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Cell Analysis Software and Informatics Products Product Literature

Is EquiPhi29 DNA Polymerase suitable for single cell DNA amplification? Product FAQ

Answer

Yes. The recommended protocol is supplied in the manual (https://www.thermofisher.com/order/catalog/product/A39390). Alternatively, amplification from single cells using EquiPhi29 DNA polymerase can be reviewed in the literature:

Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles.
Authors: Stepanauskas R, Fergusson EA, Brown J, Poulton NJ, Tupper B, Labonté JM, Becraft ED, Brown JM, Pachiadaki MG, Povilaitis T, Thompson BP, Mascena CJ, Bellows WK, Lubys A
Journal: Nat Commun 2017; 8(1):84
PubMed ID: 28729688

Answer Id:: E16600

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Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. Citations & References

  • Authors: Love KR, Politano TJ, Panagiotou V, Jiang B, Stadheim TA, Love JC,
  • Journal: PLoS One
  • PubMed ID: 22685548
Catalog # G10362

Mitochondrial membrane permeabilization and superoxide production during apoptosis. A single-cell analysis. Citations & References

  • Authors: Düssmann H, Kögel D, Rehm M, Prehn JH
  • Journal: J Biol Chem
  • PubMed ID: 12560329
Catalog #

Cellular metabolism and oxidative stress as a possible determinant for longevity in small breed and large breed dogs. Citations & References

  • Authors: Jimenez AG, Winward J, Beattie U, Cipolli W
  • Journal: PLoS One
  • PubMed ID: 29694441
Catalog # C10445

Inter-relationship between mitochondrial function and susceptibility to oxidative stress in red- and white-blooded Antarctic notothenioid fishes. Citations & References

  • Authors: Mueller IA, Grim JM, Beers JM, Crockett EL, O'Brien KM,
  • Journal: J Exp Biol
  • PubMed ID: 22031737
Catalog # C10445

Image-iT Lipid Peroxidation Kit Manual / Product Insert

  • Version: 6 May 2012
Catalog # C10445

Role of membrane cholesterol in spontaneous exocytosis at frog neuromuscular synapses: reactive oxygen species-calcium interplay. Citations & References

  • Authors: Petrov AM, Yakovleva AA, Zefirov AL
  • Journal: J Physiol
  • PubMed ID: 25326454
Catalog # C10445

The human chromosome content in human x rodent somatic cell hybrids analyzed by a screening technique using Alu PCR. Citations & References

  • Authors: Bicknell DC; Markie D; Spurr NK; Bodmer WF
  • Journal: Genomics
Catalog #

Measurement of transient cDNA expression in mammalian cells using flow cytometric cell analysis and sorting. Citations & References

  • Authors: Rice GC, Pennica D, Borree JA, Williams SR
  • Journal: Cytometry
  • PubMed ID: 2036916

Single-cell transcript analysis of pancreas development. Citations & References

  • Authors: Chiang MK, Melton DA
  • Journal: Dev Cell
  • PubMed ID: 12636919
Catalog #

Wastewater Analysis Products Product Literature

How are the Neon Kit cell viability and transfection efficiency determined by your R&D team? Product FAQ

Answer

Cells were analyzed for viability and transfection efficiencies 24 hours or 48 hours post-electroporation using a Guava PCA-96 Cell Analysis System. LIVE/DEAD cell viability assay populations were calculated by propidium iodide staining (1:2000). The percent of transfected cells was calculated by dividing the number of GFP positive cells by the total population and recorded as transfection efficiencies. The percent of dead cells was calculated by dividing the number of PI-stained cells by the total population

Answer Id:: E5491

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What eBioscience antibodies can I use to label a particular cell type for flow cytometric analysis? Product FAQ

Answer

Use our Mouse (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/mouse-cd-other-cellular-antigen.html) and Human (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/human-cd-other-cellular-antigens.html) CD charts to help identify the expression pattern of the different antigens of interest.

Answer Id:: E14449

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