For Invitrogen BODIPY 493/503 dye (Cat. No. D3922), one may make a stock solution of 1 mg/mL (3.8 mM) in either absolute ethanol or anhydrous DMSO. After resolubilizing in either solvent, store the stock solution frozen, desiccated, and protected from light. The gradual accumulation of moisture in the solvent will promote aggregation over time, hence store the stock solution desiccated.
For long-term storage, dissolve the dye in absolute ethanol, make smaller aliquots in separate vials, and then evaporate off the ethanol using a vacuum pump. Store the solid dye frozen and protected from light.
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Answer Id: E10393
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