Documents & Support

1 - 15 of 17059 results

293A Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R70507

What kind of Flp-In T-REx cell lines do you offer? Product FAQ

Answer

We offer the Flp-In T-REx system that contains pFRT/lacZeo and pcDNA6/TR stably integrated into HEK 293 cells. This cell line has been functionally tested for its ability to regulate expression.

Answer Id:: E9165

Was this answer helpful?

Yes No

Thank you for your response

User Guide: Growth and Maintenance of Flp-In Cell Lines Manual / Product Insert

  • Version: 4.0
Catalog #

Flp-In™-Jurkat Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R76207

Flp-In™-3T3 Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R76107

Flp-In™-293 Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R75007

Flp-In™-CHO Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R75807

293FT Cell Line Limited Use Label Licenses

Limited Use Label Licenses
Catalog # R70007

What are the different kinds of T-REx cell lines you offer? Product FAQ

Answer

We offer T-REx-293, -HeLa, -CHO, and -Jurkat cell lines. These cell lines are derived by transfection of parental cells with pcDNA6/TR followed by stable selection with blasticidin. They constitutively and stably express the TetR gene, allowing significant time and effort saving when using the T-REx system. These cell lines are functionally tested for expression by transient transfection with the positive control vector, pcDNA4/TO/lacZ. They exhibit extremely low basal expression levels of bGal in the repressed state and high expression upon induction with tetracycline.

Answer Id:: E9160

Was this answer helpful?

Yes No

Thank you for your response

I have cloned my gene of interest into pLenti6.3/TO/V5-DEST and would like to use one of your T-REx cell lines as the host for the lentiviral construct. Will that work? Product FAQ

Answer

Yes, you can use one of our T-REx cell lines as the host for your pLenti6.3/TO/V5-DEST lentiviral construct. However, please note that while the pLenti6.3/TO/V5-DEST vector is a HiPerform lentiviral vector containing the genetic elements WPRE and cPPT for enhancing viral titer and expression; the T-REx cell lines we offer do not contain these elements. Further, you can use these T-REx cell lines only for transient expression, because the Lenti6.3/TO/V5-DEST lentiviral expression construct, and the Tet repressor plasmid (pcDNA6/TR) that is stably integrated within the T-REx cells, both contain the blasticidin selection marker, making stable cell line development not possible.

Answer Id:: E9306

Was this answer helpful?

Yes No

Thank you for your response

User Guide: Growth and Maintenance of the 293A Cell Line Manual / Product Insert

  • Version: MAN0000303 Rev A.0 (06Jul2016)
Catalog # R70507

I am interested in using your conditional T-REx expression system to knock down my gene of interest. Do you offer any T-REx cell lines that stably express the Tet repressor? Product FAQ

Answer

Yes, we offer 4 T-REx cell lines: 293, HeLa, CHO, or Jurkat cells. Alternatively, you can create your own T-REx cell line that stably expresses the Tet repressor form the pcDNA6/TR.

Answer Id:: E10012

Was this answer helpful?

Yes No

Thank you for your response

CellSensor® Gli-bla NIH3T3 Cell Line Validation Packet Manual / Product Insert

  • Version: version 1.1
Catalog # K1642B

CellSensor® MMTV-bla HeLa Cell Line Validation Packet Manual / Product Insert

  • Version: version 1.1
Catalog # K1641B

User Guide: Growth and Maintenance of T-REx Cell Lines Manual / Product Insert

  • Version: 5.0
Catalog #
Results per page
spinner