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Applicationa Note: Determination of Sodium, Potassium, and Calcium in Rice and Wheat Flours using Ion Chromatography Product Literature

Fully-traceable GC injections Product Literature

Application Note: Determination of Four Polar Pesticides in Drinking Water by IC-MS Product Literature

Application Note: Determination of Methanesulfonic Acid in Busulfan by Ion Chromatography Product Literature

When I started my run on the Applied Biosystems 310 Genetic Analyzer, an error occurred: "Alert from Instrument: Electrophoresis current is fluctuating - possible bubbles in capillary" but I don't see any bubbles. What else can cause this? Product FAQ

Answer

Some of the other causes of a fluctuating electrophoresis current error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Using Running Buffer that was:
-Diluted to 1X incorrectly
-Swapped with the water position on the Autosampler deck
-On the instrument longer than 48 hours
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5.Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, remove the syringe, gel block, anode and cathode buffer containers, water, and rinse containers and clean them with distilled water. Place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id:: E12683

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How do I perform a Clear Memory Reset? Product FAQ

Answer

The Clear Memory or two-button reset will clear the firmware image, memory, and all calibrated values from the CPU on the 310 Genetic Analyzer. Make sure that all of the calibrated values are known before doing this reset. Usually, these values can be found on a sticker on the inside of the front left door of the instrument.

To perform a Clear Memory reset:

(1) Turn off the instrument and then shut down the computer.

(2) Turn on the instrument while holding both the tray and gel buttons in for 10 seconds. The three lights (Green, Amber, and Red) should all be on, indicating that the firmware image and calibrated values have been erased.

(3) Turn on the computer. The 310 Data Collection should automatically launch and a window should appear that says "Sending Firmware image"(if the 310 Data Collection Software does not automatically launch, manually launch the program). Next, it will ask you to quit the Data Collection application and re-launch the software for this action (resending the Firmware) to take effect.

(4) After opening the 310 Data Collection Software again, open the Manual Control window and input all calibrated values that are listed on the sticker located inside the door (2 - CCD pixel positions, gel pump force, and syringe max travel values). Make sure to click on the "Execute" button after entering values to have them saved on the instrument.

(5) Recalibrate and home the AutoSampler and home the syringe.

Information on how to perform the Clear Memory Reset is also available in Revision B of the 310 Genetic Analyzer User Manual (Cat. No. 4317588), available through the Manuals and Protocols section of the Thermo Fisher Scientific Web site.

Answer Id:: E6263

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How do I turn on my Applied Biosystems 310 Genetic Analyzer? Product FAQ

Answer

The Applied Biosystems 310 Genetic Analyzer system includes both the instrument and the computer - you cannot start or restart one without starting the other. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The proper boot sequence is:

1. Remove the thermal tape securing the capillary closest to the electrode and pull the capillary up until the end of the capillary is about 1 mm below the end of the electrode. Failure to do so can cause the capillary to break when the instrument is turned on.
2. Start the computer and the instrument:
- Turn on the computer and log in, but do not launch the Data Collection software.
- Turn on the instrument and wait until you get a solid green light.
- Launch the Data Collection Software.
3. Re-calibrate the autosampler.
4. Home the syringe and refill with polymer if needed.

Step-by-step instructions for the start-up procedures can be found in the Applied Biosystems 310 Genetic Analyzer: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/310%20Start%20Up%20Procedures.pdf) and the Applied Biosystems 310 Genetic Analyzer User Guide (http://tools.thermofisher.com/content/sfs/manuals/cms_041158.pdf).

Answer Id:: E12664

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Application Note: Determination of Tetrabutylammonium and Tributylamine in Wastewater by IC/LC-MS Product Literature

Performance of in-line microfluidic mixers in laminar flow for high-throughput flow cytometry. Citations & References

  • Authors: Jackson WC, Bennett TA, Edwards BS, Prossnitz E, Lopez GP, Sklar LA
  • Journal: Biotechniques
  • PubMed ID: 12139249
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