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Find HRP and other enzyme substrates for ELISA applications in a range of sensitivities for different methods including colorimetric, chemiluminescent, and fluorescent detection. Search ELISA buffers Search ELISA plates ELISA builder tool, On this page: Colorimetric substrates Chemiluminescent...

Our specialized products, innovative technologies and customized workflow solutions help expand and compliment the portfolios of life science tool providers., PCR & RT-PCR enzymes DNA/RNA modifying enzymes Restriction enzymes Molecular cloning enzymes Lyo-ready enzymes Enzyme engineering and...

Select from a wide variety of easy-to-use and reliable blocking buffers and reagents for ELISA applications. ELISA buffers and reagents are important components to develop the best assay performance for high sensitivity, low background, and blocking non-specific binding.

Gibco brand media provide the broadest selection of specialty products that are optimized for neural stem cell populations, many of which are free of animal derived components. These media are designed to minimize adaptation time, maximize cell performance, and meet regulatory requirements.

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.

Introduction Plates and coating options Blocking buffers Washing the plate Antibodies Target antigen Enzyme conjugate Substrates and signal detection, Find ELISA Kits by Target Uncoated ELISA Plates Coated ELISA Plates Enzyme Substrates for ELISA Blocking Buffers for ELISA ELISA Instruments and...

Common ELISA formats., Introduction to enzyme probes Common enzyme probe reporters and chromogenic substrates Enzyme reporters for probes Types of substrates Enzyme labeling, Chemiluminescent Western Blot Detection Enzyme Substrates for ELISA Luciferase Assays, Enzymes are used to detect targets of...

Figure 1. Generalized scheme of a typical sandwich ELISA protocol., ELISA development ELISA optimization, Find ELISA Kits by Target ELISA Reagents and Buffers ELISA Enzyme Substrate Selection Guide ELISA Instruments and Equipment , There are many things to consider when developing an ELISA.

For Research Use Only. Not for use in diagnostic procedures., View all Should I run any internal controls with your ELISA kits? Where can I get internal controls? What’s the difference between the chromogen blank and the zero standard in your ELISA kits?

Horseradish peroxidase (HRP)-linked secondary antibodies are commonly used in applications, such as western blotting, ELISA, immuno-histochemistry, and others. For each application, HRP-linked secondary antibodies need substrates that can deliver high signal-to-noise ratios for the chosen detection...

The enzyme-linked immunosorbent assay (ELISA) technique is the gold standard for quantitation of soluble proteins and provides rapid and consistent results that are easy to analyze. Browse our comprehensive and convenient portfolio of nearly 2,000 ELISA products, ranging from ready-to- use coated...

Advancements in mesenchymal stem cell (MSC) research are shedding light on how these stem cells may someday be used in various clinical applications, including immunomodulatory therapies and cell replacement therapies for mesenchymal tissues such as bone and cartilage [1,2].

Optimize your protein and enzyme activity assay experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your...

Precast gels, running buffers, molecular weight markers, and gel stain for sample preparation, loading, and analysis when studying proteins by electrophoresis (SDS-PAGE), Kits, reagents, and devices, including iBlot 2 dry blotting system, SuperSignal chemiluminescent substrates, and iBind system for...

Thermo Fisher Scientific is a global leader in the manufacture and supply of nucleic acid modifying enzymes and polymerases. We offer a combination of proprietary as well as generic off-the-shelf enzymes, and can also custom-manufacture on your behalf.