical label transfer reaction, a bait protein reacts with a...

Targeting immune checkpoint protein interactions is one form of cancer immunotherapy that offers a novel way to attack tumor cells. The discovery of those druggable protein targets relies on an in-depth understanding of cell signaling pathways.

As compared with the aminocoumarin derivatives, the Cascade Blue® fluorophore shows less spectral overlap with fluorescein, an important advantage for multicolor applications. In addition, this reactive Cascade Blue® derivative has high absorptivity, is highly fluorescent and, unlike most dyes,...

We offer options for producing phosphospecific antibodies and antibodies specific to other post-translational modifications or activation states of particular proteins as part of our custom antibody production service.

Invitrogen PrimeFlow RNA Assay expands the capability of flow cytometry to measure RNA. Combine PrimeFlow with immunolabeling of both cell-surface and intracellular proteins using fluorophore-conjugated antibodies.

Biotin-labeled secondary antibodies are commonly used in various applications such as western blotting, ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and flow cytometry.

In addition to acting as a cellular scaffold, the cytoskeleton has a role in organelle transport, cell division, motility, and signaling, making it central in both normal and disease processes. To facilitate research on the cytoskeleton, we offer phalloidin conjugates, CellLight and TubulinTracker...

Protein conjugates made with Alexa Fluor dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes.