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Gibco Ham's F-12 Nutrient Mixture was formulated for single-cell plating of near-diploid Chinese Hamster Ovary (CHO) cells. Find glutamine-supplemented F-12 media as well GlutaMAX supplemented formulations, Kaighn's F-12 formulation, and powdered formats.

Gibco Ham's F-10 Nutrient Mixture Media is formulated for serum-free growth Chinese Hamster Ovary (CHO) cells and the growth of various mammalian cell lines with serum supplementation. Rely on Gibco Ham's F-10 Nutrient Mixture, formulated for superior quality, greater reliability and improved...

The culture medium is the most important component of the culture environment, because it provides the necessary nutrients, growth factors, and hormones for cell growth, as well as regulating the pH and the osmotic pressure of the culture.

The first step in the recombinant biotherapeutic downstream process is harvesting cell culture fluid containing the product. Conventional approaches for performing this unit operation utilize a combination of depth filtration, centrifugation, and membrane filtration.

The production of therapeutic antibodies and proteins is one of the fastest growing sectors of the pharmaceutical industry, where accuracy and consistency in bioprocessing can pose challenges. From the first discovery of insulin to the most recent ground-breaking therapeutics, we lead the way in...

Gibco bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three...

Several new kits for streamlining protein generation in CHO DG44 (dhfr¯) cells are now available. The cells, media, and transfection reagents are designed for suspension culture, reducing the time needed to get to expressed protein.

A revolutionary additive for Chinese hamster ovary (CHO) fed-batch processes. Up to double titer Highly concentrated, pH-neutral additive Minimal optimization required, Fast, flexible performance in fed-batch cell culture.

Specially designed cationic lipids, such as the Invitrogen Lipofectamine Transfection Reagents, facilitate DNA and siRNA delivery into cells (Chesnoy and Huang, 2000; Hirko et al., 2003; Liu et al., 2003).

, Podcast Summary: In this podcast, Dr. Henry Chiou, Director of Protein and Viral Expression Systems and Transfection at Thermo Fisher Scientific, discusses how transient protein expression systems are enabling rapid and high-yield production of proteins for applications such as vaccine and...

We offer transfection reagents for DNA, siRNA, RNA, and protein delivery, providing a range of options to best suit your transfection experiment: When selecting a transfection method, consider the payload you wish to deliver (DNA, RNA, or protein) and the type of cells you want to transfect.

The pcDNA™3.3-TOPO® TA Mammalian Expression Vector enables expression of exceptionally high levels of recombinant protein in adherent-or suspension-adapted mammalian cells. Obtain up to five-fold higher protein yields Fast, simple, high efficient TOPO® cloning saves time Express native proteins,...

Thermo Scientific Nunc flasks, dishes, and plates feature the standard Nunclon Delta cell culture–treated surface for growing adherent cells. Every lot of these cell culture products is tested and certified for cell growth and plating efficiency using 4 different cell types: L-929, HEL 299, V79‑4,...

An optimized expression system for CHO and 293 suspension cultures Functional proteins with mammalian posttranslational modifications Easy scale-up and purification with serum-free and animal origin–free components, The FreeStyle MAX System is a breakthrough protein production technology for easily...

Cell-Based Assays in Virology Research Bioassay Cell Line Development Next Step: Animal Model Development From Basic Research to Drug Discovery , To unravel the complexity of the viral life cycle, researchers must rely on model and assay systems to efficiently explore mechanisms that drive...