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CCL3 (MIP-1 alpha) Human Uncoated ELISA Kit with Plates (Invitrogen™)

CCL3 (MIP-1 alpha) Human Uncoated ELISA Kit with Plates for ELISA

Cytokine & Chemokine 36-Plex Mouse ProcartaPlex™ Panel 1A (Invitrogen™)

The Mouse Cytokine & Chemokine IA 36-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 36 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin; IFN alpha; IL-15/IL-15R; IL-28; IL-31; IL-1 alpha; IL-3; G-CSF; LIF; ENA-78/CXCL5; M-CSF

Bead Regions
IFN gamma: 38; IL-12p70: 39; IL-13: 35; IL-1 beta: 19; IL-2: 20; IL-4: 26; IL-5: 27; IL-6: 28; TNF alpha: 45; GM-CSF: 42; IL-18: 66; IL-10: 13; IL-17A: 52 ; IL-22: 33; IL-23: 37; IL-27: 36; IL-9: 34; GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62; IFN alpha: 30; IL-15/IL-15R: 54; IL-28: 64; IL-3: 14; G-SCF: 12; M-CSF: 21; LIF: 18; IL-1 alpha: 56; ENA-78: 57; IL-31: 76

Assay Range
See product documentation

Assay Sensitivity
See product documentation

CCL3 (MIP-1 alpha) Human Uncoated ELISA Kit (Invitrogen™)

CCL3 (MIP-1 alpha) Human Uncoated ELISA Kit for ELISA

Eotaxin Recombinant Human Protein (Gibco™)

Recombinant human eotaxin is a bioactive protein intended for use in cell culture applications. Eotaxin is a member of the PF-4 family of chemokines. Eotaxin plays a role in inducing calcium flux in eosinophils.

MCP-2 Recombinant Mouse Protein (Gibco™)

Recombinant MCP-2 is a bioactive protein intended for use in cell culture applications. MCP-2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils.

Chemokine 8-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Chemokine 8-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 8 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-2; Rantes

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] Eotaxin: 1.9-7700 pg/mL; GRO alpha: 11.4-46500 pg/mL; IP-10: 4.9-20100 pg/mL; MCP-1: 18.03-75000 pg/mL; MCP-3: 3.5-14200 pg/mL; MIP-1 alpha: 2.3-9500 pg/mL; MIP-2: 0.6-2400 pg/mL; Rantes: 85.5-350000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] Eotaxin: 1.1 pg/mL; GRO alpha: 1.4 pg/mL; IP-10: 0.9 pg/mL; MCP-1: 10.9 pg/mL; MCP-3: 1.1 pg/mL; MIP-1 alpha: 1.0 pg/mL; MIP-2: 0.1 pg/mL; Rantes: 6.1 pg/mL

Cytokine & Chemokine Convenience 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin/CCL11; GM-CSF; GRO alpha/CXCL1; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF1 alpha/CXCL12; TNF alpha; TNF beta/LTA

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th1/Th2 Cytokine & Chemokine 20-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Th1/Th2 Cytokine & Chemokine 20-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 20 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GM-CSF; IFN gamma; IL-1 beta; IL-2; IL-4; IL-5; IL-6; IL-12 p70; IL-13; IL-18; TNF alpha; Eotaxin; GRO-alpha; IL-8; IP-10; MCP-1; MIP-1 alpha; MIP-1 beta; Rantes; SDF-1 alpha

Bead Regions
IFN-gamma: 43; IL-12p70: 34; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF-alpha: 45; GM-CSF: 44; IL-18: 66; Eotaxin: 33; GRO-alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; Rantes: 42; SDF-1 alpha: 13

Assay Range
[As determined for Lot 1:] IFN gamma: 12.21-50000 pg/mL; IL-12p70: 6.84-28000 pg/mL; IL-13: 2.44-10000 pg/mL; IL-1 beta: 2.44-10000 pg/mL; IL-2: 4.88-20000 pg/mL; IL-4: 12.21-50000 pg/mL; IL-5: 7.32-30000 pg/mL; IL-6: 9.77-40000 pg/mL; TNF-alpha: 8.54-35000 pg/mL; GM-SCF: 17.09-70000; IL-18: 9.77-40000 pg/mLAs; Eotaxin: 0.61-2500 pg/mL; GRO-alpha: 2.44-10000 pg/mL; IL-8: 2.44-10000 pg/mL; IP-10: 1.95-8000 pg/mL; MCP-1: 1.22-5000 pg/mL; MIP-1 alpha: 2.14-8750 pg/mL; MIP-1 beta: 6.10-25000 pg/mL; Rantes: 0.61-2500 pg/mL; SDF1-alpha: 17.09-70000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.2 pg/mL; IL-12p70: 0.04 pg/mL; IL-13: 0.1 pg/ml; IL-1 beta: 0.2 pg/mL; IL-2: 0.8 pg/mL; IL-4: 1.5 pg/mL; IL-5: 0.3 pg/mL; IL-6: 0.4 pg/mL; TNF-alpha: 0.4 pg/mL; GM-CSF: 1.2 pg/mL; IL-18: 0.4 pg/mL; Eotaxin: 1.4 pg/mL; GRO-alpha: 2.8 pg/mL; IL-8: 1.2 pg/mL; IP-10: 0.3 pg/mL; MCP-1: 0.6 pg/mL; MIP-1 alpha: 1.1 pg/mL; MIP-1 beta: 4.7 pg/mL; Rantes: 0.2 pg/mL; SDF1-alpha: 20.5 pg/mL

Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen™)

The Mouse Cytokine & Chemokine 26-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 26 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
IFN gamma: 38; IL-12p70: 39; IL-13: 35; IL-1 beta: 19; IL-2: 20; IL-4: 26; IL-5: 27; IL-6: 28; TNF alpha: 45; GM-CSF: 42; IL-18: 66; IL-10: 13; IL-17A: 52 ; IL-22: 33; IL-23: 37; IL-27: 36; IL-9: 34; GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th9/Th17/Th22/Treg Cytokine 15-Plex Mouse ProcartaPlex™ Panel (Invitrogen™)

The Mouse Th9/Th17/Th22/Treg Cytokine 15-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 15 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
IL-10: 13; IL-17A: 52 ; IL-22: 33; IL-23: 37; IL-27: 36; IL-9: 34; GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62

Assay Range
[As determined for Lot 1:] IL-10: 2.4-10000 pg/mL; IL-17A: 1.2-5000 pg/mL; IL-22: 12.2-50000 pg/mL; IL-23: 12.2-50000 pg/mL; IL-27: 2.4-10000 pg/mL; IL-9: 18.3-75000 pg/mL; Gro alpha: 1.7-7000 pg/mL; IP-10: 0.5-2000 pg/mL; MCP-1: 7.3-30000 pg/mL; MCP-3: 0.2-1000 pg/mL; MIP-1 alpha: 0.5-2000 pg/mL; MIP-1 beta: 1.2-5000 pg/mL; MIP-2: 0.7-3000 pg/mL; RANTES: 2.4-10000 pg/mL; Eotaxin: 0.5-2000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-10: 0.69 pg/mL; IL-17A: 0.08 pg/mL; IL-22: 0.24 pg/mL; IL-23: 2.21 pg/mL; IL-27: 0.34 pg/mL; IL-9: 0.28 pg/mL; Gro alpha: 0.05 pg/mL; IP-10: 0.26 pg/mL; MCP-1: 3.43 pg/mL; MCP-3: 0.15 pg/mL; MIP-1 alpha: 0.13 pg/mL; MIP-1 beta: 1.16 pg/mL; MIP-2: 0.37 pg/mL; RANTES: 0.35 pg/mL; Eotaxin: 0.01 pg/mL

MCP-5 Mouse Instant ELISA™ Kit (Invitrogen™)

MCP-5 Mouse Instant ELISA™ Kit for ELISA

Eotaxin-2 Recombinant Mouse Protein (Gibco™)

Recombinant mouse eotaxin is a bioactive protein intended for use in cell culture applications. Eotaxin is a member of the PF-4 family of chemokines. Eotaxin plays a role in inducing calcium flux in eosinophils.

CCL4 Human Uncoated ELISA Kit with Plates (Invitrogen™)

This Human CCL4 (MIP-1 beta) Uncoated ELISA Kit with Plates contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of protein levels from samples including serum, plasma, and supernatants from cell cultures.

CCL4, also known as MIP-1 beta (Macrophage Inflammatory Protein-1 beta), is a member of the CC-subfamily of chemokines and is most closely related to CCL3, or MIP-1 alpha. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 are chemoattractant for monocytes, macrophages, and dendritic cells, CCL4 preferentially attracts CD4+ T cells, while CD8+ T cells are more responsive to CCL3. In addition to its chemotactic functions, CCL4 induces the release of proinflammatory cytokine, mast cells degranulation, and NK cell activation.

CCL4 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL3 and CCL5 (RANTES). CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.

Reported Application
ELISA

Chemokine 9-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen™)

The Mouse Chemokine I 9-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 9 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62

Assay Range
[As determined for Lot 1:] Gro alpha: 1.7-7000 pg/mL; IP-10: 0.5-2000 pg/mL; MCP-1: 7.3-30000 pg/mL; MCP-3: 0.2-1000 pg/mL; MIP-1 alpha: 0.5-2000 pg/mL; MIP-1 beta: 1.2-5000 pg/mL; MIP-2: 0.7-3000 pg/mL; RANTES: 2.4-10000 pg/mL; Eotaxin: 0.5-2000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] Gro alpha: 0.05 pg/mL; IP-10: 0.26 pg/mL; MCP-1: 3.43 pg/mL; MCP-3: 0.15 pg/mL; MIP-1 alpha: 0.13 pg/mL; MIP-1 beta: 1.16 pg/mL; MIP-2: 0.37 pg/mL; RANTES: 0.35 pg/mL; Eotaxin: 0.01 pg/mL

6Ckine Recombinant Mouse Protein (Gibco™)

Recombinant 6Ckine is a bioactive protein intended for use in cell culture applications. Recombinant mouse 6Ckine is intended for use in cell culture applications. 6Ckine is a chemokine involved in chemotaxis of specific cell types.