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BacMam LRRK2 Reagent (Invitrogen™)

BacMam LRRK2 Wild Type Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length wild-type LRRK2 in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 Wild Type Reagent Lets You:

• Easily overexpress full-length LRRK2 in most cellular backgrounds
• Conduct the LRRK2 cellular assays you need
• Understand the effects of therapeutically relevant LRRK2 mutations

Examine LRRK2 Mutations with BacMam Reagents
Use therapeutic mutant reagents G2019S (Cat. no. A13389) and D1994A (Cat. no. A13390) along with the wild-type LRRK2 reagent to enable your research into LRRK2 mutations, including exploration of differences in cellular response to stimulus among different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function), and identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods, such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam LRRK2-GFP G2019S Reagent (Invitrogen™)

BacMam LRRK2-GFP G2019S Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length LRRK2-GFP G2019S in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2-GFP G2019S Reagent Lets You:

• Easily overexpress full-length LRRK2-GFP G2019S in most cellular backgrounds
• Visualize LRRK2 expression
• Conduct the LRRK2 cellular assays you need
• Understand the effects of therapeutically relevant LRRK2 mutations

Use BacMam Technology with GFP for Expression and Visualization of LRRK2
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2-GFP G2019S protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods, such as primary neurons and neuroblastoma cell lines.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2-GFP G2019S has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function) and identification of potential physiological substrates for LRRK2.

Examine LRRK2 Mutations with BacMam Reagents
Use therapeutic mutant LRRK2-GFP G2019S along with the wild-type LRRK2-GFP (Cat No A14170) reagent to enable your research into LRRK2 mutations, including exploration of differences in cellular response to stimulus among different mutations. Use LRRK2-GFP to confirm LRRK2-GFP protein expression and compare localization of wild type with pathological mutants in various cell backgrounds, especially primary neurons.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam LRRK2-GFP Wild Type Reagent (Invitrogen™)

BacMam LRRK2-GFP Wild Type Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length wild type LRRK2-GFP in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2-GFP Wild Type Reagent Lets You:

• Overexpress full-length LRRK2-GFP in most cellular backgrounds
• Visualize LRRK2 expression
• Conduct the LRRK2 cellular assays you need
• Understand the effects of therapeutically relevant LRRK2 mutations

Use BacMam Technology with GFP for Expression and Visualization of LRRK2
GFP (Green Fluorescent Protein) allows you to visually confirm LRRK2-GFP protein expression and compare localization of wild type with pathological mutants in various cellular backgrounds, especially primary neurons, while BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. BacMam enables efficient, titratable LRRK2-GFP protein expression in various mammalian cellular backgrounds including cell lines that may not be compatible with traditional transfection methods, such as primary neurons and neuroblastoma cell lines.

Expand Your Battery of Cellular Assays for LRRK2
Once full-length LRRK2-GFP has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function) and identification of potential physiological substrates for LRRK2.

Examine LRRK2 Mutations with BacMam Reagents
Use therapeutic mutant reagents G2019S (Cat No A14174) along with the wild type LRRK2-GFP reagent to enable your research into LRRK2 mutations, including exploration of differences in cellular response to stimulus among different mutations.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam LRRK2 D1994A Reagent (Invitrogen™)

BacMam LRRK2 D1994A Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length D1994A LRRK2 (kinase-dead LRRK2 mutant) in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 D1994A Reagent Lets You:

• Investigate LRRK2 mutation mechanisms and stimulus responses
• Assay LRRK2 relevance across a broad range of parameters
• Utilize most mammalian cellular backgrounds for LRRK2 overexpression

Examine LRRK2 Mutations with BacMam Reagents
Use of the BacMam LRRK2 therapeutic mutant reagents G2019S (Cat. no. A13389) and D1994A in addition to the wild-type LRRK2 reagent (Cat. no. A13388) enables you to research and determine LRRK2 mutant mechanisms. You can also evaluate the differences in cellular response to a stimulus among the different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 D1994A has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 research. Some examples include studying the regulation of physiologically functional pathways (including signaling pathways leading to function), or identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 D1994A protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam LRRK2 G2019S Reagent (Invitrogen™)

BacMam LRRK2 G2019S Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length G2019S LRRK2 upregulating mutation in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 G2019S Reagent Lets You:

• Easily overexpress full-length LRRK2 G2019S in most cellular backgrounds
• Understand the effects of therapeutically relevant LRRK2 mutations
• Conduct the LRRK2 cellular assays you need

Examine LRRK2 Mutations with BacMam Reagents
Using the BacMam LRRK2 therapeutic mutant reagents G2019S and D1994A (Cat. no. A13390) along with the wild-type LRRK2 reagent (Cat. no. A13388) enables you to research and determine LRRK2 mutant mechanisms. You can also evaluate the difference in the cellular response to a stimulus among the different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 G2019S has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function), and identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 G2019S protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

LanthaScreen™ Tb-anti-LRRK2 [pSer935] Antibody

The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody enables researchers to interrogate phosphorylation at serine 935 of the Leucine-Rich Repeat Kinase-2 (LRRK2) protein in a cellular, high-throughput screening (HTS)-compatible, and TR-FRET format.

This antibody has been specifically developed and optimized for use in our LanthaScreen® TR-FRET LRRK2 Cellular Activity Assay Kits (available for both wildtype and disease relevant mutant protein), which utilize the BacMam gene delivery system. The antibody, as well as other components of these assay kits, are also available separately. This assay format provides a convenient tool for the expression of LRRK2-GFP fusion protein in the cell background of your choice. The resulting cellular assay can be used to identify LRRK2 and relevant mutant inhibitor.

This product includes:

• LanthaScreen® Tb-anti-LRRK2 [pSer935] Antibody, 1 mg (enough to perform 275 384-well plate assays)

Additional products needed for cell-based assay:

BacMam LRRK2-GFP Wild Type Reagent, 15 mL or BacMam LRRK2-GFP G2019S Reagent, 15 mL
LanthaScreen® 6x Lysis Buffer, 50 mL
Instrument Control Terbium TR-FRET Kit

Benefits of this assay include:

More Physiologically Relevant: cell-based LRRK2 kinase activity and choice of cell background
More Convenient: compatible with High Throughput Screening (HTS); LanthaScreen® technology reduces interference and noise
Disease Relevant: screen compound libraries for Parkinson's disease-relevant molecules

More Physiologically Relevant
The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody allows the investigation of phosphorylation at serine 935 on LRRK2 protein kinase. When paired with LRRK2 BacMam reagents, you have the freedom to choose the cellular background for your assay, including primary cells. This enables screening for potential inhibitors of LRRK2 proteins in their natural complexes in a physiologically relevant cell type.

More Convenient
Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as western blotting and ELISA, making this assay ideal for HTS applications. The LanthaScreen® technology provides all of the advantages of TR-FRET ratiometric detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, which results in the use of fewer cells than traditional Western or ELISA methods.

Disease Relevant
The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody can be used by Parkinson's and other disease researchers to perform HTS, cell-based assays to generate inhibition curves (IC50) for their compound libraries. This will aid drug discovery efforts by reducing the time to results from 4 hrs to typically 1.5 hrs compared to alternative ELISAs.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology