Cell lysis using detergents Mammalian cell lysis reagents Bacterial protein extraction reagents Insect, yeast and plant cell lysis reagents Recommended reading, Cell Lysis (Total Protein Extraction) Detergents for Protein Solubilization T-PER Tissue Protein Extraction Reagent M-PER Mammalian Protein...

These kits are optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions, including cytoplasmic, membrane, nuclear, chromatin-bound, and cytoskeletal proteins in 1–3 hrs.

Structure and diversity of cells Cell lysis and protein extraction Cell fractionation and organelle isolation Protease inhibition and protein stabilization Protein refolding Recommended reading, Cell Lysis (Total Protein Extraction) Selection Guide Detergents for Protein Solubilization Subcellular...

Coming soon Coming soon Coming soon Coming soon Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like...

Properties and types of detergents Cell membrane structure, protein solubilization and detergents Detergent removal methods Recommended reading, Detergents for Solubilization Protein Purification and Isolation Cell Lysis and Fractionation Protein Dialysis, Desalting, and Concentration Selection...

Our tissue lysis, cell lysis, and cell fractionation products are optimized for sample type. The lysates or fractions collected are compatible with a wide range of downstream applications. Features include: See western blotting resources and products › High protein yields from cells or tissues...

For Research Use Only. Not for use in diagnostic procedures., View all Why are protease/phosphatase inhibitors important during cell lysis? Which protein assay is my lysis compatible with? What lysis buffers do you offer for mammalian cells? Why is cell fractionation important?

Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.

Physical disruption vs. solution-based cell lysis Techniques of protein extraction by physical disruption Additives and methods to facilitate physical disruption Recommended reading, Protein Purification and Isolation Cell Lysis and Fractionation Pierce Tissue Strainers Lysozyme Solution DNase I...

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.

Extract total protein from mammalian tissue or cell samples using mild but efficient lysis reagents. These reagents have been optimized and validated with specific tissue types, as well as in primary and cultured mammalian cells.

Efficiently lyse E. coli and other bacterial cells without mechanical disruption while gently extracting native proteins with these nonionic, all-in-one detergent solutions. Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation that combines a lysis...

Detergents are amphipathic molecules containing both a nonpolar "tail" having aliphatic or aromatic character, and a polar "head." The ionic character of the polar head group forms the basis for broad classification of detergents; they may be ionic (charged, either anionic or cationic), nonionic...

Easily extract, purify, clean up, and concentrate your proteins of interest with our kits, reagents, and devices. These products are optimized for a wide range of tissue and cell types and compatible with a broad range of protein purification resins and formats, which offer more choices and better...

Gram-positive Bacilli , Lactobacilli and Gram-negative E. coli are favorite recombinant protein production systems due to their simple cultivation and easy genetic manipulation. In these prokaryotic recombinant cell factories, the accumulation of target product can be secreted into the cultivation...