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LabAid: CloneJET PCR Cloning Kit Product Literature

What is the storage media for the competent cells? Product FAQ

Answer

Preparation procedures and formulations for all of our competent cells are proprietary. All chemically competent cells are delivered in an aqueous solution that contains a mixture of salts, along with a freezing stabilizer such as glycerol or DMSO.

Answer Id:: E6719

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Lot # 2337903 Certificate

Catalog # EC0113

Lot # 2121671 Certificate

Catalog # EC0113

Lot # 2284983 Certificate

Catalog # EC0113

Lot # 2205135 Certificate

Catalog # EC0113

What is the formulation of the SOC medium that is provided with competent cells? Product FAQ

Answer

SOC (Super Optimal Catabolite) Medium Preparation (for 1 Liter):

1) To a 2 Liter flask with stir bar add the following:
- Bacto Tryptone 20 g
- Yeast Extract 5 g
- Sodium Chloride (NaCl) 0.58 g
- Potassium Chloride (KCl) 0.186 g
2) Add sterile water to a final volume of 1 Liter.
3) Mix well on magnetic stir plate for 5-10 minutes or until all of the ingredients are well mixed and completely dissolved.
4) Autoclave 30 minutes.
5) Allow to cool to room temperature.
6) Add 10 ml of sterile 2M Magnesium Solution (1M Magnesium sulfate, 1M Magnesium chloride)and mix well.
7) Add 10 ml of sterile 2M Glucose and mix well. (Final Glucose concentration is 20 mM).

Answer Id:: E3343

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Flyer: Confidently Competent - Thermo Scientific Competent Cells Product Literature

How can I obtain the highest possible transformation efficiency with Thermo Scientific DH5α and DH10B competent cells? Product FAQ

Answer

To achieve the highest possible transformation efficiency with Thermo Scientific DH5α (Cat. No. EC0112) and DH10B (Cat. No. EC0113) competent cells, please follow the transformation protocol provided with the product and pay attention to the following details:

- Make sure to use wet well-pressed ice to maximize cold surface volume in all the transformation steps.
- Perform the steps quickly and accurately.
- Use chilled microcentrifuge tubes and bury the whole tube up to the cap in the wet ice.
- Do not exceed the recommended heat shock time and transfer the tube with cells back to the wet ice immediately.

Answer Id:: E17335

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Lot # 1977237 Certificate

Catalog # C4081201

Lot # 2000928 Certificate

Catalog # C4081201

Lot # 2340934 Certificate

Catalog # C4081201
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