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          Single cell RT-PCR analysis of ClC-2 mRNA expression in ureteric bud tip. Citations & References

          • Authors: Huber S; Schroppel B; Kretzler M; Schlondorff D; Horster M
          • Journal: The American Journal of Physiology 1998 5:F951-F957
          Catalog #
          • 4318739(Discontinued)
          • 4327058(Discontinued)
          • 4327059(Discontinued)

          Have you tested cells grown in Gibco PSC Neural Induction Medium (NIM) for Scorecard analysis? Product FAQ

          Answer

          H9 cells were differentiated into NSCs using Gibco PSC Neural Induction Medium (NIM) and hPSC Scorecard analysis was performed at various time points. The control sample was undifferentiated H9 ESC. Cells were seen to become positive for ectoderm by day 5.

          Answer Id:: E18589

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          Do you offer the individual components of the Human Neural Stem Cell Immunocytochemistry Kit as standalone products? Product FAQ

          Answer

          Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

          Answer Id:: E19021

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          Genetic analysis of the cell division protein FtsI (PBP3): amino acid substitutions that impair septal localization of FtsI and recruitment of FtsN. Citations & References

          • Authors: Wissel MC, Weiss DS
          • Journal: J Bacteriol (2004) 186:490-502
          • PubMed ID: 14702319
          Catalog #

          Can I use the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

          Answer

          We do not recommend using the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

          Answer Id:: E19022

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          With the direct lysis approach to DNA/RNA analysis, can I use cells that are frozen or stabilized with RNAlater solution? Product FAQ

          Answer

          For the Cells-to-CT lysis reaction, you can use fresh cells, frozen cells, or cells that have been stabilized with RNAlater solution. You just want to ensure that cells were washed once with PBS before going into the lysis reaction.

          Answer Id:: E7108

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          Cancer-testis antigens and ING1 tumor suppressor gene product are breast cancer antigens: characterization of tissue-specific ING1 transcripts and a homologue gene. Citations & References

          • Authors: Jager D; Stockert E; Scanlan MJ; Gure AO; Jager E; Knuth A; Old LJ; Chen YT
          • Journal: Cancer Research 2000 1:6197-6204
          Catalog #
          • 4318739(Discontinued)
          • 4327058(Discontinued)
          • 4327059(Discontinued)

          I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes? Product FAQ

          Answer

          Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

          Answer Id:: E16144

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          Wastewater Analysis Products Product Literature

          What product do you provide for activating mouse T cells? Product FAQ

          Answer

          We offer Dynabeads Mouse T-Activator CD3/CD28 in 3 different volume sizes (Cat. No. 11456D (0.4 mL), Cat. No. 11452D (2 mL), Cat. No. 11453D (5 x 2 mL)). This product is intended for activation of mouse CD4+ or CD8+ T cells, or mouse Treg cells.

          Answer Id:: E12161

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          Brochures & Specifications: Analysis of Gene Expression in Normal and Tumor Liver Tissue: Expression Array System: Data Sheet Product Literature

          What concentration of my antibody should I use for cell analysis? Product FAQ

          Answer

          An optimal concentration may be between 1-10 µg/mL for cell and tissue labeling for microscopy, or 0.2-5 µg/mL for flow cytometry. A range of concentrations should be tested to determine what is optimal.

          Answer Id:: E14774

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          What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation or Western blot analysis? Product FAQ

          Answer

          The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

          Answer Id:: E4434

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          With the direct lysis approach to DNA/RNA analysis, can I use the direct lysis solution for uncultured primary cells or my other cell lines? Product FAQ

          Answer

          We use multiple cells lines in-house, including primary human hepatocytes, stem cells, and differentiated stem cells. When using a new cell line, we recommend that a pilot experiment with a dilution of cells from 10-100,000 cells looking for a loss in linearity of results. Please refer to the manual for how to perform this experiment.

          Answer Id:: E7110

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          BioProbes article: Time-resolved analysis of proteome dynamics by tandem mass tags and stable isotope labeling in cell culture hyperplexing Product Literature

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