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          Documents & Support

          Biochemical Reagents SDS

          Catalog # OXDBRS-CLP1-OXD

          Biochemical Reagents SDS

          Catalog # AUBRS

          Brochure: Alfa Aesar Capabilities Product Literature

          Waterbook [EN] Product Literature

          Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Citations & References

          • Authors: Szoka F, Papahadjopoulos D
          • Journal: Proc Natl Acad Sci U S A (1978) 75:4194-4198
          • PubMed ID: 279908
          Catalog #
          • C1359
          • C1360
          • C1904(Discontinued)
          • C194(Discontinued)

          Selective chemical treatment of cellular microdomains using multiple laminar streams. Citations & References

          • Authors: Takayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM
          • Journal: Chem Biol (2003) 10:123-130
          • PubMed ID: 12618184
          Catalog #

          Microbiology Product Catalog - Europe Product Literature

          I accidentally stored Cellfectin II Reagent (Cat. No. 10362100) at -20 degrees C. Can I still use it? Product FAQ

          Answer

          We can't guarantee performance because freezing compromises activity of the reagent.

          Answer Id:: E19203

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          May I filter-sterilize the siRNA/lipid complex before injection into the mouse? Product FAQ

          Answer

          Yes, you may do so by using a 0.22 µm pore size for filter-sterilization. However, our best recommendation is to always keep your procedure sterile from start-to-finish.

          Answer Id:: E9059

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          Can I co-transfect plasmid and Cas9 protein (RNP) with Lipofectamine Stem Transfection Reagent? Product FAQ

          Answer

          Yes. Lipofectamine Stem Transfection Reagent can be used to co-deliver multiple payloads, including DNA, mRNA, and Cas9 protein (RNP) into stem cells.

          Answer Id:: E15215

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          How do I isolate RNA from liver or other organs for in vivo delivery? Product FAQ

          Answer

          Isolating high-quality RNA is critical to the overall success of in vivo experiments. Please visit the Invitrogen RNA Purification page for how to best handle and isolate RNA from in vivo samples: https://www.thermofisher.com/us/en/home/brands/invitrogen/ambion.html

          Answer Id:: E9060

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          With Lipofectamine Stem Transfection Reagent, how can I improve the transfection efficiency in my stem cells? Product FAQ

          Answer

          To obtain the highest percent of stem cells transfected with Lipofectamine Stem Transfection Reagent, we highly recommend that you follow cell culture conditions that promote formation of a monolayer versus clumps of cells. If the density of your stem cells is greater than 50% or they are being grown as colonies in other media, more Lipofectamine Stem Transfection Reagent may improve transfection efficiency. For additional details, please refer to the Lipofectamine Stem Transfection Reagent protocol pertinent to your cell type and media on the product page (https://www.thermofisher.com/order/catalog/product/STEM00008?ICID=search-product), under Documents, Product Literature.

          Answer Id:: E15216

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          How does the use of Lipofectamine 2000 reagent improve transfection over the other lipid reagents? Product FAQ

          Answer

          It generally yields better transfection activity (as measured by protein expression) than all other lipids in a majority of the cell lines tested. It includes a streamlined, simple protocol where the complexes are added directly to cells without changing media. This lends itself to high throughput applications. It works very well in the presence or absence of serum. Examples of cells that show the highest transfection efficiency with Lipofectamine 2000 include 293 F, 293 H, BE(2)C, CHO-K1, CHO-S, COS-1, COS7-L, Human Primary Fibroblasts, Ht-29, HT-1080, MDCK, MRC-5, PC12 and Vero.

          Answer Id:: E3162

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          For in vivo siRNA delivery, do I need to weigh my mouse to determine dosing, or can I use the standard 200 µL of complexed material for systemic intravenous injection? Product FAQ

          Answer

          To ensure the best results and minimal toxicity, it is very important to determine the weight of each mouse to provide the correct dose and to maintain uniform dosing of multiple animals. The recommended dose for mice is 1.0 mg/kg (intravenous), depending on the potency of your siRNA.

          Answer Id:: E9051

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          Do you have any suggestions for genes to use for normalizing my qPCR analysis after in vivo siRNA delivery? Product FAQ

          Answer

          We recommend using housekeeping genes for normalization. The expression levels of housekeeping genes can vary depending on the experimental conditions and/or tissue type. We have obtained the most consistent results by normalizing target gene expression to HMBS and GAPDH. Consider using the TaqMan Array Mouse or Rat Endogenous Control Panels to identify appropriate controls regardless of cell, tissue, or organ combined with treatment type. To look for endogenous controls in other species, please visit our TaqMan Endogenous Control Assays here https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/taqman-endogenous-controls.html

          For additional information on housekeeping genes, please visit our qPCR Education webpage: https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education.html

          Answer Id:: E9061

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