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Chromatography Columns and Consumables Catalog – Gas Chromatography Product Literature

2019/2021 Chromatography Consumables Catalog - Liquid Chromatography Product Literature

RF1971 SDS

RF2796 SDS

Catalog #

What formats are available for the Melon Gel Support? Product FAQ

Answer

There are three different formats. The Melon Gel IgG Purification Kit (Cat. No. 45212) and Melon Gel IgG Spin Purification Kit (Cat. No. 45206) are for purifying antibodies from serum. The Melon Gel Monoclonal IgG Purification Kit (Cat. No. 45214) is for purifying monoclonal antibodies from cell-culture supernatant and ascites fluid. Ready-to-use prepacked chromatography cartridges are available as two 1 mL columns (Cat. No. 89932) or one 5 mL column (Cat. No. 89933).

Answer Id:: E8640

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RF1062 SDS

Catalog #

What is the difference between HisPur Nickel Resin and HisPur Cobalt Resin? Product FAQ

Answer

Nickel has a higher affinity for histidines than does cobalt. It binds multiple histidines more tightly than cobalt, and requires more stringent conditions than is necessary for cobalt. The lower affinity of cobalt for multiple histidines typically results in less nonspecific binding of histidine-rich proteins that lack a his-tag compared to nickel resins.

HisPur Ni-NTA resin has a high capacity of up to 60 mg of 6xHis-tagged protein per milliliter. It is a versatile resin that can work under both native and denaturing conditions, and can also be used with a variety of lysis reagents and buffer additives.

HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties.

Answer Id:: E12933

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RF1162 SDS

Catalog #

RF2154 SDS

Catalog #

Synergistic activation of eIF4A by eIF4B and eIF4G Citations & References

  • Authors: Nielsen K. H. et al.
  • Journal: Nucleic Acids Res
  • PubMed ID: 0

Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-{zeta} and PP2A pathway Citations & References

  • Authors: Caraballo J. C. et al.
  • Journal: Am J Physiol Lung Cell Mol Physiol
  • PubMed ID: 0

Preferential accumulation of N-terminal mutant huntingtin in the nuclei of striatal neurons is regulated by phosphorylation Citations & References

  • Authors: Havel L. S. et al.
  • Journal: Hum Mol Genet
  • PubMed ID: 0

Mechanism of ATP turnover inhibition in the EJC Citations & References

  • Authors: Nielsen K. H. et al.
  • Journal: RNA
  • PubMed ID: 0

Targeted peptidecentric proteomics reveals caspase-7 as a substrate of the caspase-1 inflammasomes Citations & References

  • Authors: Lamkanfi M. et al.
  • Journal: Mol Cell Proteomics
  • PubMed ID: 0

2019/2021 Chromatography Consumables Catalog - Sample Preparation Product Literature

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