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Bacteria Counting Kit Manual / Product Insert

  • Version: 01-04-2007
Catalog # B7277

Is the SYTO BC bacteria stain from the Bacteria Counting Kit available as a standalone product? Product FAQ

Answer

Yes, the SYTO BC bacteria stain can be purchased separately (Cat. No. S34855).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E9824

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Can I look at bacteria and yeast on a flow cytometer? Product FAQ

Answer

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14828

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When using the Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry, some cells seem to have both red and green signal. Are these cells dead or alive or dying? Product FAQ

Answer

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14916

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How can I count my bacteria by flow cytometry? Product FAQ

Answer

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14912

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I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do? Product FAQ

Answer

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14843

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I want to count my cells using flow cytometry. How do I do this? Product FAQ

Answer

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.

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Answer Id: E14822

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What bacterial parameters can I look at by flow cytometry? Product FAQ

Answer

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14852

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What is flow cytometry? Product FAQ

Answer

Cytometry is the measurement of physical or chemical characteristics of cells or particles. Flow cytometry measures these characteristics of cells or particles as they individually pass lasers in a flow cytometer instrument. Flow cytometry is performed on single cells, providing discrete measurements for each cell in the sample. It also provides a statistical distribution of the measured characteristics of the sample.

A flow cytometer is made up of three subsystems: fluidics, optics, and electronics. Fluidics moves the cells and introduces them for interrogation. Optics generates and collects the light signals. Electronics converts the optical signals to proportional electronic signals for computer analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14796

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Lot # 1637267 Certificate

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