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DNA labeling by nick-translation Second strand cDNA synthesis DNA blunting by fill-in 5'- overhangs Random-primed DNA labeling Labeling by fill-in 5'- overhangs of dsDNA DNA sequencing Site-specific mutagenesis Second strand cDNA synthesis Random-primed DNA labeling Labeling by fill-in 5'- overhangs...

Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime technology is available in Taq, Pfx , and GC-Rich formulations., The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during...

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase, available in standard and hot-start formats , that offers a balance between performance and value. DreamTaq DNA polymerases are supplied with specially optimized buffers that enable robust DNA amplification with minimal...

Isolated from the thermophilic bacterium Thermus aquaticus , Taq DNA polymerase is one of the best-known thermostable DNA polymerases used in PCR amplification of DNA targets. The native enzyme is purified from Thermus aquaticus YT1 .

Thermo Fisher Scientific has developed a manufacturing process using Single Use System (SUS)-based technology with extensive quality control testing to drastically reduce the risk of DNA contaminates in the PCR reagents for commercial supply.

Applied Biosystems AmpliTaq Gold and AmpliTaq Gold 360 DNA Polymerases are hot-start enzymes which utilize a chemical modification to inhibit enzyme activity at room temperature. Upon activation the modifier is permanently released, resulting in an enzyme that becomes active at a temperature above...

Fusion DNA polymerases are genetically engineered enzymes comprising two constituent elements: These chimeric proteins are powerful enzymes that have improved PCR by enhancing its efficiency and helping enable a variety of new applications. DNA polymerase Double-stranded DNA (dsDNA) binding protein.

High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusionhigh-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme.

Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs.

DNA polymerase is an essential component for PCR due to its key role in synthesizing new DNA strands. Consequently, understanding the characteristics of this enzyme and the subsequent development of advanced DNA polymerases is critical for adapting the power of PCR for a wide range of biological...

We offer an extensive portfolio of Thermo Scientific restriction enzymes and modifying enzymes including DNA and RNA polymerases, phosphatases, kinases, and nucleases. Whatever your downstream applications are, it is critical to use reliable, high-quality, high-purity reagents in your experiments.

Thermo Scientific Phusion Plus DNA Polymerase is the latest addition to the Phusion high-fidelity DNA polymerase family, which has been referenced in thousands of research publications. As a hot-start, proofreading PCR enzyme, Phusion Plus DNA polymerase enables generation of PCR amplicons with very...

Alkaline phosphatase and polynucleotide kinase for DNA and RNA dephosphorylation or phosphorylation., Properties of RNA polymerases for in vitro transcription, RNA labeling, synthesis of antisense RNA and siRNA, studies of RNA splicing and secondary structure.

This calculator generates a pipetting table for setting up PCR reactions with Thermo Scientific Phusion DNA Polymerases. The application will automatically calculate the pipetting volumes required for a premix and the volume of the premix needed for the final reaction.

in vitro Custom functional testing, tailored packaging, and filling to meet your assay requirements and specifications Availability of lyo-ready enzyme formulations without glycerol, for compatibility with lyophilized assays Manufactured in ISO 13485 or ISO 9001 certified facilities–high product...