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CD4 Monoclonal Antibody (RPA-T4), Super Bright 645, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (SK3 (SK-3)), eVolve 655, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

MagniSort™ Human CD4 T cell 2-Step Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes. It has been optimized for the isolation of CD4+ T cells from human peripheral blood monocuclear cells, utilizing biotinylated Anti-Human CD14 and Anti-Human CD4 antibodies, as well as streptavidin-coated magnetic beads. In the first step, monocytes are bound by Anti-Human CD14 antibody and then magnetic beads. When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step. In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads. Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted.

After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse CD4 (Invitrogen™)

Dynabeads® Mouse CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD4+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD4+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD4+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD4+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD4 beads are coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on mouse T helper cells. Dynabeads® Mouse CD4 binds to CD4+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD4+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD4 can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD4+ cells in about 30 minutes. Dynabeads® Mouse CD4 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use DETACHaBEAD® Mouse CD4 in combination with this products (for bead- and antibody-free cells) or Dynabeads® FlowComp Mouse CD4 Cells (complete kit for bead-free cells).

Select what is right for you from our range of Mouse CD4 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

CD4 Monoclonal Antibody (GK1.5), Biotin, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (S3.5), Pacific Blue (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 700, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (RPA-T4), FITC, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (GK1.5), PE-Cyanine5, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (RM4-5), Biotin, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (RM4-5), eFluor 615, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for IF, ICC, IHC (F)

CD4 Monoclonal Antibody (OKT4 (OKT-4)), APC-eFluor 780, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (4SM95), eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Western Blot, IHC (P)

CD4 Monoclonal Antibody (S3.5), Alexa Fluor 700 (Invitrogen™)

CD4 Monoclonal Antibody for Flow

CD4 Monoclonal Antibody (RM4-5), Super Bright 702, eBioscience™ (Invitrogen™)

CD4 Monoclonal Antibody for Flow