Manual / Product Insert

Fluo-4 Direct Calcium Assay Kits

Version: 04-03-2009

Citations & References

Detection in Vivo of Very Rapid Red Light-Induced Calcium-Sensitive Protein Phosphorylation in Etiolated Wheat (Triticum aestivum) Leaf Protoplasts.

  • Authors: Fallon KM, Shacklock PS, Trewavas AJ
  • Journal: Plant Physiol (1993) 101:1039-1045
  • PubMed ID: 12231756
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Citations & References

Mechanisms of calcium influx into hippocampal spines: heterogeneity among spines, coincidence detection by NMDA receptors, and optical quantal analysis.

  • Authors: Yuste R, Majewska A, Cash SS, Denk W
  • Journal: J Neurosci (1999) 19:1976-1987
  • PubMed ID: 10066251

Citations & References

Simultaneous detection of intracellular free calcium and zinc using fura-2FF and FluoZin-3.

  • Authors: Devinney MJ, Reynolds IJ, Dineley KE
  • Journal: Cell Calcium (2005) 37:225-232
  • PubMed ID: 15670869
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Citations & References

Genome-wide analysis of genes related to kidney stone formation and elimination in the calcium oxalate nephrolithiasis model mouse: detection of stone-preventive factors and involvement of macrophage activity.

  • Authors: Okada A, Yasui T, Hamamoto S, Hirose M, Kubota Y, Itoh Y, Tozawa K, Hayashi Y, Kohri K
  • Journal: J Bone Miner Res 2009; (24):5 908-924
  • PubMed ID: 19113933

Citations & References

Calcium release domains in mammalian skeletal muscle studied with two-photon imaging and spot detection techniques.

  • Authors: Gómez J, Neco P, DiFranco M, Vergara JL
  • Journal: J Gen Physiol (2006) 127:623-637
  • PubMed ID: 16735751

Citations & References

Confocal near-membrane detection of calcium in cardiac myocytes.

  • Authors: Blatter LA, Niggli E
  • Journal: Cell Calcium (1998) 23:269-279
  • PubMed ID: 9681190

Citations & References

A simple numerical model of calcium spark formation and detection in cardiac myocytes.

  • Authors: Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H
  • Journal: Biophys J (1998) 75:15-32
  • PubMed ID: 9649364
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Citations & References

Calcium ion fluorescence detection using liposomes containing Alexa-labeled calmodulin.

  • Authors: Nguyen T, Rosenzweig Z
  • Journal: Anal Bioanal Chem (2002) 374:69-74
  • PubMed ID: 12207244
Catalog #
  • C13844(Discontinued)

Product FAQ

Can I fix my cells after loading with Fluo-4 AM dye for the detection of calcium?


No. Since Fluo-4 AM isn’t covalently bound to any cellular components and fixation compromises the membrane, the dye would not be retained by the cell.

Answer Id: E6494

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Citations & References

Detection of Ca(2+)-transients elicited by flash photolysis of DM-nitrophen with a fast calcium indicator.

  • Authors: Escobar AL, Cifuentes F, Vergara JL
  • Journal: FEBS Lett (1995) 364:335-338
  • PubMed ID: 7758592

Citations & References

Presynaptic calcium signals during neurotransmitter release: detection with fluorescent indicators and other calcium chelators.

  • Authors: Augustine GJ, Adler EM, Charlton MP, Hans M, Swandulla D, Zipser K
  • Journal: J Physiol Paris (1992) 86:129-134
  • PubMed ID: 1364192
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Citations & References

Localized detection of action potential-induced presynaptic calcium transients at a Xenopus neuromuscular junction.

  • Authors: DiGregorio DA, Vergara JL
  • Journal: J Physiol (1997) 505 ( Pt 3):585-592
  • PubMed ID: 9457637
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Product Literature

Application Note: Separation of Calcium, Magnesium and Counterions in a Dietary Supplement Using Multi-mode Liquid Chromatography with Charged Aerosol Detection

Citations & References

Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen.

  • Authors: Szász F, Sirivaidyapong S, Cheng FP, Voorhout WF, Marks A, Colenbrander B, Solti And L, Gadella BM
  • Journal: Mol Reprod Dev (2000) 55:289-298
  • PubMed ID: 10657048
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