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Phospho-Caspase 8 (Ser387) Antibody (10HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

Phospho-Caspase 8 (Ser387) Oligoclonal Antibody for Western Blot, IF, ICC

Caspase 8 Human ELISA Kit (Invitrogen™)

Caspase 8/FLICE Human ELISA Kit for ELISA

CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit (Invitrogen™)

The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit contains all the reagents necessary to detect active caspase-8 in cells with high sensitivity. Fluorescein (FITC)-conjugated IETD-FMK, a specific inhibitor of caspase-8, is utilized in this assay for detection. This reagent is cell permeable, non-toxic and irreversibly binds to the active enzyme. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

The caspases constitute a family of aspartate-specific cysteine proteases that mediate a sequence of cleavage events. Recruitment of the inactive proenzyme to oligomerized receptors leads to caspase activation and autoproteolytic cleavage. These active enzymes can then cleave other caspases, thereby generating a caspase signaling cascade that leads to a form of programmed cell death termed apoptosis.

Caspase-8, which is also known as FLICE, MACHalpha1, and Mch5, cleaves its substrates at the C-terminal aspartic acid residue of the motif Asp-X-X-Asp. Eight isoforms of caspase-8 exist, of which caspase-8/a and 8/b are the predominant forms. Upon stimulation of death receptors such as CD95/APO-1/Fas, TRAIL-R1, TRAIL-R2, TNFR1, and TRAMP, caspase-8 is recruited to the death-inducing signaling complex (DISC). Subsequent dimerization leads to caspase-8 activation via autocatalytic cleavage, which leads to the formation of a 12-kDa prodomain and a 43-kDa intermediate fragment that is further cleaved to produce 26-kDa and 18-kDa active enzymes. As an initiator caspase, this enzyme initiates a caspase signaling cascade that results in apoptosis. Substrates of caspase-8 include caspases-3 and -7, as well as the pro-apoptotic Bcl-2 family member Bid. In addition to its role in cell death, caspase-8 has been linked to cell adhesion and motility.

Reported Application
Flow Cytometric Analysis, Immunocytochemistry

Caspase 8 Monoclonal Antibody (8CSP03 (4.1.20)) (Invitrogen™)

Caspase 8 Monoclonal Antibody for Western Blot, IP

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for Western Blot, IF, ICC

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for Western Blot

FLASH Polyclonal Antibody (Invitrogen™)

FLASH Polyclonal Antibody for Western Blot, IF, ICC

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for IHC (P)

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for IHC (P)

Phospho-Caspase 8 (Ser347) Polyclonal Antibody (Invitrogen™)

Phospho-Caspase 8 (Ser347) Polyclonal Antibody for Western Blot, IHC (P)

Caspase 8 Monoclonal Antibody (1H11) (Invitrogen™)

Caspase 8 Monoclonal Antibody for Western Blot, IHC, Flow

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for Western Blot, IF, ICC, IHC, Flow

Caspase 8 Monoclonal Antibody (FLICE 4-1-20) (Invitrogen™)

Caspase 8 Monoclonal Antibody for Western Blot

Caspase 8 Monoclonal Antibody (B.925.8) (Invitrogen™)

Caspase 8 Monoclonal Antibody for Western Blot, IP

Caspase 8 Polyclonal Antibody (Invitrogen™)

Caspase 8 Polyclonal Antibody for IHC (F), IHC (P)