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VEGF Receptor 1 (Flt1) Monoclonal Antibody (7A6), eBioscience™ (Invitrogen™)

VEGF Receptor 1 (Flt1) Monoclonal Antibody for Western Blot

VEGF Receptor 1 (Flt1) Monoclonal Antibody (Flt-19), eBioscience™ (Invitrogen™)

VEGF Receptor 1 (Flt1) Monoclonal Antibody for IHC, IP, ELISA

FLT-3L Human ELISA Kit (Invitrogen™)

FLT-3L Human ELISA Kit for ELISA

Lab Vision™ Flt-1/VEGFR1 Ab-1, Rabbit Polyclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in western blotting experiments with Thermo Scientific™ Flt-1/VEGFR1 Ab-1, Rabbit Polyclonal Antibody.

pcDNA™5/FRT/TO Vector Kit (Invitrogen™)

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.

The pcDNA™5⁄FRT⁄TO vector contains the following elements:

• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell lines

FLT-3L Mouse ELISA Kit (Invitrogen™)

FLT-3L Mouse ELISA Kit for ELISA

pEF5/FRT/V5-DEST™ Gateway™ Vector (Invitrogen™)

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, and reaction buffer.

Flt-3 Ligand Human ProQuantum Immunoassay Kit (Invitrogen™)

Flt-3 Ligand Human ProQuantum Immunoassay Kit

GeneArt™ Service Mutagenesis Bundle Cloning into pcDNA™5/FRT Vector

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

pEF5/FRT/V5 Directional TOPO™ Cloning Kit (Invitrogen™)

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).

Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.

FLT-3L Monkey ELISA Kit (Invitrogen™)

FLT-3L Monkey ELISA Kit for ELISA

pcDNA™5/FRT/TO TOPO™ TA Expression Kit (Invitrogen™)

pcDNA™5/FRT/TO, pcDNA5/FRT/TO-TOPO® and pcDNA™5/FRT/TO-E (Figure 1) are designed for targeted Flp-In™ integration followed by tetracycline-regulated expression. Each vector contains a FRT site for efficient targeted integration and a hybrid CMV/TetO2 promoter for regulated expression. In addition, pcDNA5/FRT/TO-TOPO® and pcDNA™5/FRT/TO-E are adapted for TOPO®-and Echo™ Cloning, respectively. TOPO® Cloning enables rapid ligation of PCR products into pcDNA5/FRT/TO-TOPO® in just five minutes on your bench top. Echo™ Cloning allows you to quickly shuttle your gene of interest into multiple expression vectors.
The Flp-In™ T-REx™ 293 Cell Line is available to save time in expression experiments. This cell line contains pFRT/lacZeo and pcDNA™6/TR stably integrated and is tested for its ability to regulate expression (Figure 2).

pSecTag/FRT/V5-His© TOPO™ TA Expression Kit (Invitrogen™)

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).

Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.

pcDNA™5/FRT/V5-His-TOPO™ TA Expression Kit (Invitrogen™)

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).

Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.

FLT1 Monoclonal Antibody (OTI11G5), TrueMAB™ (OriGene)

FLT1 Monoclonal Antibody for Western blot