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Can any Gibco formulation be customized with the Gibco Media Configurator? Product FAQ

Answer

Almost all Gibco cell culture basal media products can be customized using the Gibco Media Configurator. To check on a particular product, use the Media Configurator page (https://www.thermofisher.com/us/en/home/life-science/bioproduction/custom-cell-culture-media-and-services/gibco-custom-media-configurator.html to search by Gibco catalog number, or browse our offering by description. For the customization of non-media Gibco products, such as buffers and growth factors, contact us at custommedia@lifetech.com.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11844

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Cell Culture Media and Reagent Product Literature

What type of cells can I culture with Gibco Human Plasma-Like Medium (HPLM)? Product FAQ

Answer

Most cell lines that are cultured using classic synthetic cell culture media, including MEM, DMEM, RPMI 1640, and DMEM/F-12 can be cultured with Gibco HPLM. There might be some differences in growth rates for some cell lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E19387

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Cell Culture Media - MarrowMAX Product Literature

Will Gibco Human Plasma-Like Medium (HPLM) work on primate cells? Product FAQ

Answer

We have not specifically tested primate cells with Gibco HPLM. If the primate cells are grown using classic synthetic cell culture media, including MEM, DMEM, RPMI 1640, and DMEM/F-12, they should grow in Gibco HPLM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E19392

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How much serum is in Opti-MEM I Medium? Product FAQ

Answer

There is no serum in Opti-MEM I Medium. The term "reduced serum" means that one can reduce the amount of serum that is used with classical cell culture media. When working with Opti-MEM I medium, most cells that are routinely cultured in serum-supplemented medium may be transferred directly into Opti-MEM I medium with a minimum of 50% reduction in serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E2993

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What is the difference between Gibco Human Plasma-Like Medium (HPLM) and other synthetic media? Product FAQ

Answer

The widely used classic synthetic cell culture media, including MEM, DMEM, RPMI 1640, and DMEM/F-12 contain glucose, amino acids, vitamins, and salts at concentrations that in large part do not reflect those found in human plasma. These media also lack additional components needed to mimic the metabolic profile of human plasma. Gibco HPLM contains more than 60 polar metabolites such as amino acids, nucleic acids, sugars, and small organic acids at concentrations found in human plasma. The salt concentrations in HPLM mimic the salt concentrations found in human plasma.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E19380

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What are Gibco Cell Culture Bags? Product FAQ

Answer

Gibco Cell Culture Bags are convenient, pre-filled bags in a variety of formulations and sizes, specifically designed to increase productivity and reduce costs. The bags can be formatted up to 1,000 L. Read more about these bags here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11839

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How much serum should I add to my media? Product FAQ

Answer

The serum concentration will vary with the cell line and basal medium used. Please go here to see our recommended sera supplementations for tested cell lines (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/advanced-d-mem-and-mem/recommended-sera-supplementations.html).

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Answer Id: E11862

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Are there any publications with Human Plasma-Like Medium (HPLM)? Product FAQ

Answer

There are several publications using HPLM. A list of select publications using HPLM can be found on the HPLM web page: https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/human-plasma-like-medium.html

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E19400

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Is degradation of BenchStable media due to light exposure only limited to fluorescent light? Product FAQ

Answer

Our in-house data, using refrigerated BenchStable media exposed to fluorescent light (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/benchstable-cell-culture-media.html#results), illustrates the necessity of having light protection. While we have not tested other light spectrums; we recommend storing BenchStable media protected from any light exposure.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E17931

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What is the Gibco PE Film? Product FAQ

Answer

Based on feedback from customers, we designed a media bag portfolio that uses a consistent film for all bag sizes. This film, called PE film, is superior in function to other commercially available films. Please go here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11840

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Does Gibco Human Plasma-Like Medium (HPLM) need to be supplemented with FBS? Product FAQ

Answer

Complete cell culture media most often consist of a basal medium containing defined metabolites and salts supplemented with fetal bovine serum (FBS) that further contributes an undefined cocktail of growth factors, hormones, trace elements, and other components critical for cell growth. To establish a complete HPLM-based medium, supplementation with 10% dialyzed FBS (Cat. No. 26400044) is recommended in order to minimize the contribution of polar metabolites at otherwise undefined levels. Untreated FBS can be also be manually dialyzed by using SnakeSkin dialysis tubing, 3.5K MWCO (Cat. No. 88242) as an alternative to purchasing dialyzed FBS. If your specific application is not impacted by the levels of polar metabolites present in serum, or if your cells do not tolerate dialyzed serum, standard or heat-inactivated FBS can be used in place of dialyzed FBS.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Answer Id: E19382

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What are the benefits of the Gibco PE film? Product FAQ

Answer

The commercially available film is a very clear, multi-layered, single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250 mL to 1,000L). In addition, the film is a market leader with exceptional gas barrier properties and very low extractable and leachable results. Click here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11841

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What aspects of cell culture media can be customized? Product FAQ

Answer

The Gibco Media Configurator allows you to add, remove, and adjust the concentration of components. In addition, you can select from a range of packaging, QC tests, and either cGMP or non-cGMP manufacturing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11845

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