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Cell Culture Media - MarrowMAX Product Literature

Cell Culture Media and Reagent Product Literature

Exosome CD63 (protocol for flow cytometry) Manual / Product Insert

  • Pub. No.: 2ec9d76e785774d7b12b3ca8925cd91264b50e7a
  • Version: Rev 1.0 (2 June 2013)
Catalog # 10606D

Exosome CD63 (protocol for western blot) Manual / Product Insert

  • Pub. No.: 4696aed8b3bddf96d89883e506299510d5010fd5
  • Version: Rev 1.0 (2 June 2013)
Catalog # 10606D

Are Nalgene Square PETG Media Bottles with Closure suitable for making aliquots of cell culture media? Product FAQ

Answer

Nalgene Square PETG Media Bottles with Closure can be used for cell culture media as they are sterile and non-pyrogenic.
Note: Make sure everything is done aseptically.

Answer Id: E19731

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BenchStable Cell Culture Media - Green Fact Sheet Product Literature

What is the best way to get a high yield of miRNA from exosomes isolated from cell culture media? Product FAQ

Answer

A pure sample of exosomes contains very low amounts of RNA. Therefore, the best way to increase yeild is to scale up the isolation. For cell culture media, you can try starting with 100 mL of sample.

Answer Id: E7420

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What are the main differences between insect cell culture and mammalian cell culture? Product FAQ

Answer

Insect cells are much more fragile than a lot of mammalian cell lines. They suffer much more damage than mammalian cells from overgrowth and over-splitting. Never let cells go above 8 x 10E6 cells/mL or grow at densities less than 0.5 x 10E6 cells/mL in suspension. Insect cells require a little more osmotic pressure than mammalian cells (340 µOsM). Insect cells use a lot of O2, especially during protein expression. Insect cell culture media is more acidic than mammalian media (pH 6.0-6.4). The insect cell culture media is phosphate buffer based. Therefore, no CO2 is needed to maintain the pH.

Answer Id: E11937

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Do you measure the refractive index and viscosity for your cell culture media products? Product FAQ

Answer

We do not measure the refractive index or viscosity for our cell culture media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E17424

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Total Exosome Isolation (from cell culture media) Manual / Product Insert

  • Pub. No.: ab902263560e96e43150ca68f668ab3d507dc4ce
  • Version: 1.0
Catalog # 4478359

Article: Next Generation Cell Culture Media and Feeds System Product Literature

I am getting poor recovery with my ProcartaPlex multiplex immunoassay. What can I do? Product FAQ

Answer

Here are possible causes and solutions:
- The wrong cell culture media may have been used to prepare the standards. We recommend using the same cell culture media that was used to culture the cells.
- The samples and antigen standards may not have been stored on ice. We recommend preparing the samples and standards on ice before setting up the assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E19267

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Article: Benefits of customizable cell culture media in therapeutic development Product Literature

What is currently the typical method for exosome isolation? Product FAQ

Answer

Traditional isolation of exosomes from cell culture media and body fluids is a tedious and difficult process with the most widely used approach based on ultracentrifugation in combination with sucrose density gradients or sucrose cushions to float the relatively low-density exosomes away from other vesicles and particles. These protocols can range in time from 8 to 30 hrs and require an ultracentrifuge and extensive training to ensure successful isolation of exosomes. Despite these drawbacks, ultracentrifugation is still the most popular approach for exosome isolation. However, within the last 2 years, several exosome isolation reagents for cell culture media and various body fluids have become commercially available. Thermo Fisher Scientific Total Exosome Isolation reagents allow recovery of exosomes using a very short and reliable protocol which is becoming more and more popular.

Answer Id: E7449

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What aspects of cell culture media can be customized? Product FAQ

Answer

The Gibco Media Configurator allows you to add, remove, and adjust the concentration of components. In addition, you can select from a range of packaging, QC tests, and either cGMP or non-cGMP manufacturing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11845

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