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How long is Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) functional group (maleimide) stable for after the amine targeted end is occupied? Product FAQ

Answer

The Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) maleimide group is stable for 64 hours in 0.1 M sodium buffer, pH 7 at 4 degrees C.

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Answer Id: E21954

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Is the cyclohexane ring in Sulfo-SMCC crosslinker cis or trans? Product FAQ

Answer

The cyclohexane ring in Sulfo-SMCC crosslinker is trans.

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Answer Id: E13360

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Can you provide the shelf-life for Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)? Product FAQ

Answer

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

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Answer Id: E19845

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What is the difference between SMCC and Sulfo-SMCC? Product FAQ

Answer

SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). Sulfo-SMCC is a similar compound but it has a sulfonate group on the NHS ring which makes it water-soluble whereas SMCC is water-insoluble and needs to be dissolved first in an organic solvent like DMF or DMSO. Subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

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Answer Id: E16238

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What is the shelf life of Sulfo-SMCC (Cat. No. 22322)? Product FAQ

Answer

Sulfo-SMCC is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

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Answer Id: E19744

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User Guide: SMCC and Sulfo-SMCC Manual / Product Insert

  • Version: C.0
Catalog #

We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible? Product FAQ

Answer

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

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Answer Id: E15731

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Can you provide the shelf-life for SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)? Product FAQ

Answer

SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

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Answer Id: E19882

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Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer? Product FAQ

Answer

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.

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Answer Id: E13381

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Can you provide the shelf-life for LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate)))? Product FAQ

Answer

LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Answer Id: E19883

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What is the expiration date for the Sulfo-SMCC crosslinker? Product FAQ

Answer

This product has no set expiration date. We monitor the bulk material in such a manner that allows us to warranty the product for one year from the date of shipment. It may still be usable beyond the one year if handled properly. However, using material older than one year is typically not recommended since the material easily hydrolyzes upon exposure to moisture.

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Answer Id: E13347

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Can I make up a Sulfo-SMCC stock solution and store the cross-linker long-term? Product FAQ

Answer

Long-term storage is not recommended since the NHS-ester group is susceptible to hydrolysis. Hydrolysis begins upon reconstitution. Even organic solvents such as DMSO and DMF are hygroscopic, as a result, they tend to absorb water, which in turn also promotes the hydrolysis of the NHS-Ester.

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Answer Id: E13350

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Can Sulfo-SMCC crosslinker reagent be reconstituted in an organic solvent (DMF or DMSO) and added to an aqueous reaction mixture? Product FAQ

Answer

Yes, Sulfo-SMCC can be reconstituted in an organic solvent (DMF or DMSO) and added to an aqueous reaction mixture.

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Answer Id: E13359

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Can the Sulfo-SMCC cross-linking agent be added directly to the reaction mixture? Product FAQ

Answer

Yes, Sulfo-SMCC can be added directly to the reaction mixture.

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Answer Id: E13357

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What does "store under nitrogen" mean for Sulfo-SMCC crosslinker? Product FAQ

Answer

A gentle stream of nitrogen gas is held over the opening of the vial to flush the oxygen from the vial. The cap is then immediately placed on the vial. If storing under nitrogen, caution should be taken to ensure that the stream of nitrogen is gentle enough, so that the powdered product will not be blown out of the bottle. If nitrogen is not available, weigh the material as quickly as possible and immediately cap. Store desiccated.

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Answer Id: E13349

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