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What is the difference between your Nunc Biobanking and Cell Culture Cryogenic Tubes with internal threads and external threads? Product FAQ

Answer

Internally threaded Nunc Biobanking and Cell Culture Tubes are designed for vapor phase liquid nitrogen freezing. The cap incorporates a silicon gasket to enhance sealing properties.
Externally threaded Nunc Biobanking and Cell Culture Tubes are designed for mechanical freezing. The external screw cap reduces risk of contamination due to handling.

Answer Id:: E17755

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Can I autoclave Nunc Biobanking and Cell Culture Cryogenic Tubes? Product FAQ

Answer

We do not recommend autoclaving Nunc Biobanking and Cell Culture Tubes, even though they are made of polypropylene with polyethylene caps and stoppers.

Answer Id:: E17754

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Can I immerse Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid nitrogen (liquid phase) for storage? Product FAQ

Answer

Of the Nunc Biobanking and Cell Culture Cryogenic Tubes, we recommend the internally threaded design, incorporating a silicon gasket, for liquid nitrogen immersion. At ultra low temperatures, any sealing material becomes inflexible. A theoretical risk of leakage is present when any mechanical closure is used. To facilitate leakproof storage, mechanically closed containers must be hermetically sealed in a protective envelope.

The Nunc cryo products includes Nunc CryoFlex Tube Wrap, a heat sealable polyethylene tubing designed for cryogenic tube encasement. The use of cryogenic tubes for freezing in liquid nitrogen (liquid phase) storage without CryoFlex tubing poses a safety hazard.
WARNING: Do not use Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid phase of liquid nitrogen unless correctly sealed in Nunc CryoFlex Tube Wrap (Cat. No. 343958). Improper use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up, resulting in possible explosion or biohazard release. Use appropriate safety procedures as outlined in the Nalge Nunc International Cryopreservation Manual when handling and disposing of the vials.

Answer Id:: E17756

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Will the heat required to properly seal Nunc CryoFlex Tube Wrap adversely affect my cells? Product FAQ

Answer

Nunc Biobanking and Cell Culture Cryogenic Tubes are normally placed in a 4 degrees C ice bath during cell inoculation in the cryogenic tube. The heat required to shrink the polyethylene Nunc CryoFlex Tube Wrap will not raise the internal temperature enough to damage the cells. At this stage, cryoprotective agents are added.

Answer Id:: E17758

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Are your Nunc CryoColor Vial Closure Coders removable? Product FAQ

Answer

Once inserted, the Nunc CryoColor Vial Closure Coders cannot be removed.

Answer Id:: E17759

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What protocol should I follow for thawing cells, if my Nunc Biobanking and Cell Culture Cryogenic Tubes were previously immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap? Product FAQ

Answer

We recommend the following protocol for thawing cells immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap:
- Wear appropriate protective clothing including gloves, face guard, etc., during all transfer processes.
- The Nunc Biobanking and Cell Culture Cryogenic Tubes should be stored at least 24 hr in the gas phase of the liquid nitrogen freezer. This should allow trapped liquid nitrogen to evaporate and escape slowly, thereby reducing the possible pressure in the cryogenic tube.
- After at least 24 hr storage in the gas phase, the cryogenic tube may be put into a water bath and covered with a towel.
- After thawing, the contents of the cryogenic tube can be transferred.

Answer Id:: E17757

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Technical Bulletin: Cryopreservation of Mammalian Cells - Protocols Product Literature

细胞培养的技巧及相关产品选择 Product Literature

Should I choose externally or internally threaded cryostorage vials? Product FAQ

Answer

Both types are designed and tested for secure cryogenic storage, so it’s largely a matter of personal preference. In an externally threaded vial (in which the cap covers the entire top portion of the vial itself), the underside of the cap is protected when unscrewed), which can minimize the potential for cross-contamination. In an internally threaded vial (in which the vial threads are inside the neck area and the cap inserts like a plug), the cap is the same diameter as the vial itself which can be helpful in certain types of dense storage applications.

Answer Id:: E15920

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Can I use your Nunc cryotubes or Nalgene cryotubes in liquid nitrogen? Product FAQ

Answer

Liquid nitrogen has very low surface tension, so it can flow into any of our cryostorage vials even if they are tightly capped. This creates significant potential for cross-contamination in storage as well as risk of injury from explosion when the vials are removed from storage and the liquid nitrogen boils off. The only option to mitigate this is Nunc Cryoflex tubing (e.g., Cat. No. 343958), which results in a heat-sealed barrier layer around each vial. Alternatively, storage in the vapor phase of liquid nitrogen is acceptable unless otherwise noted.

Answer Id:: E15919

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Sample Tracking Product Literature

Brochure: Streamline your Cryogenic Storage [EN] Product Literature

Committed to perfecting cell culture at every scale: Thermo Scientific Nunc Cell Factory Systems Product Literature

Product Brochure: Nunc Solutions for Cell Culture and Growth Product Literature

Nunc细胞培养和冻存产品 Product Literature

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