Product Literature

Application Note: Evaluation of the toxic effects of PFOS in hESCs differentiating into cardiomycytes

Product Literature

Application Note: Enhanced expansion of neural stem cells using a heat stable bFGF

Product Literature

Brochure: BenchStable Media

Manual / Product Insert

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Version: MAN0007332 1.00 (16Jan2014)

Citations & References

Prostaglandin FP agonists alter metalloproteinase gene expression in sclera.

  • Authors: Weinreb RN; Lindsey JD; Marchenko G; Marchenko N; Angert M; Strongin A
  • Journal: Investigative Ophthalmology & Visual Science 2005 12:4368-4377

Product Literature

Application Note: Characterization of cancer-associated IDH mutations in GeneArt Engineered Cell Models

Manual / Product Insert

User Guide: PSC Human Dopaminergic Neuron Differentiation Kit

Version: MAN0015919 Rev 1.0 (01 June 2016)

Citations & References

The cystic fibrosis mutation G551D alters the non-Michaelis-Menten behavior of the cystic fibrosis transmembrane conductance regulator (CFTR) channel and abolishes the inhibitory Genistein binding site.

  • Authors: Derand R, Bulteau-Pignoux L, Becq F,
  • Journal: J Biol Chem (2002) 277:35999-36004
  • PubMed ID: 12124395

Product Literature

Application Note: Superior performance for today’s most challenging stem cell applications—Summary of reprogramming, adaptation, differentiation, and gene editing applications

Manual / Product Insert

StemPro Neural Supplement

Version: MAN0007330 1.00 (12 May 14)
Catalog #

Manual / Product Insert

User Guide: StemPro NSC SFM

Version: 2.0
Catalog #

Product Literature

Application Note: Genome editing of pluripotent stem cells cultured in StemFlex Medium via electroporation and lipid-based transfection

Manual / Product Insert

User Guide: PSC Human Dopaminergic Neuron Differentiation Kit (Prototype)

Version: MAN0014654 Rev 1.0 (18 November 2015)

Product Literature

Novel Alkaline Phosphatase Live Stain to Identify and Isolate Emerging iPSCs

Citations & References

Mitotic-like tau phosphorylation by p25-Cdk5 kinase complex.

  • Authors: Hamdane M, Sambo AV, Delobel P, Bégard S, Violleau A, Delacourte A, Bertrand P, Benavides J, Buée L,
  • Journal: J Biol Chem (2003) 278:34026-34034
  • PubMed ID: 12826674

Manual / Product Insert

StemPro® hESC SFM

Version: MAN0000744 2.00 (5 June 14)
Catalog #

Manual / Product Insert

Rat Fetal NSCs

Version: 7 April 2009

Product Literature

Independent studies demonstrate improved growth characteristics for hESCs in DMEM/F-12 Low Osmolality medium

Product FAQ

Can I use other versions of DMEM/F-12 in place of the Gibco Essential 8 Basal Medium supplied with the kit?

Answer

Other catalog versions of DMEM/F-12 cannot be used in place of the Gibco Essential 8 Basal Medium in the preparation of Gibco Essential 8 Medium. Gibco Essential 8 Basal Medium supplied with the kit has a higher level of sodium bicarbonate.

Answer Id: E12430

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Product FAQ

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Answer

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Answer Id: E13291

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Product FAQ

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

Answer

We suggest storing the supplemented media at 28 degrees C and using it within 2-4 weeks.

Answer Id: E13292

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Product FAQ

How can I prevent poor or dim staining when using Alkaline Phosphatase Live Stain?

Answer

Serum or serum replacement components in the growth medium may cause background and this can result in poor or dim staining. After the removal of the growth medium, gently wash the culture with pre-warmed DMEM/F-12 (Cat. No. 10565-018) for 5 minutes. Aspirate and repeat once before adding the AP Live Stain. To further decrease background staining, perform 3 separate washes of 5 minutes each with DMEM/F-12 for a total of 15 minutes following the staining, and visualize immediately.

Answer Id: E12574

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Product FAQ

How do I visualize cells stained with Alkaline Phosphatase Live Stain?

Answer

After 20-30 minutes, remove the AP Live Stain and wash the cells twice for 5 minutes each with DMEM/F-12 (Cat. No. 10565-018). Following the final wash, add fresh DMEM/F-12 and visualize the stained colonies under fluorescent microscopy using a standard FITC filter. Images should be captured within 10 to 30 minutes following the removal of the dye and the most robust fluorescent colonies should be marked for selection and expansion. Since the fluorescent signal leeches out of the cells and into the surrounding medium as the stain is turned over, we strongly encourage that you visualize and capture images immediately following the final wash for optimal signal detection.

Answer Id: E12567

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Product FAQ

How many times do I need to wash my cells during the Alkaline Phosphatase Live Stain staining procedure?

Answer

There are two washing steps in the staining procedure. First, the cells are washed twice after the removal of the growth medium. Then, after the cells are stained and the dye is removed, the cells are washed twice to eliminate the excess AP Live Stain and reduce the background signal. Washing should be performed gently with pre-warmed basal medium, such as DMEM/F-12 (Cat. No. 10565-018). Handle the cells aseptically, and carefully add and remove the medium with minimal disruption to the adherent cells during this step to avoid damage to cells.

Answer Id: E12564

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