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RANTES Recombinant Human Protein (Gibco™)

Recombinant RANTES is a bioactive protein intended for use in cell culture applications. RANTES functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. RANTES is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.

RANTES Rat ELISA Kit (Invitrogen™)

RANTES Rat ELISA Kit for ELISA

RANTES Mouse Instant ELISA™ Kit (Invitrogen™)

RANTES Mouse Instant ELISA™ Kit for ELISA

RANTES Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human RANTES Simplex ProcartaPlex kit measures RANTES protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

RANTES Feline ELISA Kit (Invitrogen™)

RANTES Feline ELISA Kit for ELISA

CCL5 (RANTES) Monoclonal Antibody (947A 1H11), Biotin (Invitrogen™)

CCL5 (RANTES) Monoclonal Antibody for ELISA

RANTES Monoclonal Antibody (947A5G6) (Invitrogen™)

CCL5 (RANTES) Monoclonal Antibody for ELISA

CCL5 (RANTES) Polyclonal Antibody (Invitrogen™)

CCL5 (RANTES) Polyclonal Antibody for Western Blot, IHC (P), ELISA, FN

CCL5 (RANTES) Polyclonal Antibody (Invitrogen™)

CCL5 (RANTES) Polyclonal Antibody for Western Blot

CCL5 (RANTES) Polyclonal Antibody (Invitrogen™)

CCL5 (RANTES) Polyclonal Antibody for Western Blot, ELISA

CCL5 (RANTES) Polyclonal Antibody (Invitrogen™)

CCL5 (RANTES) Polyclonal Antibody for Western Blot

CCL5 (RANTES) Monoclonal Antibody (53405.111) (Invitrogen™)

CCL5 (RANTES) Monoclonal Antibody for IF, ICC, IA

CCL5 (RANTES) Polyclonal Antibody (Invitrogen™)

CCL5 (RANTES) Polyclonal Antibody for ELISA

Human 42-Plex Human ProcartaPlex™ Panel 1, Platinum (Invitrogen™)

Platinum ProcartaPlex™ Immunoassays are our are highest validated bead based Multiplexing assay system for quantificaton of multiple protein biomarkers in a single sample.
Newly developed matrix type specific diluents for plasma and serum enable to overcome matrix effects and assure high performance specifications (e.g spike and dilution recovery results between 70-130%).
Our Platinum ProcartaPlex assays use Luminex™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins. Platinum ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA assays.

Platinum ProcartaPlex Panels are ready-to-use kits and are not combinable with other Platinum ProcartaPlex Simplex kits or kits from different format.

Reactivity/Species
Human

Suitable Sample Types
Serum, Plasma

Sample Volume
Serum, Plasma: 25 µL

Reported Application
Multiplex Immunoassay

Target List
IL-12; Brain-Derived Neurotrophic Factor (BDNF); CCL11 (Eotaxin); CCL3 (MIP-1 alpha); CCL4 (MIP-1 beta); CCL5 (RANTES); CD31 (PECAM-1); CD62P (P-Selectin); CXCL1 (GRO alpha); CXCL10 (IP-10); GM-CSF; HGF; IFN alpha; IFN gamma; IL-1 alpha; IL-1 beta; IL-1 Receptor Antagonist; IL-10; IL-13; IL-15; IL-16; IL-17A; IL-2; IL-20; IL-21; IL-4; IL-5; IL-6; IL-7; IL-8 (CXCL8); IL-9; LIF; OPG; sCD120b (sTNF Receptor II); Stem Cell Factor (SCF); TNF alpha; t-PA; TSLP; VEGF-A; VEGF-D; PDGF-BB; MCP-2

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

TARC Recombinant Rabbit Monoclonal Antibody (B22H33L5) (Invitrogen™)

TARC Recombinant Monoclonal Antibody for Western Blot, IHC (P)