Manual / Product Insert

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Version: MAN0007332 1.00 (16Jan2014)

Manual / Product Insert

User Guide: StemPro NSC SFM

Version: 2.0
Catalog #

Manual / Product Insert

User Guide: PSC Human Dopaminergic Neuron Differentiation Kit (Prototype)

Version: MAN0014654 Rev 1.0 (18 November 2015)

Product Literature

Application Note: Superior performance for today’s most challenging stem cell applications—Summary of reprogramming, adaptation, differentiation, and gene editing applications

Product Literature

Application Note: Enhanced expansion of neural stem cells using a heat stable bFGF

Product Literature

Novel Alkaline Phosphatase Live Stain to Identify and Isolate Emerging iPSCs

Citations & References

Prostaglandin FP agonists alter metalloproteinase gene expression in sclera.

  • Authors: Weinreb RN; Lindsey JD; Marchenko G; Marchenko N; Angert M; Strongin A
  • Journal: Investigative Ophthalmology & Visual Science 2005 12:4368-4377

Product Literature

Application Note: Characterization of cancer-associated IDH mutations in GeneArt Engineered Cell Models

Citations & References

The cystic fibrosis mutation G551D alters the non-Michaelis-Menten behavior of the cystic fibrosis transmembrane conductance regulator (CFTR) channel and abolishes the inhibitory Genistein binding site.

  • Authors: Derand R, Bulteau-Pignoux L, Becq F,
  • Journal: J Biol Chem (2002) 277:35999-36004
  • PubMed ID: 12124395

Product Literature

Brochure: BenchStable Media

Manual / Product Insert

StemPro Neural Supplement

Version: MAN0007330 1.00 (12 May 14)
Catalog #

Citations & References

Mitotic-like tau phosphorylation by p25-Cdk5 kinase complex.

  • Authors: Hamdane M, Sambo AV, Delobel P, Bégard S, Violleau A, Delacourte A, Bertrand P, Benavides J, Buée L,
  • Journal: J Biol Chem (2003) 278:34026-34034
  • PubMed ID: 12826674

Product Literature

Application Note: Genome editing of pluripotent stem cells cultured in StemFlex Medium via electroporation and lipid-based transfection

Manual / Product Insert

Rat Fetal NSCs

Version: 7 April 2009

Manual / Product Insert

StemPro® hESC SFM

Version: MAN0000744 2.00 (5 June 14)
Catalog #

Product Literature

Application Note: Evaluation of the toxic effects of PFOS in hESCs differentiating into cardiomycytes

Product Literature

Independent studies demonstrate improved growth characteristics for hESCs in DMEM/F-12 Low Osmolality medium

Product FAQ

Can I use other versions of DMEM/F-12 in place of the Gibco Essential 8 Basal Medium supplied with the kit?

Answer

Other catalog versions of DMEM/F-12 cannot be used in place of the Gibco Essential 8 Basal Medium in the preparation of Gibco Essential 8 Medium. Gibco Essential 8 Basal Medium supplied with the kit has a higher level of sodium bicarbonate.

Answer Id: E12430

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Product FAQ

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Answer

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Answer Id: E13291

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Product FAQ

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

Answer

We suggest storing the supplemented media at 28 degrees C and using it within 2-4 weeks.

Answer Id: E13292

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Product FAQ

How do I add Alkaline Phosphatase Live Stain to my cells?

Answer

Do not add the concentrated 500X stock solution directly to the dish. AP Live Stain must be diluted 1:500 in basal medium, such as DMEM/F-12 (Cat. No. 10565-018). Prior to adding AP Live Stain, the growth medium must be removed, and the cells must be gently washed twice with pre-warmed basal medium. The 1X AP Live Stain solution is then added directly onto the adherent cell culture. Recommended volumes for preparing the 1X AP Live Stain working solution are given in the manual.

Answer Id: E12563

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Product FAQ

How many times do I need to wash my cells during the Alkaline Phosphatase Live Stain staining procedure?

Answer

There are two washing steps in the staining procedure. First, the cells are washed twice after the removal of the growth medium. Then, after the cells are stained and the dye is removed, the cells are washed twice to eliminate the excess AP Live Stain and reduce the background signal. Washing should be performed gently with pre-warmed basal medium, such as DMEM/F-12 (Cat. No. 10565-018). Handle the cells aseptically, and carefully add and remove the medium with minimal disruption to the adherent cells during this step to avoid damage to cells.

Answer Id: E12564

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Product FAQ

What alternative do you offer for the discontinued SensiCell DMEM/F-12?

Answer

The alternative product is Advanced DMEM/F-12 (Cat. No. 12634-010). Please add 2.5 mM GlutaMAX Supplement to the medium.

Answer Id: E13254

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Product FAQ

How do I visualize cells stained with Alkaline Phosphatase Live Stain?

Answer

After 20-30 minutes, remove the AP Live Stain and wash the cells twice for 5 minutes each with DMEM/F-12 (Cat. No. 10565-018). Following the final wash, add fresh DMEM/F-12 and visualize the stained colonies under fluorescent microscopy using a standard FITC filter. Images should be captured within 10 to 30 minutes following the removal of the dye and the most robust fluorescent colonies should be marked for selection and expansion. Since the fluorescent signal leeches out of the cells and into the surrounding medium as the stain is turned over, we strongly encourage that you visualize and capture images immediately following the final wash for optimal signal detection.

Answer Id: E12567

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Product FAQ

How can I prevent poor or dim staining when using Alkaline Phosphatase Live Stain?

Answer

Serum or serum replacement components in the growth medium may cause background and this can result in poor or dim staining. After the removal of the growth medium, gently wash the culture with pre-warmed DMEM/F-12 (Cat. No. 10565-018) for 5 minutes. Aspirate and repeat once before adding the AP Live Stain. To further decrease background staining, perform 3 separate washes of 5 minutes each with DMEM/F-12 for a total of 15 minutes following the staining, and visualize immediately.

Answer Id: E12574

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Product FAQ

How do I prepare the StemPRO hESC SFM complete medium?

Answer

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Answer Id: E5304

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Product Literature

Brochure: 3D cell culture product selection guide for organoids and spheroids

Product Literature

Fact Sheet: BenchStable Cell Culture Media green features

Product Literature

Flyer: Mass spec rewards program 2019

Product Literature

Gibco B-27 Plus Neuronal Culture System カタログ

Manual / Product Insert

GIBCO Human Neural Stem Cells (H9 hESC-Derived)

Version: 7 December 2009
Catalog #
  • N7800100(Discontinued)
  • N7800-100-LSG
  • N7800-200-LSG

Product Literature

Cell therapy research product selection guide

Manual / Product Insert

User Guide: B-27 Supplement (50X)

Product Literature

Brochure: Gibco CTS Series (Japanese)

Manual / Product Insert

Advanced Reduced Serum Media

Product Literature

Cell culture product catalog 2016 [JA]

Product Literature

Brochure: B-27 Plus Neuronal Culture System

Manual / Product Insert

Episomal iPSC Reprogramming Vectors FAQs

Version: 15 June 2012
Catalog #

Product Literature

Application Note: Generation of MCF7 spheroids in serum-free conditions

Manual / Product Insert

Rat Glial Precursor Cells (GPCs)

Version: 14 May 2009
Catalog #

Manual / Product Insert

User Guide: Fusion Medium Kit (Prototype)

Version: MAN0016208 Rev. 1.0 (07Oct2016)

Product Literature

BenchStable Cell Culture Media

Manual / Product Insert

Protocol: Generation of hiPSCs from Fibroblasts using Episomal Vectors

Version: MAN0007034 RevA.0 (23 Jan 2013)

Product Literature

Gibco Neurobiology Protocol Handbook

Product Literature

SILAC Application Note 2.1

Product Literature

Brochure: Gibco basal medium chart (Japanese)

Manual / Product Insert

Alkaline Phosphatase Live Stain (500X)

Version: 12 December 2011
Catalog #

Manual / Product Insert

StemPro BG01v/EG Cells

Version: Version B, 30 Sep 2008

Manual / Product Insert

GlutaMAX-I (100X)

Version: MAN0007318 1.00 (12 Jun 13)

Product Literature

CytoTune™ - iPS Reprogramming Kit FAQs