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Annexin 24 from Capsicum annuum. X-ray structure and biochemical characterization. Citations & References

  • Authors: Hofmann A, Proust J, Dorowski A, Schantz R, Huber R
  • Journal: J Biol Chem
  • PubMed ID: 10713128
Catalog #
  • N6547
  • F1200
  • C1360
  • B6492(Discontinued)
  • F6799(Discontinued)
  • C194(Discontinued)
  • C1904(Discontinued)
  • C1359(Discontinued)
  • B8405

CCCTC-Binding Factor and the Transcription Factor T-bet Orchestrate T Helper 1 Cell-Specific Structure and Function at the Interferon-γ Locus Citations & References

  • Authors: Sekimata, M; Perez-Melgosa, M; Miller, SA; Weinmann, AS; Sabo, PJ; Sandstrom, R; Dorschner, MO; Stamatoyannopoulos, JA; Wilson, CB
  • Journal: IMMUNITY
Catalog #

Where can I go to get help with choosing dyes for labeling organelles and cell structures? Product FAQ

Answer

Visiting the imaging cell structure page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure.html) will help you get started choosing the best dyes and reagents for organelle labeling. Our Cell Staining Simulation tool (https://www.thermofisher.com/us/en/home/life-science/lab-data-management-analysis-software/lab-apps/cell-staining-tool.html) is also helpful, where you can see an example simulated cell image as you pick and choose reagents.

Answer Id:: E14776

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Are the cell liner trays reusable for the BenchPro 2100 Plasmid Processing Station? Product FAQ

Answer

No. The cell liner tray and lid are not reusable. They should be disposed of after each procedure. Each BenchPro 2100 reagent kit comes with a supply of cell liner trays and lids. Cell liner trays are not currently available for standalone purchase.

Answer Id:: E7768

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Embryonic gene expression resolved at the cellular level by fluorescence in situ hybridization. Citations & References

  • Authors: Paratore C, Suter U, Sommer L
  • Journal: Histochem Cell Biol
  • PubMed ID: 10429965
Catalog #
  • N6547
  • N6495
  • B6492(Discontinued)
  • T24831
  • T6500
  • B8405

What is the difference between XTT (Cat No. X6493) and CyQUANT XTT Cell Viability Assay (Cat No. X12223)? Product FAQ

Answer

XTT (Cat No. X6493) is 100 mg of XTT in solid form; you must resolubilize this into an aqueous solution or DMSO prior to use.

CyQUANT XTT Cell Viability Assay (Cat No. X12223) is a kit that provides two components: 10 vials each of the XTT Reagent and Electron Coupling Reagent. Both kit components are in solution in a ready-to-use format; mix 1 vial of the XTT Reagent and 1 vial of the Electron Coupling Reagent immediately before use.

Answer Id:: E19047

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Which is the most suitable sample fixation method? Product FAQ

Answer

The selection of the fixation method depends upon the sample and the desired outcome:
- Which cell/tissue structures need to be preserved?
- Is the fixation method compatible with the label or stain to be used?
For example, methods commonly used to fix samples for flow cytometry are totally unsuitable for electron microscopy.
Acetone/methanol or other alcohol fixatives disrupt lipid droplets/membranes and condense proteins, nucleic acids, and other cellular components. These reagents do not cross-link cellular components and thus limit masking of epitopes.
Cross-linking fixatives, such as formaldehyde, glutaraldehyde and bis-suberate, applied appropriately, are the best in retaining cell structure, but the cross-linking can mask epitopes and the samples may require further processing for antigen retrieval.
Using too low a concentration of fixative or too short an incubation time may result in a damaged sample; live cells may shrink, bleb, or rupture in response to the addition to the toxic fixative. Many enzymes survive fixation but are cross-linked in place to prevent their movement throughout the cell and outside the cell; if not cross-linked, they are free to migrate and disrupt various cellular components, resulting in a degraded cell/tissue structure.

Answer Id:: E18327

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Pyrimidinoceptor-mediated potentiation of inducible nitric-oxide synthase induction in J774 macrophages. Role of intracellular calcium. Citations & References

  • Authors: Chen BC, Chou CF, Lin WW
  • Journal: J Biol Chem
  • PubMed ID: 9792689
Catalog #

What samples are in the CytoScan HD and 750K reference model files? Product FAQ

Answer

The CytoScan HD and 750K reference model files are comprised of the same set of samples and include 380 microarrays that were run as part of a larger set of microarrays by nine operators processing ~48 unique samples in two rounds each, with randomization of the placement of DNA samples across the PCR plates and randomization of the reagents and instruments used. The source DNA includes

284 HapMap samples, including at least one replicate of each of 270 HapMap samples: 90 from each of the Yoruban, Asian, and Caucasian ethnic groups from cell line-derived DNA from the Coriell Institute of Medical Research

96 DNA samples from blood of phenotypically healthy male and female individuals obtained from BioServe Biotechnologies

The samples in this set were chosen to have been run by different operators and with different kits and reagents while still covering all the HapMap cell line ethnic groups, plus the normal blood samples of both sexes.

Answer Id:: E13847

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Expression of serotonin receptors in bone. Citations & References

  • Authors: Westbroek I, van der Plas A, de Rooij KE, Klein-Nulend J, Nijweide PJ
  • Journal: J Biol Chem
  • PubMed ID: 11387323
Catalog #

Three subunit a isoforms of mouse vacuolar H(+)-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. Citations & References

  • Authors: Toyomura T, Oka T, Yamaguchi C, Wada Y, Futai M
  • Journal: J Biol Chem
  • PubMed ID: 10722719
Catalog #

In vitro and in vivo anti-inflammatory activity of 17-O-acetylacuminolide through the inhibition of cytokines, NF-?B translocation and IKKß activity. Citations & References

  • Authors: Achoui M, Appleton D, Abdulla MA, Awang K, Mohd MA, Mustafa MR,
  • Journal: PLoS One
  • PubMed ID: 21152019
Catalog #
  • N01X1SHTLAS
  • G7921
  • ASN0000S1(Discontinued)
  • ASN00002P(Discontinued)
  • ASN00003L(Discontinued)

Is the protocol same for Exon 1.0 St and Gene 1.0 St arrays? Product FAQ

Answer

The protocols for the Gene 1.0 ST and Exon 1.0 ST arrays are the same until the fragmentation and labeling steps.
Depending on the input amount, please refer to the GeneChip WT Pico Reagent Kit or the GeneChip WT PLUS Reagent Kit for the protocol:
- WT Pico: 50 - 500ng of total RNA
- WT PLUS: ≥ 100pg of total RNA (approx. 10 cells)
From the hybridization step onwards, the differences in protocol are due to the format of the arrays. The Exon array is a 49 format array, whereas the Gene 1.0 ST array is a 169 format array.
For the fluidics step the following scripts should be used depending upon which array you are using:
- Gene 1.0 St array: FS450_0001
- Exon 1.0 St array: FS450_0007

Answer Id:: E18123

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Changes in E. coli cell envelope structure caused by uncouplers of active transport and colicin E1. Citations & References

  • Authors: Helgerson SL, Cramer WA
  • Journal: J Supramol Struct
  • PubMed ID: 828690
Catalog #

HtrA heat shock protease interacts with phospholipid membranes and undergoes conformational changes. Citations & References

  • Authors: Skórko-Glonek J, Lipinska B, Krzewski K, Zolese G, Bertoli E, Tanfani F
  • Journal: J Biol Chem
  • PubMed ID: 9083020
Catalog #
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