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Sample Data: Clariom S Assay Human HT Support Files

  • Version: Jan. 2017
  • Size: 16.5 MB

Sample Data: Clariom S Assay Mouse HT Support Files

  • Version: Jan. 2017
  • Size: 12.7 MB

Why is strand-specificity important when performing Clariom D and Clariom S assays? Product FAQ

Answer

Clariom D arrays have probes that cover all known regions of transcription including probes in overlapping regions from both strands. To obtain strand-specific information from the Clariom D arrays, the WT Pico and WT Plus reagents (which are strand-specific) must be used. This is important because without strand-specific reagent, it would not be possible to decipher the source strand of DNA, which makes it challenging to untangle true gene- and exon- level expression and alternative splicing events.

Strand-specificity is significantly less important for customers interested in gene-level only information (i.e., those using Clariom S) as compared to customers who want to understand the complexities of the whole transcriptome including identifying antisense transcripts and ncRNA (i.e., those using Clariom D). While strand-specificity is less important for gene-level expression only, probes within regions of overlapping transcription from both strands are avoided in the Clariom S array design (unlike Clariom D). This is important because if Clariom S did not preserve strand-specificity, there could be an overestimation of gene-level expression causing false positive or negative results. With Clariom S having a "stranded" design, it does not necessarily need a strand-specific reagent kit.

Answer Id:: E18132

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Sample Data: Clariom S Assay Rat HT Support Files

  • Version: Jan. 2017
  • Size: 14.7 MB

Product Inclusion List 2020, KingFisher Trade In Product Literature

What are the available assay options for running the Clariom S array? Product FAQ

Answer

Depending on input, sample type, and platform, you have several options:
Cartridge: Assays for 100 pg-50 ng of total RNA isolated from whole blood, cultured cells, and fresh/fresh frozen or FFPE tissues.
Pico Assay Assays for 50-500 ng of total RNA isolated from whole blood, cultured cells, and fresh/fresh frozen tissues.
WT Plus Assay Plates: Assays for 100 pg-50 ng of total RNA isolated from whole blood, cultured cells, and fresh/fresh frozen or FFPE tissues for analysis on GeneTitan Instrument.
Pico Assay HT Assays for 50-500 ng of total RNA isolated from whole blood, cultured cells, and fresh/fresh frozen tissues for analysis on GeneTitan Instrument.

Answer Id:: E14312

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What are the Clariom Assay scan times and file sizes? Product FAQ

Answer

Clariom D Assay - scan time is about 33 min.
- DAT file size about 790-800MB
- CEL file size about 68 MB

The Clariom S - scan time is about 5 min.
- DAT file size about 35-40MB
- CEL file size about 3 MB

Answer Id:: E14314

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User Guide: Clariom GO Screen Assay 384-Array Format Workflow Manual / Product Insert

  • Version: B.0
Catalog #

Is globin reduction of blood samples required when using the Clariom S arrays? Product FAQ

Answer

No, there is no need to globin reduce your samples prior to starting the assay for the Clariom S array.

Answer Id:: E14313

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Biomarker Quantitation Assay Guide Product Literature

Laboratory Product Checklist - Protein, RNA, cell, and DNA Product Literature

Presentation: Cells-to-Signal User Guidance for Clariom GO Screen sample preparation Product Literature

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