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eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine CD8 Activation 1 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

CD8+ T cells, or cytotoxic T lymphocytes, are immune cells that are responsible for the detection and clearance of virally or bacterically infected host cells. Upon recognition of antigen displayed on the surface of infected cells, activated CD8+ T cells upregulate markers of early activation such as CD69 and FAS ligand, followed by the release of granules containing granzymes and perforin as well as secretion of cytokines such as IFN gamma and TNF alpha. Granzymes and perforin act in concert to directly lyse infected target cells, while cytokines have paracrine effects including the recruitment and activation of macrophages, lymphocytes, and PMNs. IFN gamma is important for the upregulation of MHC class I and II on nearby cells as well as promoting Th1 cell differentiation.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

CD3/CD4/CD8 Monoclonal Antibody, FITC, PE, TRI-COLOR (Invitrogen™)

CD3/CD4/CD8 Cocktail Antibody for Flow

CD8 Monoclonal Antibody (3B5), Pacific Orange (Invitrogen™)

CD8 Monoclonal Antibody for Flow

CD8 alpha Monoclonal Antibody (3B5), Alexa Fluor 647 (Invitrogen™)

CD8 Monoclonal Antibody for Flow

Lab Vision™ CD8, Rabbit Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in immunohistochemistry applications with Thermo Scientific™ CD8, Rabbit Monoclonal Antibody.

CD8 Monoclonal Antibody (RPA-T8), Super Bright 645, eBioscience™ (Invitrogen™)

CD8a Monoclonal Antibody for Flow

CD8 Monoclonal Antibody (3B5), APC-Alexa Fluor 750 (Invitrogen™)

CD8 Monoclonal Antibody for Flow

CD8 alpha Monoclonal Antibody (5H10), FITC (Invitrogen™)

CD8 alpha Monoclonal Antibody for Flow

CD8 Monoclonal Antibody (3B5), Qdot 655 (Invitrogen™)

CD8 Monoclonal Antibody for Flow

CD8 Monoclonal Antibody (3B5), PE (Invitrogen™)

CD8 Monoclonal Antibody for Flow

CD8 beta Monoclonal Antibody (OX8), FITC (Invitrogen™)

CD8 alpha Monoclonal Antibody for Flow

MagniSort™ Mouse CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 T cell Enrichment Kit is designed for the magnetic separation of mouse CD8 T cells by negative selection. It has been optimized for the isolation of mouse CD8 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Mouse CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Memory T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Memory T cell Enrichment Kit is designed for the magnetic separation of CD8 memory T cells by negative selection. It has been optimized for the isolation of human CD8 memory T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 memory T cells untouched and free in solution.

The MagniSort™ Human CD8 Memory T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RA Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

CD8 alpha Monoclonal Antibody (5H10), Alexa Fluor 405 (Invitrogen™)

CD8 alpha Monoclonal Antibody for Flow

Lab Vision™ CD8 Ab-1, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in immunohistochemistry experiments with Thermo Scientific™ CD8 Ab-1, Mouse Monoclonal Antibody.