Product FAQ

In the catalog, it is said that Hanks' Balanced Salt solutions are intended for use in ambient air, not in CO2 equilibrated incubator.What is the reason for that? Also, what is the functional difference between Hanks' Balanced Salt Solutions and Earle's Balanced Salt Solutions?

Answer

The basic difference between Earle's salts and Hanks' salts is the level of sodium bicarbonate. It is much higher in Earle's (2.2g/L) than in Hanks' (0.35g/L). Sodium bicarbonate balances with high levels of CO2 (5%) to hold the pH of a solution. If Earle's salts are left in ambient levels of CO2 a solution will become alkaline whereas a Hanks' buffered solution in a CO2 incubator will become acidic. If you wish to store tissue in the CO2 incubator, you need to use Earle's. However if you are simply rinsing tissue that will be stored later in cell culture medium, Hanks' will be fine.

Answer Id: E3170

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