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MIP-1a (CCL3) Human Instant ELISA™ Kit (Invitrogen™)

MIP-1a (CCL3) Human Instant ELISA™ Kit for ELISA

MCP-2 Recombinant Mouse Protein (Gibco™)

Recombinant MCP-2 is a bioactive protein intended for use in cell culture applications. MCP-2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils.

MCP-1 Human ELISA Kit (Invitrogen™)

MCP-1 Human ELISA Kit for ELISA

Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Cytokine & Chemokine & Growth Factor 45-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 45 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
BDNF; Eotaxin/CCL11; EGF; FGF-2; GM-CSF; GRO alpha/CXCL1; HGF; NGF beta; LIF; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF-1 alpha/CXCL12; TNF alpha; TNF beta/LTA; PDGF-BB; PLGF; SCF; VEGF-A; VEGF-D

Bead Regions
IFN gamma: 43; IL-12p70: 34 ; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF alpha: 45; GM-CSF: 44; IL-18: 66; IL-10: 28; IL-17A: 36; IL-21: 72; IL-22: 76; IL-23: 63; IL-27: 14; IL-9: 52; IFN alpha: 48; IL-31: 37; IL-15: 65; IL-1 alpha: 62; IL-1RA: 38; IL-7: 26; TNF beta: 54; Eotaxin: 33; GRO alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; RANTES: 42; SDF-1 alpha: 13; NGF beta: 55; EGF: 56; FGF-2: 75; HGF: 46; BDNF: 57; PDGF-BB: 77; PIGF-1: 29; SCF: 39; VEGF-A: 78; LIF: 15; VEGF-D: 53

Assay Range
See product documentation

Assay Sensitivity
See product documentation

MCP-5 Mouse Instant ELISA™ Kit (Invitrogen™)

MCP-5 Mouse Instant ELISA™ Kit for ELISA

CCL4 (MIP-1 beta) Human Uncoated ELISA Kit (Invitrogen™)

CCL4 (MIP-1 beta) Human Uncoated ELISA Kit for ELISA

MCP-1/CCL2 Mouse Uncoated ELISA Kit (Invitrogen™)

MCP-1/CCL2 Mouse Uncoated ELISA Kit for ELISA

Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

MCP-1 Rat Instant ELISA™ Kit (Invitrogen™)

MCP-1 Rat Instant ELISA™ Kit for ELISA

Cytokine & Chemokine Convenience 36-Plex Mouse ProcartaPlex™ Panel 1A (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin; IFN alpha; IL-15/IL-15R; IL-28; IL-31; IL-1 alpha; IL-3; G-CSF; LIF; ENA-78/CXCL5; M-CSF

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th9/Th17/Th22 Cytokine 16-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Th9/Th17/Th22 Cytokine I 16-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 16 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-9; IL-10; IL-17A; IL-21; IL-22; IL-23; IL-27; Eotaxin; GRO-alpha; IL-8; IP-10; MCP-1; MIP-1 alpha; MIP-1 beta; RANTES; SDF-1 alpha

Bead Regions
IL-10: 28; IL-17A: 36; IL-21: 72; IL-22: 76; IL-23: 63; IL-27: 14; IL-9: 52; Eotaxin: 33; GRO-alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; RANTES: 42; SDF-1 alpha: 13

Assay Range
[As determined for Lot 1:] IL-10: 2.44-10000 pg/mL; IL 17A: 2.44-10000 pg/mL; IL-21: 9.77-40000 pg/mL; IL-22: 31.74-130000 pg/mL; IL-23: 14.65-60000 pg/mL; IL-27: 24.41-100000 pg/mL; IL-9: 9.77-40000 pg/mL; Eotaxin: 0.61-2500 pg/mL; GRO-alpha: 2.44-10000 pg/mL; IL-8: 2.44-10000 pg/mL; IP-10: 1.95-8000 pg/mL; MCP-1: 1.22-5000 pg/mL; MIP-1 alpha: 2.14-8750 pg/mL; MIP-1 beta: 6.10-25000 pg/mL; RANTES: 0.61-2500 pg/mL; SDF1-alpha: 17.09-70000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-10: 0.1 pg/mL; IL-17A: 0.1 pg/mL; IL-21: 0.6 pg/mL; IL-22: 8.2 pg/mL; IL-23: 0.9 pg/mL; IL-27: 5.1 pg/mL; IL-9: 0.5 pg; Eotaxin: 1.4 pg/mL; GRO-alpha: 2.8 pg/mL; IL-8: 1.2 pg/mL; IP-10: 0.3 pg/mL; MCP-1: 0.6 pg/mL; MIP-1 alpha: 1.1 pg/mL; MIP-1 beta: 4.7 pg/mL; RANTES: 0.2 pg/mL; SDF1-alpha: 20.5 pg/mL

Chemokine 9-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Chemokine I 9-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 9 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin; GRO alpha; IL-8; IP-10; MCP-1; MIP-1 alpha; MIP-1 beta; SDF-1 alpha; RANTES

Bead Regions
Eotaxin: 33; GRO alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; SDF-1 alpha: 13; RANTES: 42

Assay Range
[As determined for Lot 1:] Eotaxin: 0.61-2500 pg/mL; GRO alpha: 2.44-10000 pg/mL; IL-8: 2.44-10000 pg/mL; IP-10: 1.95-8000 pg/mL; MCP-1: 1.22-5000 pg/mL; MIP-1 alpha: 2.14-8750 pg/mL; MIP-1 beta: 6.10-25000 pg/mL; SDF-1 alpha: 17.09-70000 pg/mL; RANTES: 0.61-2500 pg/mL

Assay Sensitivity
[As determined for Lot 1:] Eotaxin: 1.4 pg/mL; GRO alpha: 2.8 pg/mL; IL-8: 1.2 pg/mL; IP-10: 0.3 pg/mL; MCP-1: 0.6 pg/mL; MIP-1 alpha: 1.1 pg/mL; MIP-1 beta: 4.7 pg/mL; SDF-1 alpha: 20.5 pg/mL; RANTES: 0.2 pg/mL

Cytokine & Chemokine 22-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Cytokine & Chemokine 22-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 22 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-1 alpha; G-CSF; IL-10; IL-17A; IL-1 beta; IL-6; TNF alpha; IL-4; GM-CSF; IFN gamma; IL-2; IL-5; IL-13; IL-12p70; Eotaxin; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-2; Rantes

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] IL-1 alpha: 12.2-50000 pg/mL; C-CSF (CSF-3): 3.7-15000 pg/mL; IL-10: 9.8-40000 pg/mL; IL-17A: 3.7-15000 pg/mL; IL-1 beta: 15.9-65000 pg/mL; IL-6: 4.9-20000 pg/mL; TNF alpha: 4.9-20000 pg/mL; IL-4: 1.2-5000 pg/mL; GM-CSF: 9.8-40000 pg/mL; IFN gamma: 9.8-40000 pg/mL; IL-2: 4.9-20000 pg/mL; IL-5: 2.4-10000 pg/mL; IL-13: 4.9-20000 pg/mL; IL-12p70: 9.8-40000 pg/mL; Eotaxin: 1.9-7700 pg/mL; GRO alpha: 11.4-46500 pg/mL; IP-10: 4.9-20100 pg/mL; MCP-1: 18.03-75000 pg/mL; MCP-3: 3.5-14200 pg/mL; MIP-1 alpha: 2.3-9500 pg/mL; MIP-2: 0.6-2400 pg/mL; Rantes: 85.5-350000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-1 alpha: 3.5 pg/mL; C-CSF (CSF-3): 0.3 pg/mL; IL-10: 1.6 pg/mL; IL-17A: 0.2 pg/mL; IL-1 beta: 1.1 pg/mL; IL-6: 1.9 pg/mL; TNF alpha: 0.4 pg/mL; IL-4: 0.1 pg/mL; GM-CSF: 1.3 pg/mL; IFN gamma: 0.6 pg/mL; IL-2: 1.9 pg/mL; IL-5: 0.3 pg/mL; IL-13: 0.3 pg/mL; IL-12p70: 5.9 pg/mL; Eotaxin: 1.1 pg/mL; GRO alpha: 1.4 pg/mL; IP-10: 0.9 pg/mL; MCP-1: 10.9 pg/mL; MCP-3: 1.1 pg/mL; MIP-1 alpha: 1.0 pg/mL; MIP-2: 0.1 pg/mL; Rantes: 6.1 pg/mL

6Ckine Recombinant Mouse Protein (Gibco™)

Recombinant 6Ckine is a bioactive protein intended for use in cell culture applications. Recombinant mouse 6Ckine is intended for use in cell culture applications. 6Ckine is a chemokine involved in chemotaxis of specific cell types.

Th1/Th2 Cytokine & Chemokine 20-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen™)

The Mouse Th1/Th2 Cytokine & Chemokine I 20-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 20 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; GRO-alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
IFN gamma: 38; IL-12p70: 39; IL-13: 35; IL-1 beta: 19; IL-2: 20; IL-4: 26; IL-5: 27; IL-6: 28; TNF alpha: 45; GM-CSF: 42; IL-18: 66; GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62

Assay Range
[As determined for Lot 1:] IFN gamma: 1.2-5000 pg/mL; IL-12p70: 2.4-10000 pg/mL; IL-13: 2.4-10000 pg/mL; IL-1 beta: 1.2-5000 pg/mL; IL-2: 1.2-5000 pg/mL; IL-4: 1.2-5000 pg/mL; IL-5: 2.4-10000 pg/mL; IL-6: 4.9-20000 pg/mL; TNF alpha: 3.7-15000 pg/mL; GM-CSF: 2.4-10000 pg/mL; IL-18: 36.6-150000 pg/mL; Gro alpha: 1.7-7000 pg/mL; IP-10: 0.5-2000 pg/mL; MCP-1: 7.3-30000 pg/mL; MCP-3: 0.2-1000 pg/mL; MIP-1 alpha: 0.5-2000 pg/mL; MIP-1 beta: 1.2-5000 pg/mL; MIP-2: 0.7-3000 pg/mL; RANTES: 2.4-10000 pg/mL; Eotaxin: 0.5-2000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.09 pg/mL; IL-12p70: 0.21 pg/mL; IL-13: 0.16 pg/mL; IL-1 beta: 0.14 pg/mL; IL-2: 0.10 pg/mL; IL-4: 0.03 pg/mL; IL-5: 0.32 pg/mL; IL-6: 0.21 pg/mL; TNF alpha: 0.39 pg/mL; GM-CSF: 0.19 pg/mL; IL-18: 9.95 pg/mL; Gro alpha: 0.05 pg/mL; IP-10: 0.26 pg/mL; MCP-1: 3.43 pg/mL; MCP-3: 0.15 pg/mL; MIP-1 alpha: 0.13 pg/mL; MIP-1 beta: 1.16 pg/mL; MIP-2: 0.37 pg/mL; RANTES: 0.35 pg/mL; Eotaxin: 0.01 pg/mL