Cells should be pelleted by centrifugation at 180 x g (relative centrifugal force, RCF). The correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found at this website (https://www.eppendorf.com/US-en/Products/Special/Webtools/centrifuge-speed-calculator/). For example, to centrifuge cells at 180 x g (RCF) in a rotor with a radius of 15 cm, you would spin the cells at 1,036 rpm.
Answer Id: E11968
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