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CASP3 Recombinant Human Protein, active (Gibco™)

Recombinant CASP3 (caspase 3, apoptosis-related cysteine peptidase)) is a bioactive protein intended for use in cell culture applications. Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. This protein cleaves and activates caspases 6, 7, and 9. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein.

Caspase 3 Monoclonal Antibody (74T2) (Invitrogen™)

Caspase 3 Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared (Invitrogen™)

Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared for ELISA

Caspase 3 (Cleaved) Human ELISA Kit (Invitrogen™)

Caspase 3 (Cleaved) Human ELISA Kit for ELISA

Caspase 3 Monoclonal Antibody (4-1-18) (Invitrogen™)

Caspase 3 Monoclonal Antibody for Western Blot, IHC

Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric (Invitrogen™)

Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Colorimetric for ELISA

Caspase 3 (Total) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Caspase-3 Simplex ProcartaPlex kit measures caspase-3 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Caspase 3 Antibody (9H19L2), ABfinity™ Rabbit Monoclonal (Invitrogen™)

Caspase 3 Monoclonal Antibody for Western Blot, IF, ICC, IHC (P)

Caspase 3 Human Instant ELISA™ Kit (Invitrogen™)

Caspase 3 Human Instant ELISA™ Kit for ELISA

Caspase 3 Antibody (22HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

Caspase 3 Oligoclonal Antibody for Western Blot, IF, ICC

CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen™)

The CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit contains the reagents to sensitively detect active Caspase-3 in mammalian cells. This assay utilizes the inhibitor specific for Caspase-3, DEVD-FMK, that is directly conjugated to FITC (fluorescein isothiocyanate) for the detection system. This reagent is cell permeable, non-toxic and irreversibly binds to the active enzyme. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

The Caspases are a family of aspartate-specific cysteine proteases that act in a step-wise signaling manner like kinases. Recruitment of these proteases to oligomerized receptors leads to activation and autoproteolytic cleavage. Active caspases can proteolyze additional caspases generating a caspase cascade. The final outcome of this signaling pathway is a form of controlled cell death, termed apoptosis.

Caspase-3, also known as Yama, CPP32, and apopain, cleaves its substrates at the carboxyl terminus of aspartate residues. Active Caspase-3 consists of 2 sets of homodimers (~ 17 and 12 kDa) that are derived from two precursor Caspase-3 polypeptides and has two active sites. Caspase-3 is proteolytically activated by other caspases.

Both subunits contribute to substrate binding and catalysis. The active site cysteine that covalently binds the substrate is located near the C-terminus of the large subunit. Active Caspase-3 has two-fold symmetry, two active site pockets each residing on an opposite side. Caspase-3, together with Caspases 8 and 9, is situated at pivotal junctions in apoptotic pathways. Caspase-3 appears to amplify Caspase 8 and 9 initiation signals into complete commitment to apoptotic disassembly.

Reported Application
Flow Cytometric Analysis, Immunocytochemistry

Caspase 3 Polyclonal Antibody, Biotin (Invitrogen™)

Caspase 3 Polyclonal Antibody for Western Blot, IHC (P), IP

Caspase 3 Monoclonal Antibody (3CSP03 (4.1.18)) (Invitrogen™)

Caspase 3 Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

Caspase 3 Polyclonal Antibody (Invitrogen™)

Caspase 3 Polyclonal Antibody for IHC

Caspase 3 Monoclonal Antibody (3CSP01 (7.1.44)) (Invitrogen™)

Caspase 3 Monoclonal Antibody for Western Blot, IHC (P), IP