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StemPro® Accutase® Cell Dissociation Reagent Manual / Product Insert

  • Pub. No.: 548eff9dee2eb6c6150018e33064760921998bb3
  • Version: MAN0007245 1.00 (14 May 14)
Catalog # A1110501

StemPro® Accutase® Cell Dissociation Reagent Brochure Product Literature

With StemPro Accutase Cell Dissociation Reagent, do I need to stop the dissociation reaction with serum? Product FAQ

Answer

No. StemPro Accutase is gentle enough that only dilution of the reagent with DPBS or media is required to stop the dissociation activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18602

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Do I need to dilute StemPro Accutase Cell Dissociation Reagent before using it? Product FAQ

Answer

No. StemPro Accutase is supplied as a convenient, ready to use reagent. No dilution is required prior to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18603

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Do I need to worry about over-dissociating my cells with StemPro Accutase Cell Dissociation Reagent? Product FAQ

Answer

No. However, although StemPro Accutase is gentle on cells, the optimal time for dissociation should be empirically determined for your specific cell type and application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18604

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My StemPro Accutase Cell Dissociation Reagent arrived completely thawed. Can I still use it? Product FAQ

Answer

Yes, as long as the StemPro Accutase is still cool to the touch, it should be okay to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18599

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What are the target cell lines that StemPro Accutase Cell Dissociation Reagent can be used for? Product FAQ

Answer

We recommend using StemPro Accutase wherever gentle and efficient dissociation is needed. We have also validated StemPro Accutase for use with hESC grown in StemPro hESC SFM and for NSC grown in StemPro NSC SFM. All the tests resulted in great recovery of cells without losing their properties and while maintaining good viability. In addition, the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/5029_A1110501_Accutase.pdf) lists examples of cell lines where the application has been validated and published.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18595

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How long can I store StemPro Accutase Cell Dissociation Reagent at 2 to 8 degrees C? Product FAQ

Answer

Once thawed, we recommend using StemPro Accutase within 2 months. However, internal stability data suggests that the enzyme retains activity even after 1 year.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18601

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Can I use StemPro Accutase Cell Dissociation Reagent to dissociate non-adherent cells? Product FAQ

Answer

Yes. StemPro Accutase has been tested to dissociate spheres of neural progenitors (neurospheres).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18596

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I accidentally thawed the StemPro Accutase Cell Dissociation Reagent at 37 degrees C. Can I still use it? Product FAQ

Answer

Yes. If StemPro Accutase is exposed to 37 degrees C just until complete thawing is achieved, it still can be used. However, this could result in decreased enzyme activity and it would take more time to get full dissociation. If you observe this, consider starting with a new bottle. Please note that if a bottle of StemPro Accutase is kept at 37 degrees C for more than one hour, it will lose its activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18600

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My StemPro Accutase Cell Dissociation Reagent arrived partially thawed, can I still use it? Product FAQ

Answer

Yes. We recommend thawing it at room temperature or overnight at 4 degrees C to get complete thawing. Otherwise, place in the freezer and refreeze.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18598

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The frozen bottle of StemPro Accutase Cell Dissociation Reagent shows uneven color distribution and upon thawing, it has layered color distribution. Is this appearance normal? Product FAQ

Answer

Yes. During the freezing procedure or shipping, uneven color can be observed. However, this will not compromise the activity or performance of the enzyme. Just make sure to mix the solution by inverting the tube or bottle to get an even color before use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18597

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What dissociation reagent do you recommend for passaging tumoroids? Product FAQ

Answer

We recommend using StemPro Accutase Cell Dissociation Reagent (Cat. No. A1110501) for passaging tumoroids.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21761

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Which dissociation reagent should I use with the Gibco PSC Definitive Endoderm Induction Kit? Product FAQ

Answer

We recommend using EDTA for passaging PSCs. For seeding cells for definitive endoderm induction, we recommend using StemPro Accutase Cell Dissociation Reagent for small clumps and Gibco TrypLE Enzyme for singularized cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E12479

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What other dissociation reagents have been tested with Gibco Essential 8 Flex Medium? Product FAQ

Answer

Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E10277

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