Manual / Product Insert

PureLink Pro 96 Viral RNA/DNA Kit

Version: Rev date: 7 May 2009
Catalog #

Manual / Product Insert

PureLink 96 RNA Purification Kit

Version: Version C 20 April 2005
Catalog #
  • 12173011(Discontinued)

Product Literature

Application Note: Comparison of DNA and RNA from fresh-frozen vs. FFPE tissue samples

Manual / Product Insert

User Guide: GeneJET Genomic DNA Purification Kit

Version: MAN0012663 Rev. B.0 (12October2016)
Catalog #
  • K0721
  • K0722
  • K0729-LSG
  • R2171-LSG
  • R2181-LSG
  • R2191-LSG
  • R2201-LSG
  • R2211-LSG

Product FAQ

I see insoluble material in my tube after homogenization of my sample for RNA extraction with TRIzol Reagent. What should I do?

Answer

There are two methods to remove insoluble material:

1. For RNA isolation only: If a lot of insoluble material exists after homogenization and a 5 minute room temperature incubation, remove it by centrifugation at 12,000 x g for 10 minutes at 4 degrees C before adding chloroform (a clear supernatant and jelly-like pellet should be seen). Remove the supernatant and proceed to the next step. Note: This should not be done if subsequent DNA isolation is planned.
2. RNA and DNA isolation: If a lot of insoluble material exists after homogenization and a 5 minute room temperature incubation, the homogenate can be passed through a polypropylene mesh to remove insoluble material that may interfere with precipitation of DNA.

Answer Id: E7862

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Manual / Product Insert

User Guide: Genomic DNA Purification Kit, K0512

Version: Jan. 2015
Catalog #

Product FAQ

Does the DRR (DNase Binding Reagent) from RapidOut DNA Removal Kit bind to other proteins? Is the kit suitable for removal of other proteins from the reaction mixture?

Answer

We have tested the kit for DNase I removal only. Although DRR can bind to other proteins, we cannot provide any recommendations for removal of protein contaminants from the enzymatic mixtures. If the RNA sample contains a considerable amount of protein contaminants, modify the RNA purification protocol to reduce protein contamination.

Answer Id: E8856

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Product Literature

White paper: Single-use technology in production of DNA-free PCR reagents

Product FAQ

I’m getting low yield of RNA/degraded RNA when isolating it extraction with TRIzol Reagent. What could be the causes for this?

Answer

Please review the following causes for low yield of RNA/degraded RNA:

- The RNA may have been concentrated with a SpeedVac system or lyophilized after the last ethanol precipitation. RNA that has been dried completely has decreased solubility. Additionally, if excess centrifugation speeds (higher than 12,000 x g) were used, it is harder to solubilize RNA/DNA.
- The RNA pellet may not be completely solubilized. To increase the rate of solubilization, pipette repeatedly in SDS solution or DEPC-treated water, then heat to 50-60 degrees C. The sample may also have been rich in polysaccharides or proteoglycans. If so, the isopropanol precipitation step should be done with 0.25 volumes of isopropanol and 0.25 volumes of a high salt solution.
- Cells were washed prior to the addition of TRIzol Reagent. Washing cells before the addition of TRIzol Reagent increases the possibility of mRNA degradation.
- The sample was not fully homogenized.
- The tissue was not IMMEDIATELY processed or frozen after removal from the animal or other source.
- The tissue was not completely disrupted; if a centrifugation is done prior to adding chloroform, there should be a white mucus-like pellet. If there is a tan-colored precipitate, this is indicative that not all of the cells have been lysed.
- If a mortar and pestle was used to powder the tissue, RNA and DNA may have stuck nonspecifically to the mortar and pestle. It may be better to use a glass homogenizer and Teflon pestle; add TRIzol Reagent to the homogenizer, then add frozen tissue and homogenize.
- RNA may have been stored after isolation at -20 degrees C instead of -70 degrees C.
- Tissue culture cells were disrupted by trypsin.
- Homogenizing for too long and too continuously in a small volume (e.g., 1 mL) may cause heating of the sample; this may result in degradation of the RNA in the tissue. Samples should be cooled during homogenization, and homogenization should be done in on-off cycles (as opposed to continuously).
- The OD reading may vary due to the solution the sample is stored in AND what it was diluted in prior to quantitation. This can lead to apparently low yields.
- Excess RNAlater Stabilization Solution (>0.05 mL) will reduce RNA recovery and cause problems with phase separation.

Answer Id: E7863

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Product FAQ

Can I purchase any of the buffers that come with the MagMAX FFPE DNA/RNA Ultra Kit separately?

Answer

Protease Digestion Buffer, Binding Solution, and DNA Wash Buffer are also available as standalone Cat. No. A32796. For processing sections that are thicker than 40 µm, additional reagents would be required.

Answer Id: E13122

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Product Literature

Brochure: RNA isolation and purification (non-North America)

Manual / Product Insert

Protocol: DNA-free™ Kit: DNase Treatment and Removal Reagents (English )

Version: 1906M Rev. E 02.2013
Catalog #
  • AM1906
  • AM1906M(Discontinued)

Manual / Product Insert

Manual: User Manual: DNA/RNA Synthesizers Models 392 and 394 (English )

Version: 23 Jun 2011

Product Literature

Flyer: Accelerate gene expression without RNA purification