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Do you offer any kits for forensic DNA purification? Product FAQ

Answer

Yes, we offer our ChargeSwitch Forensic DNA Purification Kit. Please read more about DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/forensic-dna-extraction.html).

Answer Id: E7669

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What can I use to extract DNA from FFPE (formalin fixed paraffin embedded) samples? Product FAQ

Answer

We offer 2 kits: RecoverAll Total Nucleic Acid Isolation Kit for FFPE and MagMAX FFPE DNA/RNA Ultra Kit Read more about the differences between these kits here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/dna-extractions-working-with-ffpe-samples.html).

Answer Id: E7673

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What kit should I use for blood genomic DNA isolation? Product FAQ

Answer

Please see the following web page (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html) for a comparison of kits that can be used to isolate genomic DNA from blood.

Answer Id: E7668

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What are the general genomic DNA requirements for the CytoScan HT-CMA 96F assay? Product FAQ

Answer

The DNA requirements for the Cytoscan HT-CMA assay are outlined below:
a. DNA must be double-stranded genomic DNA.
b. DNA must be free of PCR inhibitors. Examples of DNA polymerase inhibitors include high concentrations of heme (from blood) and high concentrations of chelating agents such as EDTA. The genomic DNA extraction/purification method should render DNA that is generally salt-free because high concentrations of particular salts can also inhibit enzyme reactions
c. DNA must not be contaminated with other human genomic DNA sources or with genomic DNA from other organisms.
d. DNA must not be degraded.
e. DNA should have an A260/A280 between 1.8 - 2.0 (numeric rounding allowed) and A260/A230 greater than 1.5.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Answer Id: E18680

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I'm interested in isolating plant DNA, but am worried about the abundance of carbohydrates and complex polysaccharides in the tissue. What kit do you recommend for this? Product FAQ

Answer

We offer several kits specifically designed to isolate plant DNA depending on the amount of starting material and chemistry you are interested in using. Please use this link (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/plant-dna-extraction.html) for a comparison of our kits available for plant DNA extraction.

Answer Id: E7670

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I want to isolate genomic DNA from bacteria. How do I do this? Product FAQ

Answer

We would recommend using our PureLink gDNA Mini Kit or our ChargeSwitch gDNA Mini Bacteria Kit (Cat. No. CS11301), which can isolate both gram-positive and gram-negative bacteria. No centrifugation or filtration steps are necessary using this simple one-tube protocol. Read more about bacterial DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/bacteria-dna-extraction.html).

Answer Id: E7671

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User Guide: Arcturus Paradise PLUS Reagent System-FFPE Tissue Staining, Extraction, Isolation, Amplification, Microarray Labeling Manual / Product Insert

  • Pub. No.: ebeea603a4638d6d3ad321b2de51ff8b82e4d289
  • Version: 12872-00 Rev. D 07.2010
Catalog #

User Bulletin: MagMAX Cell-Free DNA Isolation Kit - Isolation of cfDNA from small volumes of plasma & serum samples Manual / Product Insert

  • Pub. No.: 0afd80d83a62469d1e7cd0daa15b8c13f4b3385e
  • Version: MAN0015629 Rev.B.0 (10May2016)
Catalog # A29319

What is the recommended genomic DNA extraction method to be used for the CytoScan HT-CMA assay? Product FAQ

Answer

Genomic DNA extraction and purification methods that meet the general DNA requirements for this kit should yield successful results. Methods that include boiling or strong denaturants are not acceptable because the DNA would be rendered single-stranded and can no longer be accurately quantitated using a PicoGreen dye-based assay.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Answer Id: E18681

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What products does Thermo Fisher Scientific sell for plasmid purification? Product FAQ

Answer

Please visit our Plasmid DNA Purification page for the most up-to-date information:
http://www.thermofisher.com/us/en/home/life-science/DNA-RNA-Purification-Analysis/DNA-Purification/Plasmid-DNA-Purification.html

Answer Id: E3112

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User Guide: GMO Extraction Kit - Extraction and purification of DNA from food and feed for next-generation sequencing applications Manual / Product Insert

  • Pub. No.: f22b64e175c653efc973237415a75a4fb8547489
  • Version: C.0
Catalog # 4466336

Product Sheet: PrepFiler Express Forensic DNA Extraction Kit Manual / Product Insert

  • Pub. No.: de120fd08d77d29a6d7341d13ad87e3f7ebd604c
  • Version: E
Catalog # 4441352

What is the time requirment for DNA extraction when using the PicoPure DNA Extraction kit? Product FAQ

Answer

The total incubation time depends on the tissue source for DNA extraction. Non-formalin fixed samples are more efficient, taking approximately 3 hours. Formalin-fixed tissues require more time due to cross-linking, taking approxiately 16 hours or more.

Answer Id: E9796

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Flyer: PrepFiler forensic DNA extraction kits Product Literature

Isolation and purification of genomic DNA and viral RNA from clinical samples Product Literature

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