For Research Use Only. Not for use in diagnostic procedures., View all Does SimplyBlue™ SafeStain contain Coomassie™ G250 or Coomassie™ R250? Can I dry my SYPRO™ Ruby, SYPRO™ Orange, or SYPRO™ Red stained gels?
We offer a wide variety of staining and drying accessories to help you process your protein gel electrophoresis gels., The StainEase® Staining Tray is a convenient way to evenly stain minigels and prevent gels from breaking. Easily fits up to 4 mini- or midi-gels.
Once proteins have been separated by electrophoresis, they can be visualized using different methods of in-gel detection, each with advantages and disadvantages. Over the past several decades, demand for improved sensitivity for small sample sizes and compatibility with downstream applications and...
Invitrogen SYBR Safe DNA Gel Stain is a highly sensitive dye for visualizing DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be viewed with blue-light or by UV excitation.
Invitrogen Novex high-quality reagents and devices provide specific and sensitive purification, separation and detection of proteins while delivering unambiguous, clean results. These products are designed to achieve experimental accuracy and consistency as well as simplify and streamline the...
Molecular Probes® fluorescent nucleic acid gel stains—SYBR® Gold, SYBR® Green I, SYBR® Green II, and SYBR® Safe dyes—are highly sensitive reagents for staining RNA in electrophoresis gels. These gel stains provide greater sensitivity, with lower background fluorescence, than the conventional...
Fluorescent stains are rapid, and highly sensitive for detecting total protein in protein electrophoresis gels and membranes. Fluorescent stains are designed for use in 1D and 2D PAGE and offer sensitivities similar to that obtained with silver staining techniques.
In the nucleic acid electrophoresis workflow, several steps are performed sequentially, each of which could impact the outcome of nucleic acid separation. This article addresses five considerations associated with samples, reagents, and running parameters.
In gel electrophoresis, fluorescent nucleic acid gel stains are dyes used for DNA detection. When bound to DNA molecules and illuminated with UV or blue light, these dye molecules fluoresce brightly. Once the gel is stained, transilluminators are used to excite the dye molecules, which allow DNA to...
In biolaboratories, agarose gel electrophoresis is the modus operandi for size-based separation of DNA and RNA fragments. The agarose gel matrix is porous and acts as a sieve through which the nucleic acid molecules migrate.
Gel electrophoresis is a common laboratory technique to isolate nucleic acids and proteins. Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. Gel electrophoresis is vital in all molecular biology workflows.
DNA electrophoresis is a standard laboratory technique used to identify, quantify, and purify DNA fragments. DNA electrophoresis involves loading DNA samples into the wells of an agarose or acrylamide gel and subjecting it to an electric field.
Two-dimensional gel electrophoresis (2DE) is an established technique for high-resolution profiling of complex protein mixtures. 2DE can resolve thousands of protein “spots” on a single gel and is considered a key method in proteomics research.
We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
Most life science researchers will run a gel at some point in their careers. Running a gel, formally called gel electrophoresis, is an effective method to separate biomolecules, such as nucleic acids and proteins , based on size.