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EVOS™ XL Core Imaging System

The EVOS XL Core Imaging System is a digital, transmitted light, inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its color camera and high-quality optical system deliver high-definition images with exceptional ease. With a small footprint and an ergonomic design, the all-in-one EVOS XL system is ideal for basic imaging needs and a perfect addition to any cell culture room or facility.

The EVOS XL Core Imaging System offers you these important advantages:

• Easy installation; no maintenance, assembly, alignment, or calibration
• Configured for four objectives
• Fits and operates inside a cell culture hood
• All-in-one design: digital camera, precision optics, LCD display, and USB storage

Imaging Anywhere
The EVOS XL Core system is an integrated transmitted-light inverted imaging system that combines high-quality optics, a 12.1" high-resolution LCD display, and a digital color camera. Images are seamlessly acquired through the user interface using a mouse and the integrated software that includes a variety of features, such as color temperature control. All images can be saved onto the USB device in JPEG, BMP, or TIFF formats. Applications suited for the EVOS XL Core system include basic imaging of live and fixed cells, tissue culture determinations (confluence, density, and growth), stem cell isolation, and analysis of stained tissue slices.

The EVOS imaging systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy it is to operate and amazed at how extraordinary your images look on-screen.

Compare cell imaging systems >

Versatile
The four-position objective turret can be configured to best meet your needs using our full range of long working distance (LWD) and coverslip-corrected objectives. LWD objectives are available from 4x to 40x, and our 2x objective allows viewing of the entire well of a 96-well plate. The compact footprint makes it easy to use the EVOS XL Core system wherever needed; the entire system can be easily moved into a cell culture hood or glove box. The EVOS XL Core system is designed with a universal stage that provides maximum flexibility for slides, culture dishes, and flasks. The optional mechanical stage affords more precise handling and positioning of multiwell plate vessels.

Easy to Use and Reliable
The EVOS XL Core Imaging System is plug-and-play. In addition to an exceptionally easy set-up and installation, it requires no warm-up or cool-down periods. The LED light source offers exceptional stability and durability—turn the unit on and off whenever you need to image a sample. The LED bulbs are rated for >50,000 hours (~17 years) of life, compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long life and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources. Finally, the advanced ergonomic design of the EVOS systems removes the strain associated with conventional microscopes, enables shared viewing, and makes moving the units easy.

Learn more about the EVOS XL Core Imaging System
Explore the entire EVOS line of imaging systems and accessories

System Highlights
Optics: infinity-corrected optical system, RMS-threaded objectives with 45 mm parfocal distance
Objectives (not included): wide selection of high-quality LWD and coverslip-corrected objectives
Illumination: adjustable intensity LED (>50,000-hour life per light cube)
Contrast methods: transmitted light (brightfield and phase contrast)
Objective turret: 4-position, manual control
Condenser: 3-position turret for brightfield and phase contrast
Condenser working distance: 60 mm
Stage: fixed stage
Focus mechanism: coaxial focus knobs with tension control
Course focus: 38 mm/rev
Fine focus: 0.2 mm/rev, precision 0.002 mm
LCD display: 12.1" high-resolution (1024 x 768 pixels) color monitor with adjustable tilt
Camera: Color, 1/2" 2048 x 1536 pixels, 3.1 Megapixels
Image acquisition: embedded operating system with software for image capture and saving via mouse or front-mounted manual buttons
Captured images: color camera; 24-bit full color; TIFF, PNG, jpeg, bmp; 2048 x 1536 pixels
Output ports: 2 USB 2.0 ports
Power supply: AC adaptor; input 100-240 V, 47-63 Hz; output 12 VDC/2.0 A 24 W max
Dimensions: height = 53.3 cm (21.0 in); depth = 40.6 cm (6.0 in); width = 31.8 cm (12.5 in)
Weight: 9.1 kg (20.1 lbs)

FLoid™ Cell Imaging Station

The FLoid® Cell Imaging Station is an affordable, user-friendly imaging solution for the quick detection and verification of fluorescently-labeled samples. The FLoid® Cell Imaging Station captures transmitted light and three-color fluorescent images of your cells and samples right at your benchtop. You do not need to be specially trained or have dedicated staff to operate it. An uncomplicated user interface, streamlined image acquisition process, and real-time, multicolor display allow even imaging novices to produce high-quality images with a few mouse clicks.

The FLoid® Cell Imaging Station offers you these important advantages:

Simplicity—Fully integrated system with intuitive, multilingual user interface
Speed—Get results in a snap, without warm-up, cool-down, or filter changes
Convenience—Capture and print images on your bench, rather than in the darkroom
Information—Find out about the most popular Molecular Probes® reagents and protocols via the user interface
Robustness—No moving parts and long life LEDs for reliable day to day use

Compare cell imaging systems >

High Quality Imaging Anywhere in Your Lab
The FLoid® Cell Imaging Station is a fully integrated devise seamlessly combining high-quality optics, detection in three fluorescence channels as well as transmitted light, a widescreen LCD monitor, camera, image acquisition/processing software, and reagent information and protocols. It features a fixed, high-quality 20x plan fluorite objective, with additional magnification obtainable using the digital zoom. Fluorescence is captured in the three most commonly used channels (DAPI, FITC, and Texas Red filters). Transmitted light images automatically show cells in excellent contrast through a relief phase design, without loss of light. With its innovative light shield, ambient light is blocked so the FLoid® Cell Imaging Station can be placed wherever you need it most - in the tissue culture room, among other cell-based assay instruments, or on your lab bench. FLoid® Cell Imaging Station lets you bring imaging out of the darkroom.

Versatile and Easy to Use
The FLoid® Cell Imaging Station uses an open-stage design providing maximum vessel flexibility for slides, multi-well culture dishes, or even T75 flasks. A focus assist scale makes it easy to find the focus plane. Once samples are in focus, you can use the mouse to change light intensity and switch colors; there is also a scale bar that changes as the digital zoom level is changed, making it easy to estimate and compare sample sizes. The absolute magnification span is from 460X up to 1,840X using the digital zoom. Images can be printed directly using the optional printer, or saved for viewing in one of the four formats (JPEG, TIFF, BMP, or PNG) at a later time or with other software. With printer you can generate sticker-backed prints for immediate notebook mounting. The FLoid® Printer is not included with the FLoid® Cell Imaging Station and must be purchased separately.

Fast and Reliable
The FLoid® Cell Imaging Station requires no warm-up or cool-down periods. It uses LED light sources possessing exceptional stability and durability--so you can turn the FLoid® Cell Imaging Station on and off whenever you produce an image. Because there are no user-changeable parts, the system is exceptionally robust and no components will have "walked away" when you need them. By taking on everyday imaging tasks, the reliable FLoid® Cell Imaging Station helps you protect your investment in other expensive fluorescence equipment.

An Everyday-Use Imager for Every User
Now anyone can quickly start acquiring high-quality fluorescence images. To facilitate straightforward, intuitive operation, the FLoid® Cell Imaging Station features an icon-based user interface with easy-to-use image capture and processing screens, thumbnails of collected images, and quick reference icons. You can set all interface instructions, including protocols, to one of the six built in languages--English, Spanish, German, French, Italian, Japanese, and Chinese.

FLoid® Cell Imaging Station Places Information at your Fingertips
By incorporating the Molecular Probes® reagent selection guide, the FLoid® Cell Imaging Station simplifies fluorescent dye selection and cell staining. You can easily access detailed information on over 160 reagents in 20 application areas directly from the user interface. Each reagent has a quick reference protocol with icons and concise text for ease of use. These reagents have been validated for spectral compatibility with FLoid®, so you can obtain optimal images.

System specifications include:
Objective: Fixed 20X plan fluorite; numerical aperture (NA) = 0.45; working distance (WD) = 5.9 mm.
Color Channels: 4 channels (relief phase, blue, green, and red fluorescence)
Illumination: LED (50,000 hour life), adjustable intensity
Excitation: Blue channel: 390/40 nm; Green channel: 482/18 nm; Red channel: 586/15 nm
Emission: Blue channel: 446/33 nm; Green channel: 532/59 nm; Red channel: 646/68 nm
Captured Images: 16-bit monochrome, TIFF, PNG, JPG, or BMP (1296 × 964 pixels)
LCD display: 15-inch color; display resolution 1366 x 768 pixels; image resolution 1296 x 964 pixels; adjustable tilt
Dimensions: (W x H x D): 15.9" x 21.1 x 13.9" / 40.4 cm x 53.6 cm x 35.3 cm; 28 lbs / 12.7 kg

EVOS™ Light Cube, DAPI

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The DAPI light cube is ideal for use with Acridine, Alexa Fluor 350, DAPI, Hoechst, NucBlue, MarinaBlue, Pacific Blue and other fluorophores with excitation and emission maxima near 357 and 447 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 10X Objective, achromat, LWD, phase-contrast

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.25
• Working Distance: 6.9 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Vessel Holder, two 25 x 75 mm slides

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 25 x 75 mm slides. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type C (Europe)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type C power plugs. The Type C electrical plug (Europlug) is used throughout continental Europe, parts of the Middle East, and much of Africa, South America, Russia, and the former Soviet republics.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type I (Australia)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type I power plugs. The Type I electrical plug is mainly used in Australia and New Zealand, but is also used in China and parts of Asia, South America, and Africa.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type L (Italy)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type L power plugs. The Type L plug is used in Italy, Chile, Uruguay, and parts of North Africa.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Vessel Holder, two 60-mm Petri dishes

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 60 mm Petri dishes. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ XL Core Configured Microscope (Invitrogen™)

The EVOS XL Core Imaging System is a digital transmitted-light inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its simple user interface drives a color camera and high-quality optical system that delivers high-definition images with exceptional ease. Equipped with 4X, 10X, and 20X long working distance (LWD) phase contrast (PH) objectives, the all-in-one EVOS XL Core system is an ideal microscope for basic imaging needs. With a small footprint, an articulated monitor, and ergonomic design, it is a perfect addition to any cell culture room or facility.

The EVOS XL Core Imaging System offers these important advantages:
• Easy installation; no maintenance, assembly, alignment, or calibration
• 4X, 10X, and 20X LWD PH objectives that are ideal for tissue culture microscopy
• A fixed stage for flexibility in imaging different cell culture vessels
• Fits and operates inside cell culture hoods
• All-in-one design: digital camera, precision optics, LCD display, and USB storage

Imaging anywhere
The EVOS XL Core system is an integrated transmitted-light inverted imaging system that combines high-quality optics, a 12.1-inch high-resolution LCD display, and a digital color camera. Images are seamlessly acquired through the user interface using a mouse and the integrated software that includes a variety of features, such as color temperature control. All images can be saved onto the USB device in JPEG, BMP, or TIFF formats. Applications suited for the EVOS XL Core system include basic imaging of live and fixed cells, tissue culture needs (confluence, density, and growth), stem cell isolation, and analysis of stained tissue slices.

The EVOS imaging systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy this system is to operate and amazed how good your images look.

Versatile
While the microscope comes equipped with three objectives (4X, 10X, and 20X LWD PH), the four-position objective turret can be configured to meet additional needs using our full range of LWD phase contrast and coverslip-corrected objectives. LWD PH objectives are available from 1.25X to 40X. The compact footprint makes it easy to use the EVOS XL Core system wherever needed; the entire system can be easily moved into a cell culture hood or glove box. The universal stage provides maximum flexibility for slides, culture dishes, and flasks. An optional mechanical stage affords more precise handling and positioning of multi-well plate vessels.

Easy to use and reliable
The EVOS XL Core Imaging System is plug-and-play. In addition to an exceptionally easy set-up and installation, it requires no warm-up or cool-down periods. The LED light source offers exceptional stability and durability--so you can turn the unit on and off whenever you need to image a sample. The LED bulbs are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long lifetime and low energy consumption translate into lower operating costs compared to instruments with conventional light sources. Finally, the advanced ergonomic design of the EVOS systems removes the strain of conventional microscopes in imaging, enables shared viewing, and makes moving the units easy.

Learn more about the EVOS XL Core Imaging System and most recent software ›
See the optional mechanical stage ›
Explore the entire EVOS line of imaging systems and accessories ›

Unit includes:
• EVOS XL Core Imaging System with Fixed Stage
• EVOS Obj, Inf Plan Achro 4X LWD PH, 0.13NA/16.9WD (AMEP4632)
• EVOS Obj, Inf Plan Achro 10X LWD PH, 0.25NA/6.9WD (AMEP4633)
• EVOS Obj, Inf Plan Achro 20X LWD PH, 0.40NA/6.8WD (AMEP4634)
• Glass stage plate
• Wireless mouse
• Power adaptor, cord, and universal power plug adapters
• USB flash drive (includes User Guide and Quick Start Guide)
• Dust cover
• 3-mm hex driver L-shaped key

System highlights

Optics: infinity-corrected optical system, RMS-threaded objectives with 45-mm parfocal distance
Objectives: 4X, 10X, and 20X LWD PH achromat objectives. A wide selection of other high-quality LWD and coverslip-corrected objectives is available.
Illumination: adjustable intensity LED (>50,000-hour life)
Contrast methods: transmitted light (brightfield and phase contrast)
Objective turret: 4-position, manual control
Condenser: 3-position turret for brightfield and phase contrast
Condenser working distance: 60 mm
Stage: fixed stage
Focus mechanism: coaxial focus knobs with tension control. Course focus: 38 mm/rev. Fine focus: 0.2 mm/rev, precision 0.002 mm.
LCD display: 12.1-inch high-resolution (1024 x 768 pixels) color monitor with adjustable tilt
Camera: color, 2048 x 1536, 3.1 megapixels
Image acquisition: embedded operating system with software for image capture and saving via mouse or front-mounted manual buttons
Captured images Color camera: 24-bit full color TIFF or PNG; jpeg, bmp (2048 x 1536 pixels)
Output ports: 2 USB 2.0 ports
Power supply: AC adaptor; Input: 100-240 V, 47-63 Hz; Output: 12 V DC/2.0 A, 24W max
Dimensions: Height: 533 mm (21.0 in); Depth: 406 mm (6.0 in); Width: 318 mm (12.5 in)
Weight: 9.1 kg (20.1 lbs)

EVOS™ FL Auto Calibration Slide (Invitrogen™)

The calibration slide for the EVOS FL Auto Imaging System is used to calibrate three parameters of the system: field of view, parfocality, and parcentration. Calibrating the parfocality ensures that the sample stays in focus when the objective is changed, and calibrating the parcentration ensures that an object in the center of the field of view will stay in the center of the field no matter which objective is being used.

The calibration slide for the EVOS FL Auto Imaging System offers you these advantages:
• Easy, accurate, and precise calibration of your microscope
• Five X-Y squares divided by grids of: 1.00, 0.200, 0.100, 0.050, and 0.025 mm
• Seven different calibration circles: 4.000, 2.000, 1.000, 0.400, 0.200, 0.100, and 0.050 mm
• Precision-ground, optical-quality glass
• Protective storage case

The calibration procedure involves two distinct steps. First, the calibration crosshair on the screen is matched to the crosshairs on the calibration slide. Then, the diameters of the reference circles on the calibration slide are measured. Each step is performed using both the monochrome and the color camera. The total time required to calibrate an objective is about 5 minutes. More information can be found in the EVOS FL Auto manual. Note that the factory-installed objectives supplied with the EVOS FL Auto Imaging System have been pre-calibrated. There is no need to calibrate them again unless they are removed and need to be re-installed.

Learn more about the EVOS FL Auto Imaging System >.
Explore the entire EVOS line of imaging systems and accessories >.

EVOS™ Light Cube, Qdot™ WB, 525-800

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot WB (Wide Bandpass) light cube is compatible with all Qdot fluorophores and any other fluorophores that have excitation and emission maxima near 445 and 525-800 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Power Cord, Type H (Israel)

This power cord is for use with EVOS XL, FL , FL Color, and FL Auto systems in countries with Type H power plugs. The Type H plug is used in Israel and Gaza.

See the complete list of power cord types and countries for more information.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 100X Oil Objective, achromat, coverslip-corrected

This achromat objective is ideal for general applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 100X
• Numerical Aperture: 1.25
• Working Distance: 0.15 mm
• Immersion: Oil

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Light Cube, Qdot™ 625

All EVOS fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The Qdot 625 light cube is ideal for use with Qdot 625 and other fluorophores with excitation and emission maxima near 445 and 625 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.