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Does AmpliTaq DNA Polymerase have reverse transcriptase activity? Product FAQ

Answer

Yes, AmpliTaq DNA Polymerase has been reported to exhibit reverse transcriptase activity, but it is such a low and inefficient activity that it is neither useful nor harmful to the RNA PCR experiments.

Answer Id: E1071

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Do you offer master mixes containing Bst DNA Polymerase for LAMP or RT-LAMP? Product FAQ

Answer

Yes. Bst DNA Polymerase is available in master mix format together with SuperScript IV Reverse Transcriptase. SuperScript IV RT-LAMP Master Mix (Cat. No. A51801, A51802, A51803) comes in a formulation with glycerol and can be used for amplification from DNA and RNA targets.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Answer Id: E21621

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Is Phi29/EquiPhi29 DNA Polymerase suitable for amplification of short DNA fragments? Product FAQ

Answer

The amplification efficiency of MDA (multiple displacement amplification) reaction rapidly diminishes as the molecular weight of the starting material decreases, thus making the polymerase unsuitable for amplification of low-molecular weight DNA. However, it is suitable for amplification from extremely low amounts of starting DNA material, making it ideal for single cell analysis.

Answer Id: E16592

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What type of DNA template can be amplified by EquiPhi29 DNA Polymerase? Product FAQ

Answer

Both linear and circular DNA templates can be amplified by EquiPhi29 DNA Polymerase.

Answer Id: E16591

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What is the amplification reaction temperature range for EquiPhi29 DNA Polymerase? Product FAQ

Answer

EquiPhi29 DNA Polymerase is a thermostable enzyme. The optimal reaction temperature range is 42-45 degrees C, however it will also amplify DNA at 30 degrees C.

Answer Id: E16594

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T4 DNA Polymerase Manual / Product Insert

  • Version: 07/10/03
Catalog #

How can I avoid non-specific DNA amplification with EquiPhi29 DNA Polymerase? Product FAQ

Answer

EquiPhi29 DNA Polymerase is a thermostable enzyme with a fast reaction speed. Amplification at 45 degrees C for 1-2 hr is sufficient to obtain specific product amplification without background amplification. The reaction is saturated after 3 hr of incubation.

Answer Id: E16593

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What is the difference between Platinum Taq DNA Polymerase and Platinum Taq DNA Polymerase, DNA-free? Product FAQ

Answer

Platinum Taq DNA Polymerase, DNA-free is manufactured using closed and single-use system technology to minimize DNA contamination risk. Platinum Taq DNA Polymerase, DNA-free provides the same high level of performance and lot-to-lot consistency that is expected for the standard Platinum Taq DNA Polymerase.

Answer Id: E17100

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Is EquiPhi29 DNA Polymerase suitable for single cell DNA amplification? Product FAQ

Answer

Yes. The recommended protocol is supplied in the manual (https://www.thermofisher.com/order/catalog/product/A39390). Alternatively, amplification from single cells using EquiPhi29 DNA polymerase can be reviewed in the literature:

Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles.
Authors: Stepanauskas R, Fergusson EA, Brown J, Poulton NJ, Tupper B, Labonté JM, Becraft ED, Brown JM, Pachiadaki MG, Povilaitis T, Thompson BP, Mascena CJ, Bellows WK, Lubys A
Journal: Nat Commun 2017; 8(1):84
PubMed ID: 28729688

Answer Id: E16600

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What enzymes can I use to convert a molecule with a 5' or 3' overhang to a blunt molecule? What is the main difference between them? Product FAQ

Answer

T4 DNA polymerase and Klenow fragment of E.coli DNA polymerase can both convert overhangs to blunt molecules. The 3' - 5' exonuclease activity of the T4 DNA polymerase is much more efficient than Klenow.

Answer Id: E6652

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What are the components of Taq DNA Polymerase buffer and Platinum Taq DNA Polymerase buffer? Product FAQ

Answer

Taq DNA Polymerase buffer and Platinum Taq DNA Polymerase buffer is supplied as a 10X concentrate and should be diluted 1:10 (1 part buffer + 9 parts other components = 10 parts final reaction volume).
Buffer composition (10X): 200 mM Tris-HCl (pH 8.4), 500 mM KCl.
These PCR buffers do not contain magnesium chloride. A separate tube of 50 mM MgCl2 is provided so that users can control the amount in their reactions.

Answer Id: E4053

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DNA Polymerase I (Ecoli) Manual / Product Insert

  • Version: MAN0001320 1.01 (28 Sep 01)
Catalog #

What are the purity requirements for Platinum Taq DNA Polymerase, DNA-free? Product FAQ

Answer

Platinum Taq DNA Polymerase, DNA-free is certified to contain low-DNA contamination level: ≤0.01 copy of bacterial gDNA per enzyme unit; ≤0.001 copy/enzyme unit of human gDNA; ≤0.01 copy/enzyme unit of plasmid DNA.

Answer Id: E17101

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What is the unit concentration of Platinum Taq DNA Polymerase, DNA-free? Product FAQ

Answer

Platinum Taq DNA Polymerase, DNA-free is available in 5.0 U/µL enzyme concentration.

Answer Id: E17107

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Can I use Platinum Taq DNA Polymerase, DNA-free in qPCR? Product FAQ

Answer

Yes, Platinum Taq DNA Polymerase, DNA-free can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.

Answer Id: E17103

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