The DNA requirements for the Cytoscan HT-CMA assay are outlined below:
a. DNA must be double-stranded genomic DNA.
b. DNA must be free of PCR inhibitors. Examples of DNA polymerase inhibitors include high concentrations of heme (from blood) and high concentrations of chelating agents such as EDTA. The genomic DNA extraction/purification method should render DNA that is generally salt-free because high concentrations of particular salts can also inhibit enzyme reactions
c. DNA must not be contaminated with other human genomic DNA sources or with genomic DNA from other organisms.
d. DNA must not be degraded.
e. DNA should have an A260/A280 between 1.8 - 2.0 (numeric rounding allowed) and A260/A230 greater than 1.5.
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Answer Id: E18680
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