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CTLA4 Recombinant Mouse Protein, His Tag

Cytotoxic T-Lymphocyte-Associated 4 (CTLA4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 µg/ml (100 µl/well) can mouse CD80-Fc (cat:50446-M02H) with a linear range of 0.78-62.5 ng/ml.
2. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 µg/ml (100 µl/well) can human B7-1-Fc (Cat:10698-H03H) with a linear range of 0.78-125 ng/ml..

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

This protein is manufactured by Sino Biological Inc.

CTLA4 (CTLA-4) Recombinant Human Protein, hIgG1-Fc.His Tag

Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), recombinant human protein is supplied as a lyophilized powder.

This recombinant protein was expressed from a DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: This recombinant protein has not been tested.

Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by SINO Biological.

CTLA4 Monoclonal Antibody (OTI8F1), TrueMAB™ (OriGene)

CTLA4 Monoclonal Antibody for Western blot, Flow

CTLA4 Monoclonal Antibody (OTI1E2), TrueMAB™ (OriGene)

CTLA4 Monoclonal Antibody for Western blot, IF, ICC

CTLA4 Monoclonal Antibody (UMAB249), UltraMAB™ (OriGene)

CTLA4 Monoclonal Antibody for IHC (P)

CTLA4 Monoclonal Antibody (OTI1G10), TrueMAB™ (OriGene)

CTLA4 Monoclonal Antibody for IHC (P)

CTLA4 Monoclonal Antibody (OTI8D12), TrueMAB™ (OriGene)

CTLA4 Monoclonal Antibody for Western blot

CTLA4 Monoclonal Antibody (OTI3A5), TrueMAB™ (OriGene)

CTLA4 Monoclonal Antibody for Flow

CTLA-4 Mouse ELISA Kit (Invitrogen™)

CTLA-4 Mouse ELISA Kit for ELISA

CTLA-4 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CTLA-4 Simplex ProcartaPlex kit measures CTLA-4 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

CTLA-4 (Soluble) Human ELISA Kit (Invitrogen™)

CTLA-4 (Soluble) Human ELISA Kit for ELISA

CTLA-4 Polyclonal Antibody (Invitrogen™)

CTLA-4 Polyclonal Antibody for Western Blot, IF, ICC, Flow

CTLA-4 Polyclonal Antibody (Invitrogen™)

CTLA-4 Polyclonal Antibody for Western Blot, IHC (P)

Clathrin Light Chain A Polyclonal Antibody (Invitrogen™)

Clathrin Light Chain A Polyclonal Antibody for Western Blot

CTLA-4 Monoclonal Antibody (WKH 203), Biotin (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203) (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203), Biotin (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203), FITC (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203), FITC (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203), PE (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH203), PE (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (A3.4H2.H12), FITC (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (A3.4H2.H12) (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (WKH 203), PE (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (AS32) (Invitrogen™)

CTLA-4 Monoclonal Antibody for Neu

CTLA-4 Monoclonal Antibody (1B8), PE (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow

CTLA-4 Monoclonal Antibody (1B8), FITC (Invitrogen™)

CTLA-4 Monoclonal Antibody for Flow