How Gibson Assembly® works Homology requirements Choosing your approach Vector selection Competent cell selection, Building large DNA constructs that contain no extraneous sequences is often a challenging task.
Introduction to type IIS restriction enzymes Golden Gate cloning How does Golden Gate Assembly work? Site directed mutagenesis Plasmid-based reverse genetics systems Conclusion , Discovered more than 50 years ago, restriction enzymes are foundational elements of molecular biology and genetic...
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources., For Research Use Only. Not for use in diagnostic procedures.
Optimize your experiments to get the best results. We’ve compiled a detailed knowledgebase of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, GeneArt Site-Directed Mutagenesis Kits, Need more information?
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Although restriction enzymes are widely used in molecular cloning , their use as molecular tools extends to other common applications in molecular biology. Two important applications are DNA fingerprinting and methylation analysis, which are methods to map sequences and analyze epigenetic patterns...
There are many options available for protein expression from cloned DNA. These include cell-free extracts ( in vitro expression systems), and bacterial, yeast, insect, and mammalian cell systems, each with its own advantages and drawbacks.
Competent cells for everyday bacterial transformation have broad utility for propagating vectors, subcloning DNA fragments, and other routine molecular biology procedures. To accommodate various workflows, the most popular competent cells for subcloning come in several formats including standard,...
When you are trying out a new cloning method or simply looking for a recommendation this section covers common cloning methods and techniques., FastDigest restriction enzymes, CloneJET kits, Ligase Independent Cloning, Alkaline Phosphatases and more.
Cloning methods rely on molecular biological processes that occur in nature. The techniques are continually being refined and simplified; therefore, many strategies nowadays permit cloning of sequences of interest from their sources more efficiently.
If you are already performing cloning and looking for a specific reagent to meet your research needs, this area has everything you need to proceed., Chemically, electrocompetent, expression and competent cells for specialized applications in multiple packaging formats.
Site-directed mutagenesis techniques allow specific introduction of variations at desired locations within DNA, without the need for complete de novo DNA synthesis. Mutagenesis of existing genes can help you find answers for your research, such as: Investigate active sites, structure-function...
Find the answer quickly by clicking your desired question. Q- What if my fragments contain repetitive sequences? Q- Can I clone 5 fragments using the seamless assembly kit, even if it’s not supported by your web tool? Q- What tips can you provide to minimize the appearance of “empty vector” clones?
GeneArt Seamless Cloning and Assembly enables cloning of up to 4 DNA fragments simultaneously into virtually any linearized vector in 30 minutes, without extra DNA sequences, restriction endonucleases or ligation.
Gibson Assembly Cloning is an elegant and robust seamless or scar less cloning methodology that has been widely adopted by the scientific community and enables the assembly of multiple DNA fragments regardless of length or end compatibility in a highly efficient, seamless method.