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User Guide: 3130/3130xL Genetic Analyzers-3130 Series Data Collection Software 4 Getting Started Manual / Product Insert

  • Version: 4477796 Rev.A (05.2012)
Catalog #

Application Notes & Tutorials: Applied Biosystems 3130 and 3130xl Genetic Analyzers: One Polymer, One Capillary Array Length, Maximum Performance Product Literature

Development of a flow injection capillary chemiluminescent ELISA using an imprinted polymer instead of the antibody. Citations & References

  • Authors: Surugiu L, Svitel J, Ye L, Haupt K, Danielsson B
  • Journal: Anal Chem (2001) 73:4388-4392
  • PubMed ID: 11569836
Catalog # L8455(Discontinued)

Functionalized gold nanoparticles as additive to form polymer/metal composite matrix for improved DNA sequencing by capillary electrophoresis Citations & References

  • Authors: Zhou, D; Yang, LP; Yang, RM; Song, WH; Peng, SH; Wang, YM
  • Journal: Talanta 2009 1:195-201

Evaluation of capillary electrophoresis in polymer solutions with laser-induced fluorescence detection for the automated detection of T-cell gene rearrangements in lymphoproliferative disorders. Citations & References

  • Authors: Oda RP, Wick MJ, Rueckert LM, Lust JA, Landers JP
  • Journal: Electrophoresis (1996) 17:1491-1498
  • PubMed ID: 8905266
Catalog #

Effective capillary electrophoresis-based heteroduplex analysis through optimization of surface coating and polymer networks. Citations & References

  • Authors: Tian H, Brody LC, Mao D, Landers JP
  • Journal: Anal Chem (2000) 72:5483-5492
  • PubMed ID: 11080904
Catalog #
  • P11495
  • P11496
  • P7581
  • P7589
  • Y3603
  • E1305(Discontinued)
  • E3565(Discontinued)
  • R21495(Discontinued)
  • R32701(Discontinued)
  • R32716(Discontinued)
  • D7520
  • R21496

DNA typing of a polymerase chain reaction amplified D1S80/amelogenin multiplex using capillary electrophoresis and a mixed entangled polymer matrix. Citations & References

  • Authors: Isenberg AR, McCord BR, Koons BW, Budowle B, Allen RO
  • Journal: Electrophoresis (1996) 17:1505-1511
  • PubMed ID: 8905268
Catalog # Y3603

Do we need dedicated capillary arrays for each polymer on the 3130 system? Product FAQ

Answer

Dedicated capillary arrays for each polymer are not necessary. But if you need to switch polymer types, for example from POP-6 polymer to POP-7 polymer, you would need to use the Change Polymer Type Wizard to ensure good data quality after switching polymer types. A different spectral calibration for each dye set is required for each polymer type.

Answer Id:: E2375

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Characterization of the Oligomeric Dispersion of Poly(oxyalkylene)diamine Polymers by Precolumn Derivatization and Capillary Zone Electrophoresis With Fluorescence Detection. Citations & References

  • Authors: Amankwa LN, Scholl J, Kuhr WG
  • Journal: Anal Chem (1990) 62:2189-2189
  • PubMed ID: 0
Catalog # N1138

I have an Applied Biosystems 3130/3130xl Genetic Analyzer. When do my consumables expire? Product FAQ

Answer

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id:: E12705

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What is the white residue around the array knob on my Applied Biosystems 3730/3730xl DNA Analyzer? Product FAQ

Answer

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Answer Id:: E12753

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With my Applied Biosystems 3130/3130xl Genetic Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it? Product FAQ

Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id:: E12716

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What is the white residue around the array knob on my Applied Biosystems 3130/3130xl Genetic Analyzer? Product FAQ

Answer

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Answer Id:: E12718

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My Applied Biosystems 3500/3500xL Genetic Analyzer consumables have expired, but how long past expiration can I really use them? Product FAQ

Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id:: E12769

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