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Cell Analysis Software and Informatics Products Product Literature

Is EquiPhi29 DNA Polymerase suitable for single cell DNA amplification? Product FAQ

Answer

Yes. The recommended protocol is supplied in the manual (https://www.thermofisher.com/order/catalog/product/A39390). Alternatively, amplification from single cells using EquiPhi29 DNA polymerase can be reviewed in the literature:

Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles.
Authors: Stepanauskas R, Fergusson EA, Brown J, Poulton NJ, Tupper B, Labonté JM, Becraft ED, Brown JM, Pachiadaki MG, Povilaitis T, Thompson BP, Mascena CJ, Bellows WK, Lubys A
Journal: Nat Commun 2017; 8(1):84
PubMed ID: 28729688

Answer Id:: E16600

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Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. Citations & References

  • Authors: Love KR, Politano TJ, Panagiotou V, Jiang B, Stadheim TA, Love JC,
  • Journal: PLoS One
  • PubMed ID: 22685548

Mitochondrial membrane permeabilization and superoxide production during apoptosis. A single-cell analysis. Citations & References

  • Authors: Düssmann H, Kögel D, Rehm M, Prehn JH
  • Journal: J Biol Chem
  • PubMed ID: 12560329
Catalog #

The human chromosome content in human x rodent somatic cell hybrids analyzed by a screening technique using Alu PCR. Citations & References

  • Authors: Bicknell DC; Markie D; Spurr NK; Bodmer WF
  • Journal: Genomics
Catalog #

Measurement of transient cDNA expression in mammalian cells using flow cytometric cell analysis and sorting. Citations & References

  • Authors: Rice GC, Pennica D, Borree JA, Williams SR
  • Journal: Cytometry
  • PubMed ID: 2036916

Single-cell transcript analysis of pancreas development. Citations & References

  • Authors: Chiang MK, Melton DA
  • Journal: Dev Cell
  • PubMed ID: 12636919

Image-iT Lipid Peroxidation Kit Manual / Product Insert

  • Version: 6 May 2012
Catalog # C10445

What eBioscience antibodies can I use to label a particular cell type for flow cytometric analysis? Product FAQ

Answer

Use our Mouse (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/mouse-cd-other-cellular-antigen.html) and Human (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/human-cd-other-cellular-antigens.html) CD charts to help identify the expression pattern of the different antigens of interest.

Answer Id:: E14449

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How are the Neon Kit cell viability and transfection efficiency determined by your R&D team? Product FAQ

Answer

Cells were analyzed for viability and transfection efficiencies 24 hours or 48 hours post-electroporation using a Guava PCA-96 Cell Analysis System. LIVE/DEAD cell viability assay populations were calculated by propidium iodide staining (1:2000). The percent of transfected cells was calculated by dividing the number of GFP positive cells by the total population and recorded as transfection efficiencies. The percent of dead cells was calculated by dividing the number of PI-stained cells by the total population

Answer Id:: E5491

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Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Citations & References

  • Authors: Nolan GP, Fiering S, Nicolas JF, Herzenberg LA
  • Journal: Proc Natl Acad Sci U S A
  • PubMed ID: 3128790
Catalog #

How can I use an Antaris FT-NIR analyzer for in-line process analysis? Product FAQ

Answer

If the process environment has water hose down, CIP, dust, high temperature, corrosive or explosive chemicals, the Antaris FT-NIR analyzer needs to be placed in a safe area or enclosed in an environmentally stabilized enclosure. Fiber optics run from the NIR analyzer to probes or flow cells installed in production process pipes, tanks, hoppers, conveyors, reactors, etc. The fiber optics carry the NIR source light to the probe sampling window and then carry the light after it has interacted with the sample back to the NIR analyzer detector. The end of the probe will have a window or an air gap for reflection or transmission analysis. The product being analyzed must be self-cleaning or the probe engineered to automatically clean itself by high pressure air. The computer that controls the NIR analyzer is also located in the safe area with Thermo Scientific RESULT Software exporting NIR results to text or Microsoft Excel files, LIMS, OPC or by 4-20 mA.

Answer Id:: E20699

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What information do you have regarding the cross-reactivity of your eBioscience antibodies? Product FAQ

Answer

Information about known cross-reactivity of an antibody is given on the Technical Data Sheets. We have done some in-house testing to analyze cross-reactivity of anti-human antibodies amongst non-human primates. The results of these tests are summarized in our Cross-Reactivity Charts (www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources).

Answer Id:: E14443

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Do I have to stain with surface staining antibodies before starting the PrimeFlow RNA Assay? Product FAQ

Answer

Staining for some surface markers may be done after fixation and permeabilization. Please see the Antibody Clone Performance Following Fixation/Permeabilization table on the website (www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/antibody-fixation-considerations.html) and refer to the column for “After IC Fixation and Perm Wash” to determine if the antibody clone will recognize a fixed epitope.

Answer Id:: E14576

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What concentration of my antibody should I use for cell analysis? Product FAQ

Answer

An optimal concentration may be between 1-10 µg/mL for cell and tissue labeling for microscopy, or 0.2-5 µg/mL for flow cytometry. A range of concentrations should be tested to determine what is optimal.

Answer Id:: E14774

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