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How many data sources were used for the Clariom S array? Product FAQ

Answer

We used 15 databases to design the Clariom S array. These databases are listed in the Clariom S solutions data sheet that can be found on the Clariom S assay, mouse product page, under Documents > Product Literature: https://www.thermofisher.com/order/catalog/product/902930?SID=srch-srp-902930#/902930.

Answer Id:: E18719

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Why is strand-specificity important when performing Clariom D and Clariom S assays? Product FAQ

Answer

Clariom D arrays have probes that cover all known regions of transcription including probes in overlapping regions from both strands. To obtain strand-specific information from the Clariom D arrays, the WT Pico and WT Plus reagents (which are strand-specific) must be used. This is important because without strand-specific reagent, it would not be possible to decipher the source strand of DNA, which makes it challenging to untangle true gene- and exon- level expression and alternative splicing events.

Strand-specificity is significantly less important for customers interested in gene-level only information (i.e., those using Clariom S) as compared to customers who want to understand the complexities of the whole transcriptome including identifying antisense transcripts and ncRNA (i.e., those using Clariom D). While strand-specificity is less important for gene-level expression only, probes within regions of overlapping transcription from both strands are avoided in the Clariom S array design (unlike Clariom D). This is important because if Clariom S did not preserve strand-specificity, there could be an overestimation of gene-level expression causing false positive or negative results. With Clariom S having a "stranded" design, it does not necessarily need a strand-specific reagent kit.

Answer Id:: E18132

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