Manual / Product Insert

StemPro® Accutase® Cell Dissociation Reagent

Version: MAN0007245 1.00 (14 May 14)
Catalog #

Product Literature

StemPro® Accutase® Cell Dissociation Reagent Brochure

Product FAQ

What reagents do you offer for cell dissociation, and what are the main differences between them?

Answer

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Answer Id: E11900

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Which dissociation reagent should I use with the Gibco PSC Definitive Endoderm Induction Kit?

Answer

We recommend using EDTA for passaging PSCs. For seeding cells for definitive endoderm induction, we recommend using StemPro Accutase Cell Dissociation Reagent for small clumps and Gibco TrypLE Enzyme for singularized cells.

Answer Id: E12479

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What other dissociation reagents have been tested with Gibco Essential 8 Flex Medium?

Answer

Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.

Answer Id: E10277

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

How can I remove non-neural cells during NSC expansion following the induction of NSCs from hPSCs using PSC Neural Induction Medium?

Answer

We recommend using Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No. A11105). The detailed protocol is listed in the PSC Neural Induction Medium manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/psc_neural_induction_medium_man.pdf).

Answer Id: E12544

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What are the benefits of Gibco TrypLE reagent over trypsin?

Answer

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Answer Id: E11901

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What can be used to detach cells from plates when coated with a thin layer of collagen I?

Answer

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

Answer Id: E11806

Was this answer helpful?

Yes
No
Thank you for your response

Manual / Product Insert

User Bulletin: Single cell dissociation of PSCs for cardiomyocyte differentiation

Version: MAN0010978 Rev B.0 (02 April 2015)
Catalog #
  • A25042SA(Discontinued)

Product Literature

Brochure: Essential 8 media systems

Product Literature

Flyer: YLLSoM CCE Promotion Jun-Aug 2019 (SG)

Manual / Product Insert

Reprogramming Fibroblasts with the CytoTune™-iPS Reprogramming Kit

Version: 22 August 2012
Catalog #
  • A1378001(Discontinued)
  • A1378002(Discontinued)
  • 123-LSG

Manual / Product Insert

User Guide: PSC Human Dopaminergic Neuron Differentiation Kit (Prototype)

Version: MAN0014654 Rev 1.0 (18 November 2015)