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Lot # Z04B033 Certificate

Catalog # J67858.AE

Lot # Q24B026 Certificate

Catalog # J67858.AE

Do you offer any kits for forensic DNA purification? Product FAQ

Answer

Yes, we offer our ChargeSwitch Forensic DNA Purification Kit. Please read more about DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/forensic-dna-extraction.html).

Answer Id:: E7669

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Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis. Citations & References

  • Authors: El Maaiden E, El Kharrassi Y, Essamadi AK, Moustaid K, Nasser B,
  • Journal: Methods Protoc
  • PubMed ID: 31164619

What can I use to extract DNA from FFPE (formalin fixed paraffin embedded) samples? Product FAQ

Answer

We offer 2 kits: RecoverAll Total Nucleic Acid Isolation Kit for FFPE and MagMAX FFPE DNA/RNA Ultra Kit Read more about the differences between these kits here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/dna-extractions-working-with-ffpe-samples.html).

Answer Id:: E7673

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What are the general genomic DNA requirements for the CytoScan HT-CMA 96F assay? Product FAQ

Answer

The DNA requirements for the Cytoscan HT-CMA assay are outlined below:
a. DNA must be double-stranded genomic DNA.
b. DNA must be free of PCR inhibitors. Examples of DNA polymerase inhibitors include high concentrations of heme (from blood) and high concentrations of chelating agents such as EDTA. The genomic DNA extraction/purification method should render DNA that is generally salt-free because high concentrations of particular salts can also inhibit enzyme reactions
c. DNA must not be contaminated with other human genomic DNA sources or with genomic DNA from other organisms.
d. DNA must not be degraded.
e. DNA should have an A260/A280 between 1.8 - 2.0 (numeric rounding allowed) and A260/A230 greater than 1.5.

Answer Id:: E18680

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What kit should I use for blood genomic DNA isolation? Product FAQ

Answer

Please see the following web page (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html) for a comparison of kits that can be used to isolate genomic DNA from blood.

Answer Id:: E7668

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I'm interested in isolating plant DNA, but am worried about the abundance of carbohydrates and complex polysaccharides in the tissue. What kit do you recommend for this? Product FAQ

Answer

We offer several kits specifically designed to isolate plant DNA depending on the amount of starting material and chemistry you are interested in using. Please use this link (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/plant-dna-extraction.html) for a comparison of our kits available for plant DNA extraction.

Answer Id:: E7670

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I want to isolate genomic DNA from bacteria. How do I do this? Product FAQ

Answer

We would recommend using our PureLink gDNA Mini Kit or our ChargeSwitch gDNA Mini Bacteria Kit (Cat. No. CS11301), which can isolate both gram-positive and gram-negative bacteria. No centrifugation or filtration steps are necessary using this simple one-tube protocol. Read more about bacterial DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/bacteria-dna-extraction.html).

Answer Id:: E7671

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Extraction and isolation of mRNA from adult articular cartilage. Citations & References

  • Authors: Adams ME; Huang DQ; Yao LY; Sandell LJ
  • Journal: Analytical Biochemistry
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What is the recommended genomic DNA extraction method to be used for the CytoScan HT-CMA assay? Product FAQ

Answer

Genomic DNA extraction and purification methods that meet the general DNA requirements for this kit should yield successful results. Methods that include boiling or strong denaturants are not acceptable because the DNA would be rendered single-stranded and can no longer be accurately quantitated using a PicoGreen dye-based assay.

Answer Id:: E18681

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Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples. Citations & References

  • Authors: Smith K, Diggle MA, Clarke SC
  • Journal: J Clin Microbiol
  • PubMed ID: 12791861
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Developmental Validation of the PrepFiler™ Forensic DNA Extraction Kit for Extraction of Genomic DNA from Biological Samples* Citations & References

  • Authors: Brevnov, MG; Pawar, HS; Mundt, J; Calandro, LM; Furtado, MR; Shewale, JG
  • Journal: JOURNAL OF FORENSIC SCIENCES

What products does Thermo Fisher Scientific sell for plasmid purification? Product FAQ

Answer

Please visit our Plasmid DNA Purification page for the most up-to-date information:
http://www.thermofisher.com/us/en/home/life-science/DNA-RNA-Purification-Analysis/DNA-Purification/Plasmid-DNA-Purification.html

Answer Id:: E3112

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