Product Literature

Flyer: NUMI Promotion Apr-Jun 2019 Singapore

Manual / Product Insert

PureLink™ Viral RNA/DNA Kit

Version: MAN0001750 100008490 (17 Oct 09)

Manual / Product Insert

User Guide: GeneJET RNA Purification Kit

Version: Jan. 2015

Manual / Product Insert

User Guide: GeneJET Plant RNA Purification Kit

Version: Jan. 2015

Product FAQ

Can I purchase any of the buffers that come with the MagMAX FFPE DNA/RNA Ultra Kit separately?

Answer

Protease Digestion Buffer, Binding Solution, and DNA Wash Buffer are also available as standalone Cat. No. A32796. For processing sections that are thicker than 40 µm, additional reagents would be required.

Answer Id: E13122

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Product Literature

Application Note: The human microbiome in 2015—PureLink Microbiome DNA Purification Kit

Product FAQ

I’m getting poor quality/poor purity of RNA after isolation with the RiboPure RNA Purification kit. What could be the cause of this?

Answer

Poor quality/poor purity RNA is typically due to the following:

- Partially degraded RNA: Use fresh tissue or cells, or use RNAlater Stabilization Solution if tissue cannot be frozen immediately.
- DNA contamination: If the sample contains organic solvents, strong buffers, or an alkaline solution, we recommend performing phase separation at 8 degrees C.
- A low A260/A280 ratio (<1.6): This indicates that an insufficient amount of the TRI Reagent was used. Incubation of the homogenate at room temperature for 5 minutes can help nucleoproteins dissociate from RNA. Phenol contamination could also cause a low absorbance ratio.

Answer Id: E7873

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Product FAQ

I am planning on using your MagMAX FFPE DNA/RNA Ultra Kit. I don't want to perform a deparaffinization step using organic solvents, is there an alternative reagent or method I can use?

Answer

Yes, we offer the AutoLys M Tube system, which creates cleared lysates from FFPE tissue samples without the need for deparaffinization or wash steps, while increasing DNA and RNA yields.

Answer Id: E16492

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Product Literature

Rapid DNA purification with the KingFisher Plant DNA Kit from variable plant material

Manual / Product Insert

User Guide: GeneJET FFPE DNA Purification Kit

Version: Jan. 2015

Manual / Product Insert

User Guide: GeneJET Whole Blood Genomic DNA Purification Mini Kit

Version: MAN0012667 Rev. B.0 (12October2016)

Manual / Product Insert

Insert: Tempus™ 12-Port RNA Isolation Reagent Kit

Version: 4382074 REV A 12/2007

Manual / Product Insert

User Guide: Genomic DNA Purification Kit, K0512

Version: Jan. 2015
Catalog #

Product FAQ

Does the DRR (DNase Binding Reagent) from RapidOut DNA Removal Kit bind to other proteins? Is the kit suitable for removal of other proteins from the reaction mixture?

Answer

We have tested the kit for DNase I removal only. Although DRR can bind to other proteins, we cannot provide any recommendations for removal of protein contaminants from the enzymatic mixtures. If the RNA sample contains a considerable amount of protein contaminants, modify the RNA purification protocol to reduce protein contamination.

Answer Id: E8856

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Product Literature

Technical Bulletin: Rapid DNA Purification from Different Plant Samples with the KingFisher Pure DNA Plant Kit

Product Literature

Brochures & Specifications: DNA-free RNA Isolation for RT-PCR: RNAqueous® –4PCR Kit: Product Bulletin

Manual / Product Insert

User Guide: GeneJET Genomic DNA Purification Kit

Version: MAN0012663 Rev. B.0 (12October2016)
Catalog #
  • K0721
  • K0722
  • K0729-LSG
  • R2171-LSG
  • R2181-LSG
  • R2191-LSG
  • R2201-LSG
  • R2211-LSG

Manual / Product Insert

User Guide: PureLink Viral RNA/DNA Mini Kit

Version: MAN0000562 Rev, A,0 (16Jun2016)
Catalog #

Manual / Product Insert

User Guide: GeneJET Viral DNA and RNA Purification Kit

Version: Jan. 2015
Catalog #

Manual / Product Insert

User Guide: PureLink Pro 96 RNA Purification Kit

Version: MAN0001670 Rev. C.0
Catalog #

Manual / Product Insert

User Guide: TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System

Version: MAN0016165 Rev. A.0 (09Nov2016)
Catalog #

Manual / Product Insert

PureLink Micro-to-Midi Total RNA Purification System -Custom Kit for Isis

Version: MAN0000595 A (20 Dec 2006)

Manual / Product Insert

ChargeSwitch Forensic DNA Purification Kits

Version: Version A 01/03/05

Manual / Product Insert

PureLink Pro 96 Viral RNA/DNA Kit

Version: Rev date: 7 May 2009
Catalog #

Manual / Product Insert

User Guide: Trizol Plus RNA Purification Kit

Version: MAN0000561 Rev. A.0 (09Nov2016)
Catalog #

Manual / Product Insert

PureLink Plant Total DNA Purification Kit

Version: 27 September 2012
Catalog #

Manual / Product Insert

User Guide: Viral DNA/RNA Purification Kit (Eng)

Version: May 2015
Catalog #
  • DK0011(Discontinued)

Manual / Product Insert

Protocol: DNA-free™ Kit: DNase Treatment and Removal Reagents (English )

Version: 1906M Rev. E 02.2013
Catalog #
  • AM1906
  • AM1906M(Discontinued)

Manual / Product Insert

User Guide: JetFlex Genomic DNA Purification Kit

Version: MAN0001744 Rev. A.0 (15Apr2016)

Manual / Product Insert

Insert: Tempus™ Spin RNA Isolation Reagent Kit

Version: 4382075 REV B 5/2007

Manual / Product Insert

User Guide: GeneJET Plant Genomic DNA Purification Mini Kit

Version: MAN0016131 Rev. A.0 (12October2016)

Product Literature

Isolation and purification of genomic DNA and viral RNA from clinical samples

Manual / Product Insert

PureLink HQ 96 Plasmid DNA Purification Kit

Version: Version C, 5 May 2006
Catalog #

Manual / Product Insert

User Guide: MagJET Viral DNA and RNA Kit

Version: Jan. 2015
Catalog #
  • K2781(Discontinued)
  • K2782(Discontinued)

Product Literature

Technical Bulletin: DNA Purification from Blood Samples with the KingFisher Pure DNA Blood Kit

Manual / Product Insert

PureLink 96 RNA Purification Kit

Version: Version C 20 April 2005
Catalog #
  • 12173011(Discontinued)

Manual / Product Insert

User Guide: JetQuick Genomic DNA Purification Kit

Version: MAN0001742 Rev. A.0 (15Apr2016)

Manual / Product Insert

PureLink Total RNA Blood Purification Kit

Version: Version B 4 April 2005
Catalog #

Product FAQ

What is different about the MagMAX CORE Nucleic Acid Purification Kit compared to the other MagMAX kits?

Answer

The MagMAX CORE Nucleic Acid Purification Kit offers pre-mixed, room-temperature reagents, which greatly improve product handling and storage. Specifically, the CORE kit decreases processing times by having pre-mixed washes that do not require the addition of alcohol, a single protocol adapted on multiple KingFisher magnetic particle processors, and a complete protocol that addresses 14 unique sample types.

Answer Id: E16164

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Product FAQ

I have a long double-stranded DNA fragment I would like to isolate. What product do you recommend?

Answer

For biotin-labled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin and MyOne C1 magnetic beads. We recommend our Dynabeads KilobaseBINDER Kit, which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Answer Id: E7924

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Product FAQ

Should I treat my RNA sample with DNAse for FlashTag labeling?

Answer

DNAse treatment is optional. It is not necessary for RNA samples that have trace amounts of genomic DNA contamination, but it may be beneficial for RNA samples that are highly contaminated with genomic DNA, to more accurately quantitate the RNA. After treating your RNA with DNAse, it is essential that the DNase be inactivated completely before proceeding with the FlashTag procedure, to prevent degradation of the FlashTag reagent (Vial 5). A variety of RNA purification columns/kits may be used to inactivate the DNase. Inactivation of the DNase by high temperature may not completely inactivate the enzyme.

Answer Id: E14051

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Product FAQ

What are different methods for cleaning up my miRNA sample?

Answer

- Precipitation of miRNA: Follow the instructions for the mirVANA miRNA Isolation Kit or the mirVANA PARIS RNA and Native Protein Purification Kit.
- Double phenol/guanidium (chaotropic reagent) extraction: Disrupt tissue with phenol/guanidine, then extract with phenol, centrifuge, remove the supernatant, and precipitate the RNA.
- Glass-fiber filter: Disrupt the tissue in a chaotropic agent, bind the RNA to a glass-fiber filter, then wash off unbound DNA/protein/salt/nucleotides and elute the RNA.

Answer Id: E7886

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Product FAQ

With the direct lysis approach to DNA/RNA analysis, can I use heparin- or EDTA-treated blood?

Answer

Unforutnately, the Blood-to-CT and MagMAX kits cannot be used with blood from heparin- or EDTA-stabilized tubes. The reactions require the lysis reagent found in Tempus tubes. Also, the heparin- or EDTA-stabilized tubes don't stabilize the RNA profiles, so we recommend using Tempus tubes.

Answer Id: E7112

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Product FAQ

I am getting shorter reads than I used to. What can cause this?

Answer

A change in the read length is usually caused by something that either changes the migration of the DNA or the efficiency of the sequencing reaction. Some of the things that can cause this are:

• Buffer: Buffer may be prepared incorrectly or on the system for too long.

• Polymer: Polymer may have been on the system the system too long, used past the expiration date, frozen/crystallized and thawed, or mixed with water or buffer during capillary fill. There may be a mismatch between polymer type selected in software and what is installed on the system.

• Environment: Instrument may be operating outside environmental specifications in the Site Preparation Guide. You may have poor/improper instrument ventilation, or air flow may be blowing directly on the system.

• DNA quality: RNA/protein contamination may be reducing the sequencing reaction efficiency. If working with PCR product, there may be carryover of primers and/or dNTPs from the PCR reaction, or the sequencing reaction cleanup is incomplete. If using a kit that has beads in it for the purification, beads may be blocking the capillary tip. If using BigDye XTerminator Purification, heating the XTerminator reagent can cause loss of smaller products.

• DNA quantity: There may be too much DNA competing for entry into the capillary with labeled product, or excessive amounts of DNA creating a temporary blockage of the capillary.

For more information on other causes of short reads and how to address each issue, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.

Answer Id: E6283

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Product Literature

Catalog: Reai-time PCR reagents (Japanese)

Manual / Product Insert

User Guide: Quantifiler Duo DNA Quantification Kit

Version: F
Catalog #

Product Literature

Brochure: QuantiGene FFPE - Formalin Fixed, Paraffin-Embedded Tissue Reagent System

Product Literature

Thermo Scientific Avidin-Biotin Technical Handbook

Manual / Product Insert

User Guide: DreamTaq DNA Polymerase, 5x500U

Version: Jan. 2015
Catalog #

Manual / Product Insert

BLOCK-iT RNAi Basic Control Kit (Human)

Version: Version B 01/13/06
Catalog #
  • K491100(Discontinued)

Manual / Product Insert

RiboPure™-Yeast Kit Manual

Version: 1926M Revision E 2011
Catalog #

Product Literature

Brochure: Pierce Reagents for Quantitative Proteomics: Protein Quantitation Using Mass Spectrometry