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Sca-1 Recombinant Polyclonal Antibody (7 HCLC) (Invitrogen™)

Sca-1 Recombinant Polyclonal Antibody for Western Blot, IF, ICC, IHC

Lab Vision™ Caspase 6 (Mch 2) Ab-3, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in western blotting and immunoprecipitation procedures with Thermo Scientific™ Caspase 6 (Mch 2) Ab-3, Mouse Monoclonal Antibody.

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Lab Vision™ Caspase 3 (CPP32) Ab-4, Rabbit Polyclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation procedures.

CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit (Invitrogen™)

The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit contains all the reagents necessary to detect active caspase-8 in cells with high sensitivity. Fluorescein (FITC)-conjugated IETD-FMK, a specific inhibitor of caspase-8, is utilized in this assay for detection. This reagent is cell permeable, non-toxic and irreversibly binds to the active enzyme. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

The caspases constitute a family of aspartate-specific cysteine proteases that mediate a sequence of cleavage events. Recruitment of the inactive proenzyme to oligomerized receptors leads to caspase activation and autoproteolytic cleavage. These active enzymes can then cleave other caspases, thereby generating a caspase signaling cascade that leads to a form of programmed cell death termed apoptosis.

Caspase-8, which is also known as FLICE, MACHalpha1, and Mch5, cleaves its substrates at the C-terminal aspartic acid residue of the motif Asp-X-X-Asp. Eight isoforms of caspase-8 exist, of which caspase-8/a and 8/b are the predominant forms. Upon stimulation of death receptors such as CD95/APO-1/Fas, TRAIL-R1, TRAIL-R2, TNFR1, and TRAMP, caspase-8 is recruited to the death-inducing signaling complex (DISC). Subsequent dimerization leads to caspase-8 activation via autocatalytic cleavage, which leads to the formation of a 12-kDa prodomain and a 43-kDa intermediate fragment that is further cleaved to produce 26-kDa and 18-kDa active enzymes. As an initiator caspase, this enzyme initiates a caspase signaling cascade that results in apoptosis. Substrates of caspase-8 include caspases-3 and -7, as well as the pro-apoptotic Bcl-2 family member Bid. In addition to its role in cell death, caspase-8 has been linked to cell adhesion and motility.

Reported Application
Flow Cytometric Analysis, Immunocytochemistry

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), FITC, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Lab Vision™ Caspase 8 (FLICE) Ab-3, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments with Thermo Scientific™ Caspase 8 (FLICE) Ab-3, Mouse Monoclonal Antibody.

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), APC, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Caspase 9 Polyclonal Antibody, eBioscience™ (Invitrogen™)

Caspase 9 Polyclonal Antibody for Western Blot, IP

Caspase 1 Monoclonal Antibody (5B10), eBioscience™ (Invitrogen™)

Caspase 1 Monoclonal Antibody for Western Blot

CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit (Invitrogen™)

The CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit contains all the reagents necessary to detect active caspase-9 in mammalian cells with high sensitivity. Fluorescein (FITC)-conjugated LEHD-FMK, a specific inhibitor of caspase-9, is utilized in this assay for detection. This reagent is cell permeable, non-toxic and irreversibly binds to the active enzyme. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

The caspases constitute a family of aspartate-specific cysteine proteases that mediate a sequence of cleavage events. Recruitment of the inactive proenzyme to oligomerized receptors leads to caspase activation and autoproteolytic cleavage. These active enzymes can then cleave other caspases, thereby generating a caspase signaling cascade that leads to a form of programmed cell death termed apoptosis.

Caspase-9, which is also known as ICE-LAP6 and Mch6, cleaves its substrates at the C-terminal aspartic acid residue of the motif Asp-X-X-Asp. Activation of this signaling protease occurs upon stimulation of death receptors such as CD95/APO-1/Fas, TRAIL-R1, TRAIL-R2, TNFR1, and TRAMP. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis. Substrates of caspase-9 include caspases-3, -6, and -7. Finally, dimerization of cleaved caspase-9 is inhibited by X-linked inhibitor of apoptosis (XIAP).

Reported Application
Flow Cytometric Analysis, Immunocytochemistry

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine5, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Alexa Fluor 700, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Biotin, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 436, eBioscience™ (Invitrogen™)

Ly-6A/E (Sca-1) Monoclonal Antibody for Flow