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Lentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.

Silencer Silencer Silencer, Brummelkamp, TR, Bernards, R, and Agami, R. (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296: 550-3. Sui G, Soohoo C, Affar EB, Gay F, Shi Y, Forrester WC, and Shi Y.

Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments.

The rapidly growing interest in AAV-based gene therapies has led to the need for more cost-effective and scalable AAV vector production platforms. Thermo Fisher Scientific offers innovative products and services that are designed to help accelerate your gene therapy projects.

There are a number of biological, chemical, and physical methods for introducing nucleic acids into cells. Not all of these methods can be applied to all types of cells and experimental applications, and there is a wide variation amongst them with respect to transfection efficiency, cell toxicity,...

Applications like engineering plants for phytoremediation or biofuel research often involve complex design and assembly of DNA elements that require advanced cloning tools. Moreover, if the ultimate goal is expression of foreign genes in plants, there are more extensive technical challenges in plant...

In this article, we share seven must-have tips for your ligation reactions: Consider your cloning strategy Check the ends of your DNA inserts Set up ideal reaction conditions Avoid inhibitors Visualize your ligation reactions on a gel Run controls Check to make sure your ligase is active , Molecular...

Deciding whether you need transient or stable transfection depends on the time frame and ultimate goal of the experiment you wish to conduct. Transiently transfected cells are typically harvested 24–96 hours post-transfection and are often used for studying the effects of short-term expression of...

The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones.

Protein purification withaffinity tags such asglutathione S-transferase(GST), histidine(HIS), and other affinity tags, enables purification of proteinswithboth known andunknown biochemical properties. Therefore, this methodology has becomeawidely used research tool for determiningthebiological...

Vaccine production is complex. Manufacturers face strict regulatory controls during the development process while also anticipating global demand and the eventual production challenges of quantity and quality. Time-to-market and cost effectiveness are constant, critical considerations.

The in vitro synthesis of proteins in cell-free extracts is an important tool for molecular biologists and has a variety of applications, including the rapid identification of gene products (e.g., proteomics), localization of mutations through synthesis of truncated gene products, protein folding...

Successful transfection is influenced by many factors—the choice of the transfection method, health and viability of the cell line, number of passages, degree of confluency, quality and quantity of the nucleic acid used, and the presence or absence of serum in the medium can all play a part in the...

Many researchers now use small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Here we describe five methods for producing siRNAs for use in mammalian RNAi experiments. Each of these methods has its advantages and drawbacks.

In vitro protein expression (also known as in vitro translation, cell-free protein expression, cell-free translation, or cell-free protein synthesis) is a technique that enables researchers to rapidly express and manufacture small amounts of functional proteins.