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EGF Polyclonal Antibody (Invitrogen™)

EGF Polyclonal Antibody for Western Blot, IHC (P)

EGF Polyclonal Antibody (Invitrogen™)

EGF Polyclonal Antibody for Western Blot

EGF Polyclonal Antibody (Invitrogen™)

EGF Polyclonal Antibody for Western Blot, ELISA

EGF Monoclonal Antibody (OTI2F9), TrueMAB™ (OriGene)

EGF Monoclonal Antibody for IHC (P)

EGF Human ELISA Kit (Invitrogen™)

EGF Human ELISA Kit for ELISA

EGF Recombinant Human Protein

Epidermal growth factor (EGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) retaining the initial Met at the N-terminus.

N-terminal Sequence Analysis: Met 1

Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.) . The EC50 for this effect is typically 20-100 pg/mL.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Background
Human epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serves as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved 6 cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing 9 EGF units, and is proteolyticallycleaved to generate the 53-amino acid peptide hormone.

You may also be interested in the following EGF products:
Kinases
Primary Antibodies
Bioconjugates

EGF Monoclonal Antibody (1H11) (Invitrogen™)

EGF Monoclonal Antibody for Western Blot, IF, ICC, ELISA

pHrodo™ Green Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Green is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Green dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 509/533 nm
• Excellent photostability
• Appropriate for multiplexing with red and deep red fluorophores such as RFP, Alexa Fluor 594, MitoTracker Red, and MitoTracker Deep Red

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

EGF Monoclonal Antibody (3A8) (Invitrogen™)

EGF Monoclonal Antibody for Western Blot, IF, ICC, ELISA

EGF Monoclonal Antibody (9A1) (Invitrogen™)

EGF Monoclonal Antibody for Western Blot, IF, ICC, ELISA

EGF Recombinant Rabbit Monoclonal Antibody (3H11L6) (Invitrogen™)

EGF Recombinant Monoclonal Antibody for ICC, ELISA

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

EGF Human Instant ELISA™ Kit (Invitrogen™)

EGF Human Instant ELISA™ Kit for ELISA

EGF Antibody (3A8) (Thermo Scientific™)

The M806S anti-EGF antibody (Clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11). Typical dilutions for sandwich ELISA include 1-3 µg/ml for coating and 0.125-0.5 µg/ml for detection.

The protein encoded by EGF gene is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds

EGF Recombinant Human Protein, Animal-Origin Free (Gibco™)

Recombinant Human Epidermal Growth Factor (EGF) is a bioactive protein intended for use in cell culture applications. EGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High purity recombinant bioactive protein
Recombinant Human Epidermal Growth Factor is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High biological activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Epidermal Growth Factor (EGF) specific activity is determined by the dose dependent proliferation of mouse BALB/3T3 cells.

Proven compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.