Manual / Product Insert

E-PAGE MagicMark Unstained Protein Standard

Version: 17 January 2012
Catalog #
  • LC5701(Discontinued)

Product FAQ

What are the apparent molecular weights of the proteins in the E-PAGE SeeBlue Prestained Standard?

Answer

The E-PAGE SeeBlue Prestained Standard is designed for use with E-PAGE pre-cast gels, to visualize protein molecular weight ranges during electrophoresis and evaluate western transfer efficiency. The apparent molecular weights of the proteins in the E-PAGE SeeBlue Prestained Standard are shown below:

- Myosin: 261 kDa in 6% E-PAGE gel
- Phosphorylase B: 173 kDa in 6% E-PAGE gel
- BSA: 97 kDa in 6% E-PAGE gel
- Alcohol dehydrogenase: 42 kDa in 6% E-PAGE gel
- Myoglobin: 21 kDa in 6% E-PAGE gel

Answer Id: E11729

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Product FAQ

Which protein standards do you recommend using with E-PAGE 48 and E-PAGE 96 gels for electrophoresis, Western blotting and fluorescent detection?

Answer

Electrophoresis:
For E-PAGE 48 8% gels, use SeeBlue Plus 2 Pre-Stained Standard, Cat. No. LC5925.
For E-PAGE 96 6% gels, use E-PAGE SeeBlue Pre-Stained Standard, Cat. No. LC5700.

Western Blotting:
For E-PAGE 48 8% gels, use MagicMark XP Western Protein Standard, Cat. No. LC5602.
For E-PAGE 96 6% gels, use E-PAGE MagicMark Unstained Protein Standard, Cat. No. LC5701.

Fluorescence detection:
For E-PAGE 48 8% gels, use BenchMark Fluorescent Protein Standard, Cat. No. LC5928.
For E-PAGE 96 6% gels, use BenchMark Fluorescent Protein Standard, Cat. No. LC5928.

Answer Id: E10771

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Product Literature

MagicMark™ XP Western Protein Standard

Product Literature

Protein Standards Selection Guide

Product FAQ

What are the storage conditions and shelf life for the E-PAGE SeeBlue Prestained Standard?

Answer

We recommend storing the E-PAGE SeeBlue Prestained at 4 degrees C and it is stable for 4 months when properly stored.

Answer Id: E11728

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Manual / Product Insert

E-PAGE SeeBlue Pre-Stained Standard

Version: 17 January 2012
Catalog #

Manual / Product Insert

E-PAGE 48 Protein Electrophoresis System

Version: Rev. A (29 October 2014)
Catalog #

Manual / Product Insert

E-PAGE 96 Protein Electrophoresis System

Version: Ver C, 1/27/05

Product FAQ

What protein markers should be used for E-PAGE gels?

Answer

The following protein MW standards are recommended for E-PAGE 96 gels.

Cat. No. LC5700 E-PAGE SeeBlue Pre-Stained Standard for general electrophoresis
Cat. No. LC5701 E-PAGE MagicMark Unstained Protein Standard for Western blotting
Cat. No. LC5928 BenchMark Fluorescent Protein Standard for fluorescent detection

The following protein MW standards are recommended for E-PAGE 48 gels.

Cat. No. LC5925 SeeBlue Plus 2 Pre-Stained Standard for general electrophoresis
Cat. No. LC5602 MagicMark XP Western Protein Standard for Western blotting
Cat. No. LC5928 BenchMark Fluorescent Protein Standard for fluorescent detection

Answer Id: E4857

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Product FAQ

Can I stain E-PAGE gels with InVision His-Tag In-Gel Stain?

Answer

E-PAGE gels are thicker than standard mini-gels and result in too much background when stained with InVision His-Tag In-Gel Stain. To obtain better staining sensitivity, we recommend transferring proteins of E-PAGE gels onto a nitrocellulose membrane and then staining the blot with the InVision His-tag In-gel Stain as described on page 14 in the manual.

Answer Id: E11179

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Product FAQ

What is special about E-PAGE gels?

Answer

E-PAGE gels are self-contained, pre-cast gels (neutral pH) that include a buffered gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. They are designed for fast, buffer-less, high-throughput protein electrophoresis in a horizontal format and are available in 96-well, 6% and 48-well, 8% formats. Each E-PAGE 96 6% gel contains 96 sample lanes and 8 marker lanes in a patented staggered well-format that is compatible with the standard 96-well plate format for loading with a multichannel pipette or with an automated liquid handling system. Each E-PAGE 48 8% gel contains 48 sample wells and 4 marker wells. The wells are compatible for loading with a multichannel pipette in alternating lanes or with an automated liquid handling system.

Answer Id: E10757

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Product FAQ

How do you recommend staining E-PAGE gels?

Answer

E-PAGE gels are compatible with many standard Coomassie staining, silver staining, or fluorescent staining protocols. E-PAGE gels are thicker than most SDS-PAGE mini-gels, so additional time may be required for staining and destaining steps. We recommend the following stains for E-PAGE Gels. Detailed staining protocols are described on Pages 40-49 of the E-PAGE Technical guide (http://tools.thermofisher.com/content/sfs/manuals/epagetechguide_man.pdf).

Total protein stains:
*SYPRO Ruby Protein Gel Stain (Page 40)
*Coomassie Stain (Page 42)
*SimplyBlue SafeStain (Page 44)
*SilverQuest Silver Staining Kit (Page 47)
*SilverXpress Silver Staining Kit (Page 48)

Specific protein stains:
*Lumio Green Detection Reagent for detecting Lumio fusion proteins Ppage 40)
*InVision His-tag In-Gel Stain for detecting 6X His-tagged proteins (blotting recommended first, Page 49)

Answer Id: E10775

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Product FAQ

What types of membranes are available in the iBlot Transfer Stacks?

Answer

iBlot Transfer Stacks are available with either an integrated 0.2 µm nitrocellulose membrane or a 0.2 µm PVDF membrane. Both types are available in two sizes: a mini size which can accommodate one mini gel (8 x 8 cm), or regular size which can accommodate one E-PAGE gel, one midi gel (8 x 13 cm), or two mini gels (8 x 8 cm).

The catalog numbers are as follows:
IB3010-01 iBlot Gel Transfer Stacks, Regular (10 Blots)
IB3010-02 iBlot Gel Transfer Stacks, Mini (10 Blots)
IB4010-01 iBlot Transfer Stack PVDF, Regular (10 Blots)
IB4010-02 iBlot Transfer Stack PVDF, Mini (10 Blots)

If you would like to blot proteins onto your own membrane rather than the supplied nitrocellulose or PVDF, you can replace the integrated membrane with your desired membrane as described below:

1) Wet the desired blotting membrane in deionized water. (For PVDF membranes, first wet the PVDF membrane in 100% methanol and then rinse in deionized water).
2) Carefully remove the nitrocellulose membrane from the bottom stack using forceps.
3) Place the wetted blotting membrane on the bottom transfer stack. (Membrane should be completely wet, but not dripping; make sure there is not too much excess water.)
4) Be sure to align the membrane flush to the bottom stack gel, and remove any air bubbles using the blotting roller. Proceed with the standard iBlot transfer protocol.

Answer Id: E5027

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Manual / Product Insert

TC-FlAsH Expression Analysis Detection Kits

Version: 06-29-2007
Catalog #
  • A10067(Discontinued)
  • A10068(Discontinued)