Documents & Support

1-15 of 1,491 Results

HCS LipidTOX Phospholipidosis Detection Reagents Manual / Product Insert

  • Pub. No.: a23d7e9e00b404af52c11e1bd0a7f7c337a4f22e
  • Version: 09-01-2006
Catalog #

HCS LipidTOX Phospholipidosis and Steatosis Detection Kits Manual / Product Insert

  • Pub. No.: cec215e2fddb1df2882640e72e0dab672ddcb37e
  • Version: 09-01-2006
Catalog #

I'm seeing aggregates in my thawed LipidTox stain solutions. Is this okay? Product FAQ

Answer

After thawing LipidTOX Red phospholipidosis detection reagent, some minute aggregates might be observed in the solution. They usually will disappear if the vial is incubated in a 37 degrees C water bath for 5 minutes. These aggregates do not affect the performance of the assay.
Any aggregates that remain after the stain is diluted in media are removed by 0.2 µm filtration before the labeling solution is added to the cells, as recommended in the protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E10100

Was this answer helpful?

Yes No

Thank you for your response

How do the specific parameters on an objective relate to my application and how do I select the right one for my EVOS imaging system? Product FAQ

Answer

Please find a comprehensive objective selection guide in the link below. It provides detailed guidance based on sample type, vessel type and mode of imaging.
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/cell-imaging-systems/evos-objectives/selection-guide-evos-objectives.html

Answer Id: E19176

Was this answer helpful?

Yes No

Thank you for your response

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains? Product FAQ

Answer

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E9822

Was this answer helpful?

Yes No

Thank you for your response

HCS LipidTOX Neutral Lipid Stains Manual / Product Insert

  • Pub. No.: b166ba705477321572fc6f0e9b3c1c3411140e08
  • Version: 09-01-2006
Catalog #

Which dyes can I use on the FLoid Cell Imaging Station? Product FAQ

Answer

The FLoid Cell Imaging Station contains the three most common channels used in fluorescence imaging: DAPI, Alexa Fluor 488 / GFP/ FITC, and Texas Red channel. For a complete list of Molecular Probes dyes that are compatible with the device, please see the FLoid Cell Imaging Station Dye compatibility page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/cell-imaging-systems/floid-cell-imaging-station/floid-reagent-dye-compatibility.html).

Answer Id: E18428

Was this answer helpful?

Yes No

Thank you for your response

For high-content analysis (HCA)/high-content screening (HCS), what are the main differences between the CellInsight CX5, CX7, and ArrayScan systems? Product FAQ

Answer

The basic differences are in the number of channels for excitation (5 versus 7), the ability to image in brightfield, widefield, and confocal, and the number of positions available on the objective turret. The CellInsight CX5 System is the most basic HCS system with illumination in 5 channels, imaging in brightfield and widefield, and a 1-position objective turret. The CellInsight CX7 System and ArrayScan System provide illumination in 7 channels, imaging in brightfield, widefield, and confocal, and either a 3-position (CX7) or 4-position objective turret (ArrayScan System). The ArrayScan System has the added option of live-cell imaging. For more information, go here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/high-content-screening.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14927

Was this answer helpful?

Yes No

Thank you for your response

I want to mount my dye-labeled cells in an antifade mounting medium to keep the dyes from photobleaching. Which mounting medium do you recommend? Product FAQ

Answer

As dyes are illuminated for imaging, they will fade, or “photobleach”, leading to unwanted dimming and lower detection efficiency over time. An antifade mounting medium can greatly reduce photobleaching. If you wish to label live cells, use of ProLong Live Antifade Reagent is helpful. If you wish to mount fixed cells after labeling, and then image immediately and then discard, SlowFade Diamond Antifade Mountant stays liquid but has good refractive index. If you wish to mount your sample and then archive the slides, ProLong Diamond Antifade Mountant will harden to a better refractive index and allow for archiving of the sample for up to several weeks, or even months. Unlike other antifade mounting media, these work well with fluorescent proteins for immediate viewing (archiving fluorescent proteins is not possible), and they are packaged with or without DAPI. More information on these can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-gold-antifade.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14931

Was this answer helpful?

Yes No

Thank you for your response

My fluorescent dye signal is fading as I image it. What can I do to stop this? Product FAQ

Answer

All fluorescent dyes will fade, or “photobleach”, to at least some extent when exposed to strong light at the wavelengths they absorb. Here are some causes for photobleaching and ways to fix the problem:

1) Cause of photobleacing - Generation of free radicals and singlet oxygen
Remedy - i) Use an antifade reagent, which has antioxidants and free radical scavengers:

ii) For live-cell imaging of fluorescent dyes and proteins, we recommend ProLong Live Antifade Reagent which can be added to the cell media or buffer. ProLong Live Antifade Reagent can significantly increase the stability over time for reagents as well as fluorescent proteins, like GFP, without affecting cell health, for up to 24 hours.

iii) For immediate analysis and short-term storage of fixed samples, we recommend SlowFade Diamond Antifade Mountant (it is a liquid mountant and can be used for immediate viewing and then disposal of the sample within a day).

iv) For long-term analysis of Alexa Fluor dyes in fixed samples, we recommend a mountant that hardens, such as ProLong Diamond Antifade Mountant. The harrdening of the mountant also slows diffusion of free radicals).

v) For long-term analysis of all dyes and fluorescent proteins in fixed samples, we recommend ProLong Diamond Antifade Mountant, suitable for archiving slides.

2) Cause of photobleaching - Dye is particularly sensitive to structural modification upon exposure to light.
Remedy - i) Choose a more photostable dye, such as many of our Alexa Fluor dyes.

3) Cause of photobleaching - Intense Illlumination
Remedy - i) Reduce light exposure, for example by reducing laser power or using neutral density filters.

ii) Minimize the viewing time of labeled sample, and close shutter when not viewing.

iii) Use an objective with a lower numerical aperture, such as a lower-power objective.

You can find more information on choosing an antifade reagent on the link below http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades.html.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14792

Was this answer helpful?

Yes No

Thank you for your response

Application Note: Simple and accurate monitoring of adipogenesis Product Literature

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use? Product FAQ

Answer

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14778

Was this answer helpful?

Yes No

Thank you for your response

I am seeing non-specific binding in the nuclei and mitochondria of my cells with a secondary-only control, but protein binding isn't enough to stop it. What can I do? Product FAQ

Answer

What may be happening is non-specific binding of the secondary antibody due to dye charge, for example, where the negatively-charged dye is attracted to positively-charged cellular components. To block this, use Image-iT FX Signal Enhancer (Cat Nos. I36933 and R37107), which blocks non-specific binding due to charge interactions between the dyes on conjugates and cellular components.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E14786

Was this answer helpful?

Yes No

Thank you for your response

Sphingosine-1-Phosphate 3 (S1P3) Receptor Redistribution Assay - Instructions Product Literature

Cannabinoid Receptor 2 (CB2) Redistribution Assay - Instructions Product Literature

Results per page